Exploring bioactivities and peptide content of body mucus from the lusitanian toadfish Halobatrachus didactylus
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.12/9620 |
Resumo: | Identifying bioactive molecules from marine organisms is still vastly understudied. Fish remain an untapped source of bioactive molecules, even when considering species whose toxicity to other fish species has been noticed before. We assessed potential applications of crude body mucus of the Lusitanian toadfish (Halobratachus didactylus) and characterized its peptide fraction composition. Mucus samples from three individuals (two wild and one captive) revealed potential antioxidant, antihypertensive, and antimicrobial activities. For antioxidant activity, the best results of 2371 ± 97 µmol Trolox Equivalent/g protein for ORAC and 154 ± 6 µmol Trolox Equivalent/g protein for ABTS were obtained. For antihypertensive activity, the relevant inhibitory activity of ACE resulted in IC50 of 60 ± 7 µg protein/mL. Antimicrobial activity was also identified against the pathogenic bacteria Escherichia coli and Listeria monocytogenes. The peptide profile of the crude body mucus was obtained through size exclusion chromatography, with a conspicuous peak at ca. 800 Da. LC-MS/MS allowed the detection of the most probable peptide sequences of this dominant peptide. This is the first study where the bioactive potential of mucus from the Lusitanian toadfish is demonstrated. Peptides with such properties can be applied in the food and pharmaceutical industries. |
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Exploring bioactivities and peptide content of body mucus from the lusitanian toadfish Halobatrachus didactylusIchthyocrinotoxinsAntimicrobialAntioxidantAntihypertensivePeptidesIdentifying bioactive molecules from marine organisms is still vastly understudied. Fish remain an untapped source of bioactive molecules, even when considering species whose toxicity to other fish species has been noticed before. We assessed potential applications of crude body mucus of the Lusitanian toadfish (Halobratachus didactylus) and characterized its peptide fraction composition. Mucus samples from three individuals (two wild and one captive) revealed potential antioxidant, antihypertensive, and antimicrobial activities. For antioxidant activity, the best results of 2371 ± 97 µmol Trolox Equivalent/g protein for ORAC and 154 ± 6 µmol Trolox Equivalent/g protein for ABTS were obtained. For antihypertensive activity, the relevant inhibitory activity of ACE resulted in IC50 of 60 ± 7 µg protein/mL. Antimicrobial activity was also identified against the pathogenic bacteria Escherichia coli and Listeria monocytogenes. The peptide profile of the crude body mucus was obtained through size exclusion chromatography, with a conspicuous peak at ca. 800 Da. LC-MS/MS allowed the detection of the most probable peptide sequences of this dominant peptide. This is the first study where the bioactive potential of mucus from the Lusitanian toadfish is demonstrated. Peptides with such properties can be applied in the food and pharmaceutical industries.Fundação Para a Ciência e Tecnologia - FCTMDPIRepositório do ISPACunha, Marta FernandezCoscueta, EzequielEmilia Brassesco, M.Marques, RitaNeto, JoséAlmada, Frederico José Oliveira deGoncalves, DavidPintado, Maria Manuela2024-02-27T19:18:43Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.12/9620engFernandez Cunha, M., Coscueta, E. R., Brassesco, M. E., Marques, R., Neto, J., Almada, F., Gonçalves, D., & Pintado, M. (2023). Exploring Bioactivities and Peptide Content of Body Mucus from the Lusitanian Toadfish Halobatrachus didactylus. Molecules, 28(18), 6458. https://doi.org/10.3390/molecules281864581420304910.3390/molecules28186458info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-03T02:15:40Zoai:repositorio.ispa.pt:10400.12/9620Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:12:20.909864Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Exploring bioactivities and peptide content of body mucus from the lusitanian toadfish Halobatrachus didactylus |
title |
Exploring bioactivities and peptide content of body mucus from the lusitanian toadfish Halobatrachus didactylus |
spellingShingle |
Exploring bioactivities and peptide content of body mucus from the lusitanian toadfish Halobatrachus didactylus Cunha, Marta Fernandez Ichthyocrinotoxins Antimicrobial Antioxidant Antihypertensive Peptides |
title_short |
Exploring bioactivities and peptide content of body mucus from the lusitanian toadfish Halobatrachus didactylus |
title_full |
Exploring bioactivities and peptide content of body mucus from the lusitanian toadfish Halobatrachus didactylus |
title_fullStr |
Exploring bioactivities and peptide content of body mucus from the lusitanian toadfish Halobatrachus didactylus |
title_full_unstemmed |
Exploring bioactivities and peptide content of body mucus from the lusitanian toadfish Halobatrachus didactylus |
title_sort |
Exploring bioactivities and peptide content of body mucus from the lusitanian toadfish Halobatrachus didactylus |
author |
Cunha, Marta Fernandez |
author_facet |
Cunha, Marta Fernandez Coscueta, Ezequiel Emilia Brassesco, M. Marques, Rita Neto, José Almada, Frederico José Oliveira de Goncalves, David Pintado, Maria Manuela |
author_role |
author |
author2 |
Coscueta, Ezequiel Emilia Brassesco, M. Marques, Rita Neto, José Almada, Frederico José Oliveira de Goncalves, David Pintado, Maria Manuela |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório do ISPA |
dc.contributor.author.fl_str_mv |
Cunha, Marta Fernandez Coscueta, Ezequiel Emilia Brassesco, M. Marques, Rita Neto, José Almada, Frederico José Oliveira de Goncalves, David Pintado, Maria Manuela |
dc.subject.por.fl_str_mv |
Ichthyocrinotoxins Antimicrobial Antioxidant Antihypertensive Peptides |
topic |
Ichthyocrinotoxins Antimicrobial Antioxidant Antihypertensive Peptides |
description |
Identifying bioactive molecules from marine organisms is still vastly understudied. Fish remain an untapped source of bioactive molecules, even when considering species whose toxicity to other fish species has been noticed before. We assessed potential applications of crude body mucus of the Lusitanian toadfish (Halobratachus didactylus) and characterized its peptide fraction composition. Mucus samples from three individuals (two wild and one captive) revealed potential antioxidant, antihypertensive, and antimicrobial activities. For antioxidant activity, the best results of 2371 ± 97 µmol Trolox Equivalent/g protein for ORAC and 154 ± 6 µmol Trolox Equivalent/g protein for ABTS were obtained. For antihypertensive activity, the relevant inhibitory activity of ACE resulted in IC50 of 60 ± 7 µg protein/mL. Antimicrobial activity was also identified against the pathogenic bacteria Escherichia coli and Listeria monocytogenes. The peptide profile of the crude body mucus was obtained through size exclusion chromatography, with a conspicuous peak at ca. 800 Da. LC-MS/MS allowed the detection of the most probable peptide sequences of this dominant peptide. This is the first study where the bioactive potential of mucus from the Lusitanian toadfish is demonstrated. Peptides with such properties can be applied in the food and pharmaceutical industries. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023 2023-01-01T00:00:00Z 2024-02-27T19:18:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.12/9620 |
url |
http://hdl.handle.net/10400.12/9620 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fernandez Cunha, M., Coscueta, E. R., Brassesco, M. E., Marques, R., Neto, J., Almada, F., Gonçalves, D., & Pintado, M. (2023). Exploring Bioactivities and Peptide Content of Body Mucus from the Lusitanian Toadfish Halobatrachus didactylus. Molecules, 28(18), 6458. https://doi.org/10.3390/molecules28186458 14203049 10.3390/molecules28186458 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137772541313024 |