The impact of biomass withdrawal strategy on the biomass selection and polyhydroxyalkanoates accumulation of mixed microbial cultures

Detalhes bibliográficos
Autor(a) principal: Cruz, Rafaela A. P.
Data de Publicação: 2022
Outros Autores: Oehmen, Adrian, Reis, Maria A. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/134345
Resumo: UIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020 SFRH/BD/110673/2015
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spelling The impact of biomass withdrawal strategy on the biomass selection and polyhydroxyalkanoates accumulation of mixed microbial culturesBiomass withdrawalBioplasticsMixed microbial cultures (MMC)Polyhydroxyalkanoates (PHA)Process optimizationSequencing batch reactors (SBR)BiotechnologyBioengineeringMolecular BiologyUIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020 SFRH/BD/110673/2015The production of polyhydroxyalkanoates (PHA) by mixed microbial cultures (MMC) has been studied as an alternative to pure cultures in order to reduce the price of PHA through use of open systems and low-cost substrates, such as agro-industrial sub-products. However, the widespread applicability of this process depends on the optimization of operational factors impacting PHA productivity. This study addresses the impact of biomass withdrawal strategy on the performance of MMC selection reactors and consequently on biomass productivity and global PHA productivity. Two selection reactors were operated in parallel under similar conditions, except for the timing of biomass withdrawal, at the end of the famine phase (Reactor 1, R1) versus at the end of the feast phase (Reactor 2, R2) at an organic loading rate of 100 Cmmol.L−1.d−1 and solids retention time of 4 days. The biomass selected in both conditions had similar PHA storing capacity as shown by the similar yields of PHA per substrate obtained in the accumulation assays; however, R1 reached a higher biomass productivity (about 4-fold higher than R2). This study demonstrated that removing the excess biomass at the end of the famine phase resulted in a much higher global PHA productivity and that the key parameter affecting the global PHA productivity of the 2-stage system was the volumetric biomass productivity. Results obtained provide important insight into how MMC systems can be best operated to maximize PHA productivity.DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitRUNCruz, Rafaela A. P.Oehmen, AdrianReis, Maria A. M.2022-03-11T23:22:34Z2022-01-252022-01-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://hdl.handle.net/10362/134345eng1871-6784PURE: 36690863https://doi.org/10.1016/j.nbt.2021.08.004info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:12:48Zoai:run.unl.pt:10362/134345Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:48:04.288625Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The impact of biomass withdrawal strategy on the biomass selection and polyhydroxyalkanoates accumulation of mixed microbial cultures
title The impact of biomass withdrawal strategy on the biomass selection and polyhydroxyalkanoates accumulation of mixed microbial cultures
spellingShingle The impact of biomass withdrawal strategy on the biomass selection and polyhydroxyalkanoates accumulation of mixed microbial cultures
Cruz, Rafaela A. P.
Biomass withdrawal
Bioplastics
Mixed microbial cultures (MMC)
Polyhydroxyalkanoates (PHA)
Process optimization
Sequencing batch reactors (SBR)
Biotechnology
Bioengineering
Molecular Biology
title_short The impact of biomass withdrawal strategy on the biomass selection and polyhydroxyalkanoates accumulation of mixed microbial cultures
title_full The impact of biomass withdrawal strategy on the biomass selection and polyhydroxyalkanoates accumulation of mixed microbial cultures
title_fullStr The impact of biomass withdrawal strategy on the biomass selection and polyhydroxyalkanoates accumulation of mixed microbial cultures
title_full_unstemmed The impact of biomass withdrawal strategy on the biomass selection and polyhydroxyalkanoates accumulation of mixed microbial cultures
title_sort The impact of biomass withdrawal strategy on the biomass selection and polyhydroxyalkanoates accumulation of mixed microbial cultures
author Cruz, Rafaela A. P.
author_facet Cruz, Rafaela A. P.
Oehmen, Adrian
Reis, Maria A. M.
author_role author
author2 Oehmen, Adrian
Reis, Maria A. M.
author2_role author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Cruz, Rafaela A. P.
Oehmen, Adrian
Reis, Maria A. M.
dc.subject.por.fl_str_mv Biomass withdrawal
Bioplastics
Mixed microbial cultures (MMC)
Polyhydroxyalkanoates (PHA)
Process optimization
Sequencing batch reactors (SBR)
Biotechnology
Bioengineering
Molecular Biology
topic Biomass withdrawal
Bioplastics
Mixed microbial cultures (MMC)
Polyhydroxyalkanoates (PHA)
Process optimization
Sequencing batch reactors (SBR)
Biotechnology
Bioengineering
Molecular Biology
description UIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020 SFRH/BD/110673/2015
publishDate 2022
dc.date.none.fl_str_mv 2022-03-11T23:22:34Z
2022-01-25
2022-01-25T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/134345
url http://hdl.handle.net/10362/134345
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1871-6784
PURE: 36690863
https://doi.org/10.1016/j.nbt.2021.08.004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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