Water and carbon dioxide: green solvents for the extraction of collagen/gelatin from marine sponges

Detalhes bibliográficos
Autor(a) principal: Barros, Alexandre A.
Data de Publicação: 2015
Outros Autores: Aroso, Ivo Manuel Ascensão, Silva, Tiago H., Mano, J. F., Duarte, Ana Rita C., Reis, R. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/33050
Resumo: "Publication Date (Web): December 23, 2014"
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spelling Water and carbon dioxide: green solvents for the extraction of collagen/gelatin from marine spongesGreen solventsExtractionCollagenGelatinMarine spongesCarbon dioxideWaterNatural productsScience & Technology"Publication Date (Web): December 23, 2014"Marine sponges are extremely rich in natural products and are considered a promising biological resource. The major objective of this work is to couple a green extraction process with a natural origin raw material to obtain sponge origin collagen/gelatin for biomedical applications. Marine sponge collagen has unique physicochemical properties, but its application is hindered by the lack of availability due to inefficient extraction methodologies. Traditional extraction methods are time consuming as they involve several operating steps and large amounts of solvents. In this work, we propose a new extraction methodology under mild operating conditions in which water is acidified with carbon dioxide (CO2) to promote the extraction of collagen/gelatin from different marine sponge species. An extraction yield of approximately 50% of collagen/gelatin was achieved. The results of Fourier transformed infrared spectroscopy (FTIR), circular dichroism (CD), and differential scanning calorimetry (DSC) spectra suggest a mixture of collagen/gelatin with high purity, and the analysis of the amino acid composition has shown similarities with collagen from other marine sources. Additionally, in vitro cytotoxicity studies did not demonstrate any toxicity effects for three of the extracts.The authors are grateful for financial support of FCT through Grant EXP/QEQ:EPS/0745/2012, SWIMS (Subcritical Water Isolation of compounds from Marine Sponges). The funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement numbers REGPOT-CT2012-316331-POLARIS and KBBE-2010-266033 (project SPECIAL), as well as from ERDF under the project "Novel smart and biomimetic materials for innovative regenerative medicine approaches" RLI-ABMR-NORTE-01-0124-FEDER-000016), cofinanced by North Portugal Regional Operational Programme (ON.2,O Novo Norte), under the National Strategic Reference Framework (NSRF) are also gratefully ackowledged. The authors are also truly thankfull to Prof. Micha flan (Tel Aviv University, Israel), Dr. Ronald Osinga (Porifarma, The Netherlands), Dr. Antonio Sara and Dr. Martina Milanese (Studio Associato GAIA, Italy), and Dr. Joana Xavier (University of Azores) for the kind offer of marine sponges samples.ACS PublicationsUniversidade do MinhoBarros, Alexandre A.Aroso, Ivo Manuel AscensãoSilva, Tiago H.Mano, J. F.Duarte, Ana Rita C.Reis, R. L.20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/33050engBarros, A. A., Aroso, I. M., Silva, T. H., Mano, J. F., Duarte, A. R. C., & Reis, R. L. (2015). Water and Carbon Dioxide: Green Solvents for the Extraction of Collagen/Gelatin from Marine Sponges. Acs Sustainable Chemistry & Engineering, 3(2), 254-260. doi: 10.1021/sc500621z2168-048510.1021/sc500621zhttp://pubs.acs.org/doi/abs/10.1021/sc500621zinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:54:36Zoai:repositorium.sdum.uminho.pt:1822/33050Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:54:36Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Water and carbon dioxide: green solvents for the extraction of collagen/gelatin from marine sponges
title Water and carbon dioxide: green solvents for the extraction of collagen/gelatin from marine sponges
spellingShingle Water and carbon dioxide: green solvents for the extraction of collagen/gelatin from marine sponges
Barros, Alexandre A.
Green solvents
Extraction
Collagen
Gelatin
Marine sponges
Carbon dioxide
Water
Natural products
Science & Technology
title_short Water and carbon dioxide: green solvents for the extraction of collagen/gelatin from marine sponges
title_full Water and carbon dioxide: green solvents for the extraction of collagen/gelatin from marine sponges
title_fullStr Water and carbon dioxide: green solvents for the extraction of collagen/gelatin from marine sponges
title_full_unstemmed Water and carbon dioxide: green solvents for the extraction of collagen/gelatin from marine sponges
title_sort Water and carbon dioxide: green solvents for the extraction of collagen/gelatin from marine sponges
author Barros, Alexandre A.
author_facet Barros, Alexandre A.
Aroso, Ivo Manuel Ascensão
Silva, Tiago H.
Mano, J. F.
Duarte, Ana Rita C.
Reis, R. L.
author_role author
author2 Aroso, Ivo Manuel Ascensão
Silva, Tiago H.
Mano, J. F.
Duarte, Ana Rita C.
Reis, R. L.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Barros, Alexandre A.
Aroso, Ivo Manuel Ascensão
Silva, Tiago H.
Mano, J. F.
Duarte, Ana Rita C.
Reis, R. L.
dc.subject.por.fl_str_mv Green solvents
Extraction
Collagen
Gelatin
Marine sponges
Carbon dioxide
Water
Natural products
Science & Technology
topic Green solvents
Extraction
Collagen
Gelatin
Marine sponges
Carbon dioxide
Water
Natural products
Science & Technology
description "Publication Date (Web): December 23, 2014"
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/33050
url http://hdl.handle.net/1822/33050
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Barros, A. A., Aroso, I. M., Silva, T. H., Mano, J. F., Duarte, A. R. C., & Reis, R. L. (2015). Water and Carbon Dioxide: Green Solvents for the Extraction of Collagen/Gelatin from Marine Sponges. Acs Sustainable Chemistry & Engineering, 3(2), 254-260. doi: 10.1021/sc500621z
2168-0485
10.1021/sc500621z
http://pubs.acs.org/doi/abs/10.1021/sc500621z
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ACS Publications
publisher.none.fl_str_mv ACS Publications
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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