Endothelial progenitor cells in diabetic patients with myocardial infarction – Can statins improve their function?
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/27834 https://doi.org/10.1016/j.ejphar.2014.07.010 |
Resumo: | The effect of statins on endothelial progenitor cells (EPCs) function derived from diabetic patients (DMpts) with acute myocardial infarction (AMI) is unknown. In this study we assess the response of early and late EPCs from diabetic versus non-diabetic patients (NDMpts) with AMI to statins. EPCs were obtained from 10 diabetic and 10 age-matched non-diabetic male patients with AMI. For each patient, cultures of early and late EPCs were performed under 4 different conditions: normal glucose concentration (control); high glucose concentration; normal glucose concentration with atorvastatin supplementation and normal glucose concentration with pravastatin supplementation. To compare the effect of these treatments on EPC function in DMpts versus NDMpts, we performed in vitro: EPC colony-forming units (CFU) assay; cell cycle analysis; viability assessment and expression of the surface markers CXCR4, CD133, CD34 and KDR. Under control conditions, CFU numbers were reduced in DMpts-derived EPCs when compared to those of NDMpts (1.4±0.8 vs 2.6±1.2 CFU/well, P=0.021). When early EPCs from DMpts were cultured in the presence of statins, CFU capacity was restored, surmounting that of NDMpts under control conditions. Statins significantly improved viability of early EPCs and delayed the onset of late EPCs senescence, even in cells from DMpts. In addition, statins induced approximately a 2-fold increase in the proportion of late EPCs in S-phase of the cell cycle (P<0.05). Statins have a beneficial effect on both early and late EPCs from DMpts with AMI. Despite the functional impairment of EPCs from DMpts, they exhibit similar responsiveness to statins as equivalent cells from NDMpts. |
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Endothelial progenitor cells in diabetic patients with myocardial infarction – Can statins improve their function?Endothelial progenitor cellsDiabetes mellitusMyocardial infarctionStatinsFunctionThe effect of statins on endothelial progenitor cells (EPCs) function derived from diabetic patients (DMpts) with acute myocardial infarction (AMI) is unknown. In this study we assess the response of early and late EPCs from diabetic versus non-diabetic patients (NDMpts) with AMI to statins. EPCs were obtained from 10 diabetic and 10 age-matched non-diabetic male patients with AMI. For each patient, cultures of early and late EPCs were performed under 4 different conditions: normal glucose concentration (control); high glucose concentration; normal glucose concentration with atorvastatin supplementation and normal glucose concentration with pravastatin supplementation. To compare the effect of these treatments on EPC function in DMpts versus NDMpts, we performed in vitro: EPC colony-forming units (CFU) assay; cell cycle analysis; viability assessment and expression of the surface markers CXCR4, CD133, CD34 and KDR. Under control conditions, CFU numbers were reduced in DMpts-derived EPCs when compared to those of NDMpts (1.4±0.8 vs 2.6±1.2 CFU/well, P=0.021). When early EPCs from DMpts were cultured in the presence of statins, CFU capacity was restored, surmounting that of NDMpts under control conditions. Statins significantly improved viability of early EPCs and delayed the onset of late EPCs senescence, even in cells from DMpts. In addition, statins induced approximately a 2-fold increase in the proportion of late EPCs in S-phase of the cell cycle (P<0.05). Statins have a beneficial effect on both early and late EPCs from DMpts with AMI. Despite the functional impairment of EPCs from DMpts, they exhibit similar responsiveness to statins as equivalent cells from NDMpts.Elsevier2014-10-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/27834http://hdl.handle.net/10316/27834https://doi.org/10.1016/j.ejphar.2014.07.010engANTÓNIO, Natália [et. al] - Endothelial progenitor cells in diabetic patients with myocardial infarction – Can statins improve their function?. "European Journal of Pharmacology". ISSN 0014-2999. Vol. 741 (2014) p. 25–360014-2999http://www.sciencedirect.com/science/article/pii/S0014299914005470António, NatáliaSoares, AnaFernandes, RosaSoares, FranciscoLopes, AnaCarvalheiro, TiagoPaiva, ArturProvidência, Luís A.Gonçalves, LinoRibeiro, Carlos Fontesinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-05-29T09:42:29Zoai:estudogeral.uc.pt:10316/27834Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:53:39.942035Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Endothelial progenitor cells in diabetic patients with myocardial infarction – Can statins improve their function? |
title |
Endothelial progenitor cells in diabetic patients with myocardial infarction – Can statins improve their function? |
spellingShingle |
Endothelial progenitor cells in diabetic patients with myocardial infarction – Can statins improve their function? António, Natália Endothelial progenitor cells Diabetes mellitus Myocardial infarction Statins Function |
title_short |
Endothelial progenitor cells in diabetic patients with myocardial infarction – Can statins improve their function? |
title_full |
Endothelial progenitor cells in diabetic patients with myocardial infarction – Can statins improve their function? |
title_fullStr |
Endothelial progenitor cells in diabetic patients with myocardial infarction – Can statins improve their function? |
title_full_unstemmed |
Endothelial progenitor cells in diabetic patients with myocardial infarction – Can statins improve their function? |
title_sort |
Endothelial progenitor cells in diabetic patients with myocardial infarction – Can statins improve their function? |
author |
António, Natália |
author_facet |
António, Natália Soares, Ana Fernandes, Rosa Soares, Francisco Lopes, Ana Carvalheiro, Tiago Paiva, Artur Providência, Luís A. Gonçalves, Lino Ribeiro, Carlos Fontes |
author_role |
author |
author2 |
Soares, Ana Fernandes, Rosa Soares, Francisco Lopes, Ana Carvalheiro, Tiago Paiva, Artur Providência, Luís A. Gonçalves, Lino Ribeiro, Carlos Fontes |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
António, Natália Soares, Ana Fernandes, Rosa Soares, Francisco Lopes, Ana Carvalheiro, Tiago Paiva, Artur Providência, Luís A. Gonçalves, Lino Ribeiro, Carlos Fontes |
dc.subject.por.fl_str_mv |
Endothelial progenitor cells Diabetes mellitus Myocardial infarction Statins Function |
topic |
Endothelial progenitor cells Diabetes mellitus Myocardial infarction Statins Function |
description |
The effect of statins on endothelial progenitor cells (EPCs) function derived from diabetic patients (DMpts) with acute myocardial infarction (AMI) is unknown. In this study we assess the response of early and late EPCs from diabetic versus non-diabetic patients (NDMpts) with AMI to statins. EPCs were obtained from 10 diabetic and 10 age-matched non-diabetic male patients with AMI. For each patient, cultures of early and late EPCs were performed under 4 different conditions: normal glucose concentration (control); high glucose concentration; normal glucose concentration with atorvastatin supplementation and normal glucose concentration with pravastatin supplementation. To compare the effect of these treatments on EPC function in DMpts versus NDMpts, we performed in vitro: EPC colony-forming units (CFU) assay; cell cycle analysis; viability assessment and expression of the surface markers CXCR4, CD133, CD34 and KDR. Under control conditions, CFU numbers were reduced in DMpts-derived EPCs when compared to those of NDMpts (1.4±0.8 vs 2.6±1.2 CFU/well, P=0.021). When early EPCs from DMpts were cultured in the presence of statins, CFU capacity was restored, surmounting that of NDMpts under control conditions. Statins significantly improved viability of early EPCs and delayed the onset of late EPCs senescence, even in cells from DMpts. In addition, statins induced approximately a 2-fold increase in the proportion of late EPCs in S-phase of the cell cycle (P<0.05). Statins have a beneficial effect on both early and late EPCs from DMpts with AMI. Despite the functional impairment of EPCs from DMpts, they exhibit similar responsiveness to statins as equivalent cells from NDMpts. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/27834 http://hdl.handle.net/10316/27834 https://doi.org/10.1016/j.ejphar.2014.07.010 |
url |
http://hdl.handle.net/10316/27834 https://doi.org/10.1016/j.ejphar.2014.07.010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
ANTÓNIO, Natália [et. al] - Endothelial progenitor cells in diabetic patients with myocardial infarction – Can statins improve their function?. "European Journal of Pharmacology". ISSN 0014-2999. Vol. 741 (2014) p. 25–36 0014-2999 http://www.sciencedirect.com/science/article/pii/S0014299914005470 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133822978097152 |