Carbon film electrodes for oxidase-based enzyme sensors in food analysis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2005 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5095 https://doi.org/10.1016/j.talanta.2005.06.017 |
Resumo: | Carbon film resistor electrodes have been evaluated as transducers for the development of multiple oxidase-based enzyme electrode biosensors. The resistor electrodes were first modified with Prussian Blue (PB) and then covered by a layer of covalently immobilized enzyme. Electrochemical impedance spectroscopy was used to characterize the interfacial behaviour of the Prussian Blue modified and enzyme electrodes; the spectra demonstrated that the access of the substrates is essentially unaltered by application of the enzyme layer. These enzyme electrodes were used to detect the substrate of the oxidase (glucose, ethanol, lactate, glutamate) via reduction of hydrogen peroxide at +50 mV versus Ag/AgCl in the low micromolar range. Response times were 1-2 min. Finally, the glucose, ethanol and lactate electrochemical biosensors were used to analyse complex food matrices--must, wine and yoghurt. Data obtained by the single standard addition method were compared with a spectrophotometric reference method and showed good correlation, indicating that the electrodes are suitable for food analysis. |
id |
RCAP_8e4a06f6138b9a64605272d2d93ccf5a |
---|---|
oai_identifier_str |
oai:estudogeral.uc.pt:10316/5095 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Carbon film electrodes for oxidase-based enzyme sensors in food analysisOxidase enzymesCarbon film electrodesPrussian Blue mediatorFood analysisCarbon film resistor electrodes have been evaluated as transducers for the development of multiple oxidase-based enzyme electrode biosensors. The resistor electrodes were first modified with Prussian Blue (PB) and then covered by a layer of covalently immobilized enzyme. Electrochemical impedance spectroscopy was used to characterize the interfacial behaviour of the Prussian Blue modified and enzyme electrodes; the spectra demonstrated that the access of the substrates is essentially unaltered by application of the enzyme layer. These enzyme electrodes were used to detect the substrate of the oxidase (glucose, ethanol, lactate, glutamate) via reduction of hydrogen peroxide at +50 mV versus Ag/AgCl in the low micromolar range. Response times were 1-2 min. Finally, the glucose, ethanol and lactate electrochemical biosensors were used to analyse complex food matrices--must, wine and yoghurt. Data obtained by the single standard addition method were compared with a spectrophotometric reference method and showed good correlation, indicating that the electrodes are suitable for food analysis.http://www.sciencedirect.com/science/article/B6THP-4GNTFGP-2/1/087dc22044a84cfb2a1badf317934bca2005info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5095http://hdl.handle.net/10316/5095https://doi.org/10.1016/j.talanta.2005.06.017engTalanta. 68:2 (2005) 171-178Luca, S. DeFlorescu, M.Ghica, M. E.Lupu, A.Palleschi, G.Brett, C. M. A.Compagnone, D.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-05-25T01:32:15Zoai:estudogeral.uc.pt:10316/5095Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:37.831177Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Carbon film electrodes for oxidase-based enzyme sensors in food analysis |
title |
Carbon film electrodes for oxidase-based enzyme sensors in food analysis |
spellingShingle |
Carbon film electrodes for oxidase-based enzyme sensors in food analysis Luca, S. De Oxidase enzymes Carbon film electrodes Prussian Blue mediator Food analysis |
title_short |
Carbon film electrodes for oxidase-based enzyme sensors in food analysis |
title_full |
Carbon film electrodes for oxidase-based enzyme sensors in food analysis |
title_fullStr |
Carbon film electrodes for oxidase-based enzyme sensors in food analysis |
title_full_unstemmed |
Carbon film electrodes for oxidase-based enzyme sensors in food analysis |
title_sort |
Carbon film electrodes for oxidase-based enzyme sensors in food analysis |
author |
Luca, S. De |
author_facet |
Luca, S. De Florescu, M. Ghica, M. E. Lupu, A. Palleschi, G. Brett, C. M. A. Compagnone, D. |
author_role |
author |
author2 |
Florescu, M. Ghica, M. E. Lupu, A. Palleschi, G. Brett, C. M. A. Compagnone, D. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Luca, S. De Florescu, M. Ghica, M. E. Lupu, A. Palleschi, G. Brett, C. M. A. Compagnone, D. |
dc.subject.por.fl_str_mv |
Oxidase enzymes Carbon film electrodes Prussian Blue mediator Food analysis |
topic |
Oxidase enzymes Carbon film electrodes Prussian Blue mediator Food analysis |
description |
Carbon film resistor electrodes have been evaluated as transducers for the development of multiple oxidase-based enzyme electrode biosensors. The resistor electrodes were first modified with Prussian Blue (PB) and then covered by a layer of covalently immobilized enzyme. Electrochemical impedance spectroscopy was used to characterize the interfacial behaviour of the Prussian Blue modified and enzyme electrodes; the spectra demonstrated that the access of the substrates is essentially unaltered by application of the enzyme layer. These enzyme electrodes were used to detect the substrate of the oxidase (glucose, ethanol, lactate, glutamate) via reduction of hydrogen peroxide at +50 mV versus Ag/AgCl in the low micromolar range. Response times were 1-2 min. Finally, the glucose, ethanol and lactate electrochemical biosensors were used to analyse complex food matrices--must, wine and yoghurt. Data obtained by the single standard addition method were compared with a spectrophotometric reference method and showed good correlation, indicating that the electrodes are suitable for food analysis. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5095 http://hdl.handle.net/10316/5095 https://doi.org/10.1016/j.talanta.2005.06.017 |
url |
http://hdl.handle.net/10316/5095 https://doi.org/10.1016/j.talanta.2005.06.017 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Talanta. 68:2 (2005) 171-178 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133907377979392 |