V-ATPase proton pumping activity is required for adult zebrafish appendage regeneration

Detalhes bibliográficos
Autor(a) principal: Monteiro, Joana
Data de Publicação: 2014
Outros Autores: Aires, Rita, Becker, Jörg D., Jacinto, António, Certal, Ana C., Rodríguez-León, Joaquín
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/145291
Resumo: JJM was supported by a fellowship (SFRH/BD/45131/2008) from Fundacao para a Ciencia e Tecnologia (FCT), Portugal. ACC was supported by a postdocoral fellowship (SFRH/BPD/29957/2006) from Fundacao para a Ciencia e Tecnologia (FCT), Portugal. This work was supported by the following grants: european FP6-Cells into Organs, FCT (Grant PTDC/BIA-BCM/100867/2008 and POCTI-ISFL-4-664) and Ministerio de Ciencia e Innovacion, Spain (Subprograma Ramon y Cajal, reference RYC-2008-02753 and grant BFU2009-12279). JRL is supported by the Subprogram Ramon y Cajal from Ministerio de Ciencia e Innovacion, Spain (RYC-2008-02753). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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spelling V-ATPase proton pumping activity is required for adult zebrafish appendage regenerationGeneralJJM was supported by a fellowship (SFRH/BD/45131/2008) from Fundacao para a Ciencia e Tecnologia (FCT), Portugal. ACC was supported by a postdocoral fellowship (SFRH/BPD/29957/2006) from Fundacao para a Ciencia e Tecnologia (FCT), Portugal. This work was supported by the following grants: european FP6-Cells into Organs, FCT (Grant PTDC/BIA-BCM/100867/2008 and POCTI-ISFL-4-664) and Ministerio de Ciencia e Innovacion, Spain (Subprograma Ramon y Cajal, reference RYC-2008-02753 and grant BFU2009-12279). JRL is supported by the Subprogram Ramon y Cajal from Ministerio de Ciencia e Innovacion, Spain (RYC-2008-02753). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.The activity of ion channels and transporters generates ion-specific fluxes that encode electrical and/or chemical signals with biological significance. Even though it is long known that some of those signals are crucial for regeneration, only in recent years the corresponding molecular sources started to be identified using mainly invertebrate or larval vertebrate models. We used adult zebrafish caudal fin as a model to investigate which and how ion transporters affect regeneration in an adult vertebrate model. Through the combined use of biophysical and molecular approaches, we show that V-ATPase activity contributes to a regeneration-specific H+ ef'flux. The onset and intensity of both V-ATPase expression and H+ efflux correlate with the different regeneration rate along the proximal-distal axis. Moreover, we show that V-ATPase inhibition impairs regeneration in adult vertebrate. Notably, the activity of this H+ pump is necessary for aldh1a2 and mkp3 expression, blastema cell proliferation and fin innervation. To the best of our knowledge, this is the first report on the role of V-ATPase during adult vertebrate regeneration.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNMonteiro, JoanaAires, RitaBecker, Jörg D.Jacinto, AntónioCertal, Ana C.Rodríguez-León, Joaquín2022-11-07T22:11:12Z2014-03-262014-03-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/145291eng1932-6203PURE: 47613575https://doi.org/10.1371/journal.pone.0092594info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:25:29Zoai:run.unl.pt:10362/145291Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:51:59.696942Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv V-ATPase proton pumping activity is required for adult zebrafish appendage regeneration
title V-ATPase proton pumping activity is required for adult zebrafish appendage regeneration
spellingShingle V-ATPase proton pumping activity is required for adult zebrafish appendage regeneration
Monteiro, Joana
General
title_short V-ATPase proton pumping activity is required for adult zebrafish appendage regeneration
title_full V-ATPase proton pumping activity is required for adult zebrafish appendage regeneration
title_fullStr V-ATPase proton pumping activity is required for adult zebrafish appendage regeneration
title_full_unstemmed V-ATPase proton pumping activity is required for adult zebrafish appendage regeneration
title_sort V-ATPase proton pumping activity is required for adult zebrafish appendage regeneration
author Monteiro, Joana
author_facet Monteiro, Joana
Aires, Rita
Becker, Jörg D.
Jacinto, António
Certal, Ana C.
Rodríguez-León, Joaquín
author_role author
author2 Aires, Rita
Becker, Jörg D.
Jacinto, António
Certal, Ana C.
Rodríguez-León, Joaquín
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Monteiro, Joana
Aires, Rita
Becker, Jörg D.
Jacinto, António
Certal, Ana C.
Rodríguez-León, Joaquín
dc.subject.por.fl_str_mv General
topic General
description JJM was supported by a fellowship (SFRH/BD/45131/2008) from Fundacao para a Ciencia e Tecnologia (FCT), Portugal. ACC was supported by a postdocoral fellowship (SFRH/BPD/29957/2006) from Fundacao para a Ciencia e Tecnologia (FCT), Portugal. This work was supported by the following grants: european FP6-Cells into Organs, FCT (Grant PTDC/BIA-BCM/100867/2008 and POCTI-ISFL-4-664) and Ministerio de Ciencia e Innovacion, Spain (Subprograma Ramon y Cajal, reference RYC-2008-02753 and grant BFU2009-12279). JRL is supported by the Subprogram Ramon y Cajal from Ministerio de Ciencia e Innovacion, Spain (RYC-2008-02753). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
publishDate 2014
dc.date.none.fl_str_mv 2014-03-26
2014-03-26T00:00:00Z
2022-11-07T22:11:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/145291
url http://hdl.handle.net/10362/145291
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-6203
PURE: 47613575
https://doi.org/10.1371/journal.pone.0092594
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
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instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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