Biosensor for label-free DNA quantification based on functionalized LPGs

Detalhes bibliográficos
Autor(a) principal: Goncalves,HMR
Data de Publicação: 2016
Outros Autores: Moreira,L, Pereira,L, Pedro Jorge, Gouveia,C, Martins Lopes,P, José Ramiro Fernandes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://repositorio.inesctec.pt/handle/123456789/7343
http://dx.doi.org/10.1016/j.bios.2015.10.001
Resumo: A label-free fiber optic biosensor based on a long period grating (LPG) and a basic optical interrogation scheme using off the shelf components is used for the detection of in-situ DNA hybridization. A new methodology is proposed for the determination of the spectral position of the LPG mode resonance. The experimental limit of detection obtained for the DNA was 62 +/- 2 nM and the limit of quantification was 209 +/- 7 nM. The sample specificity was experimentally demonstrated using DNA targets with different base mismatches relatively to the probe and was found that the system has a single base mismatch selectivity.
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spelling Biosensor for label-free DNA quantification based on functionalized LPGsA label-free fiber optic biosensor based on a long period grating (LPG) and a basic optical interrogation scheme using off the shelf components is used for the detection of in-situ DNA hybridization. A new methodology is proposed for the determination of the spectral position of the LPG mode resonance. The experimental limit of detection obtained for the DNA was 62 +/- 2 nM and the limit of quantification was 209 +/- 7 nM. The sample specificity was experimentally demonstrated using DNA targets with different base mismatches relatively to the probe and was found that the system has a single base mismatch selectivity.2018-01-24T15:35:15Z2016-01-01T00:00:00Z2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://repositorio.inesctec.pt/handle/123456789/7343http://dx.doi.org/10.1016/j.bios.2015.10.001engGoncalves,HMRMoreira,LPereira,LPedro JorgeGouveia,CMartins Lopes,PJosé Ramiro Fernandesinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-05-15T10:20:12Zoai:repositorio.inesctec.pt:123456789/7343Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:52:49.129320Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biosensor for label-free DNA quantification based on functionalized LPGs
title Biosensor for label-free DNA quantification based on functionalized LPGs
spellingShingle Biosensor for label-free DNA quantification based on functionalized LPGs
Goncalves,HMR
title_short Biosensor for label-free DNA quantification based on functionalized LPGs
title_full Biosensor for label-free DNA quantification based on functionalized LPGs
title_fullStr Biosensor for label-free DNA quantification based on functionalized LPGs
title_full_unstemmed Biosensor for label-free DNA quantification based on functionalized LPGs
title_sort Biosensor for label-free DNA quantification based on functionalized LPGs
author Goncalves,HMR
author_facet Goncalves,HMR
Moreira,L
Pereira,L
Pedro Jorge
Gouveia,C
Martins Lopes,P
José Ramiro Fernandes
author_role author
author2 Moreira,L
Pereira,L
Pedro Jorge
Gouveia,C
Martins Lopes,P
José Ramiro Fernandes
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Goncalves,HMR
Moreira,L
Pereira,L
Pedro Jorge
Gouveia,C
Martins Lopes,P
José Ramiro Fernandes
description A label-free fiber optic biosensor based on a long period grating (LPG) and a basic optical interrogation scheme using off the shelf components is used for the detection of in-situ DNA hybridization. A new methodology is proposed for the determination of the spectral position of the LPG mode resonance. The experimental limit of detection obtained for the DNA was 62 +/- 2 nM and the limit of quantification was 209 +/- 7 nM. The sample specificity was experimentally demonstrated using DNA targets with different base mismatches relatively to the probe and was found that the system has a single base mismatch selectivity.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01T00:00:00Z
2016
2018-01-24T15:35:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.inesctec.pt/handle/123456789/7343
http://dx.doi.org/10.1016/j.bios.2015.10.001
url http://repositorio.inesctec.pt/handle/123456789/7343
http://dx.doi.org/10.1016/j.bios.2015.10.001
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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