Acetylcholinesterase Biosensor Based on Functionalized Renewable Carbon Platform for Detection of Carbaryl in Food
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/bios12070486 http://hdl.handle.net/11449/240590 |
Resumo: | Enzymatic electrochemical biosensors play an important role in the agri-food sector due to the need to develop sustainable, low-cost, and easy-to-use analytical devices. Such biosensors can be used to monitor pathogens, endocrine disruptors, and pesticides, such as carbaryl, widely used in many crops. The use of renewable carbon (RC) sources, provided from biomass pyrolysis has been often applied in the fabrication of such sensors. This material is a great candidate for biosensor fabrication due to the presence of surface functional groups, porosity, and moderate surface area. This work describes the functionalization of RC material through an acid treatment with a sulfonitric solution HNO3/H2SO4 (1:3) and the resulting material was characterized by scanning electron microscopy. The obtained RC functionalized (RCF) and the acetylcholinesterase enzyme (AChE) were applied in the construction of the electrochemical biosensor on glassy carbon (GC) electrode and used to detect carbaryl in apple samples. The GC/RCF/AChE biosensor was able to detect the carbaryl pesticide from 5.0 to 30.0 nmol L−1, displaying a LOD of 4.5 nmol L−1. The detection of carbaryl in apple samples presented recoveries between 102.5 to 118.6% through the standard addition method. The proposed biosensor is a promising renewable tool for food safety. |
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Acetylcholinesterase Biosensor Based on Functionalized Renewable Carbon Platform for Detection of Carbaryl in Foodacetylcholinesterase enzymecarbarylelectrochemical biosensorfood safetyrenewable carbonEnzymatic electrochemical biosensors play an important role in the agri-food sector due to the need to develop sustainable, low-cost, and easy-to-use analytical devices. Such biosensors can be used to monitor pathogens, endocrine disruptors, and pesticides, such as carbaryl, widely used in many crops. The use of renewable carbon (RC) sources, provided from biomass pyrolysis has been often applied in the fabrication of such sensors. This material is a great candidate for biosensor fabrication due to the presence of surface functional groups, porosity, and moderate surface area. This work describes the functionalization of RC material through an acid treatment with a sulfonitric solution HNO3/H2SO4 (1:3) and the resulting material was characterized by scanning electron microscopy. The obtained RC functionalized (RCF) and the acetylcholinesterase enzyme (AChE) were applied in the construction of the electrochemical biosensor on glassy carbon (GC) electrode and used to detect carbaryl in apple samples. The GC/RCF/AChE biosensor was able to detect the carbaryl pesticide from 5.0 to 30.0 nmol L−1, displaying a LOD of 4.5 nmol L−1. The detection of carbaryl in apple samples presented recoveries between 102.5 to 118.6% through the standard addition method. The proposed biosensor is a promising renewable tool for food safety.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)School of Agriculture São Paulo State University (UNESP)Instituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES) Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas (UNTRM)Instituto de Investigación en Ganadería y Biotecnología (IGBI) Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas (UNTRM)Facultad de Ciencias de la Salud (FACISA) Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas (UNTRM)School of Agriculture São Paulo State University (UNESP)FAPESP: 2017/24274-3FAPESP: 2019/19578-9Universidade Estadual Paulista (UNESP)Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas (UNTRM)Nunes, Erik W. [UNESP]Silva, Martin K. L. [UNESP]Rascón, JesúsLeiva-Tafur, DamarisLapa, Rainer M. L.Cesarino, Ivana [UNESP]2023-03-01T20:24:08Z2023-03-01T20:24:08Z2022-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/bios12070486Biosensors, v. 12, n. 7, 2022.2079-6374http://hdl.handle.net/11449/24059010.3390/bios120704862-s2.0-85135369322Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiosensorsinfo:eu-repo/semantics/openAccess2023-03-01T20:24:08Zoai:repositorio.unesp.br:11449/240590Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T20:24:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Acetylcholinesterase Biosensor Based on Functionalized Renewable Carbon Platform for Detection of Carbaryl in Food |
title |
Acetylcholinesterase Biosensor Based on Functionalized Renewable Carbon Platform for Detection of Carbaryl in Food |
spellingShingle |
Acetylcholinesterase Biosensor Based on Functionalized Renewable Carbon Platform for Detection of Carbaryl in Food Nunes, Erik W. [UNESP] acetylcholinesterase enzyme carbaryl electrochemical biosensor food safety renewable carbon |
title_short |
Acetylcholinesterase Biosensor Based on Functionalized Renewable Carbon Platform for Detection of Carbaryl in Food |
title_full |
Acetylcholinesterase Biosensor Based on Functionalized Renewable Carbon Platform for Detection of Carbaryl in Food |
title_fullStr |
Acetylcholinesterase Biosensor Based on Functionalized Renewable Carbon Platform for Detection of Carbaryl in Food |
title_full_unstemmed |
Acetylcholinesterase Biosensor Based on Functionalized Renewable Carbon Platform for Detection of Carbaryl in Food |
title_sort |
Acetylcholinesterase Biosensor Based on Functionalized Renewable Carbon Platform for Detection of Carbaryl in Food |
author |
Nunes, Erik W. [UNESP] |
author_facet |
Nunes, Erik W. [UNESP] Silva, Martin K. L. [UNESP] Rascón, Jesús Leiva-Tafur, Damaris Lapa, Rainer M. L. Cesarino, Ivana [UNESP] |
author_role |
author |
author2 |
Silva, Martin K. L. [UNESP] Rascón, Jesús Leiva-Tafur, Damaris Lapa, Rainer M. L. Cesarino, Ivana [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas (UNTRM) |
dc.contributor.author.fl_str_mv |
Nunes, Erik W. [UNESP] Silva, Martin K. L. [UNESP] Rascón, Jesús Leiva-Tafur, Damaris Lapa, Rainer M. L. Cesarino, Ivana [UNESP] |
dc.subject.por.fl_str_mv |
acetylcholinesterase enzyme carbaryl electrochemical biosensor food safety renewable carbon |
topic |
acetylcholinesterase enzyme carbaryl electrochemical biosensor food safety renewable carbon |
description |
Enzymatic electrochemical biosensors play an important role in the agri-food sector due to the need to develop sustainable, low-cost, and easy-to-use analytical devices. Such biosensors can be used to monitor pathogens, endocrine disruptors, and pesticides, such as carbaryl, widely used in many crops. The use of renewable carbon (RC) sources, provided from biomass pyrolysis has been often applied in the fabrication of such sensors. This material is a great candidate for biosensor fabrication due to the presence of surface functional groups, porosity, and moderate surface area. This work describes the functionalization of RC material through an acid treatment with a sulfonitric solution HNO3/H2SO4 (1:3) and the resulting material was characterized by scanning electron microscopy. The obtained RC functionalized (RCF) and the acetylcholinesterase enzyme (AChE) were applied in the construction of the electrochemical biosensor on glassy carbon (GC) electrode and used to detect carbaryl in apple samples. The GC/RCF/AChE biosensor was able to detect the carbaryl pesticide from 5.0 to 30.0 nmol L−1, displaying a LOD of 4.5 nmol L−1. The detection of carbaryl in apple samples presented recoveries between 102.5 to 118.6% through the standard addition method. The proposed biosensor is a promising renewable tool for food safety. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-01 2023-03-01T20:24:08Z 2023-03-01T20:24:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/bios12070486 Biosensors, v. 12, n. 7, 2022. 2079-6374 http://hdl.handle.net/11449/240590 10.3390/bios12070486 2-s2.0-85135369322 |
url |
http://dx.doi.org/10.3390/bios12070486 http://hdl.handle.net/11449/240590 |
identifier_str_mv |
Biosensors, v. 12, n. 7, 2022. 2079-6374 10.3390/bios12070486 2-s2.0-85135369322 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biosensors |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965491625721856 |