Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant biomasses

Detalhes bibliográficos
Autor(a) principal: Gavande, Parmeshwar Vitthal
Data de Publicação: 2022
Outros Autores: Nath, Priyanka, Kumar, Krishan, Ahmed, Nazneen, Fontes, Carlos M.G.A., Goyal, Arun
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/25046
Resumo: Research Areas: Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
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spelling Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant biomassesMultifunctionalProcessiveEndoglucanaseResearch Areas: Biochemistry & Molecular Biology ; Chemistry ; Polymer ScienceGene encoding endoglucanase, RfGH5_4 from R. flavefaciens FD-1 v3 was cloned, expressed in Escherichia coli BL21(DE3) cells and purified. RfGH5_4 showed molecular size 41 kDa and maximum activity at pH 5.5 and 55 ◦C. It was stable between pH 5.0–8.0, retaining 85% activity and between 5 ◦C–45 ◦C, retaining 75% activity, after 60 min. RfGH5_4 displayed maximum activity (U/mg) against barley β-D-glucan (665) followed by carboxymethyl cellulose (450), xyloglucan (343), konjac glucomannan (285), phosphoric acid swollen cellulose (86), beechwood xylan (21.7) and carob galactomannan (16), thereby displaying the multi-functionality. Catalytic efficiency (mL.mg− 1 s − 1 ) of RfGH5_4 against carboxymethyl cellulose (146) and konjac glucomannan (529) was significantly high. TLC and MALDI-TOF-MS analyses of RfGH5_4 treated hydrolysates of cellulosic and hemicellulosic polysaccharides displayed oligosaccharides of degree of polymerization (DP) between DP2-DP11. TLC, HPLC and Processivity-Index analyses revealed RfGH5_4 to be a processive endoglucanase as initially, for 30 min it hydrolysed cellulose to cellotetraose followed by persistent release of cellotriose and cellobiose. RfGH5_4 yielded sufficiently high Total Reducing Sugar (TRS, mg/g) from saccharification of alkali pre-treated sorghum (72), finger millet (62), sugarcane bagasse (38) and cotton (27) in a 48 h saccharification reaction. Thus, RfGH5_4 can be considered as a potential endoglucanase for renewable energy applications.ELSEVIERRepositório da Universidade de LisboaGavande, Parmeshwar VitthalNath, PriyankaKumar, KrishanAhmed, NazneenFontes, Carlos M.G.A.Goyal, Arun2022-07-29T11:11:18Z2022-06-012022-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/25046engGavande PV, Nath P, Kumar K, Ahmed N, Fontes CMGA, Goyal A. 2022. Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant biomasses. International Journal of Biological Macromolecules, 209:801-813. DOI: https://doi.org/10.1016/j.ijbiomac.2022.04.0590141-8130https://doi.org/10.1016/j.ijbiomac.2022.04.0591879-0003info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:54:40Zoai:www.repository.utl.pt:10400.5/25046Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:08:59.927699Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant biomasses
title Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant biomasses
spellingShingle Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant biomasses
Gavande, Parmeshwar Vitthal
Multifunctional
Processive
Endoglucanase
title_short Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant biomasses
title_full Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant biomasses
title_fullStr Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant biomasses
title_full_unstemmed Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant biomasses
title_sort Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant biomasses
author Gavande, Parmeshwar Vitthal
author_facet Gavande, Parmeshwar Vitthal
Nath, Priyanka
Kumar, Krishan
Ahmed, Nazneen
Fontes, Carlos M.G.A.
Goyal, Arun
author_role author
author2 Nath, Priyanka
Kumar, Krishan
Ahmed, Nazneen
Fontes, Carlos M.G.A.
Goyal, Arun
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Gavande, Parmeshwar Vitthal
Nath, Priyanka
Kumar, Krishan
Ahmed, Nazneen
Fontes, Carlos M.G.A.
Goyal, Arun
dc.subject.por.fl_str_mv Multifunctional
Processive
Endoglucanase
topic Multifunctional
Processive
Endoglucanase
description Research Areas: Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
publishDate 2022
dc.date.none.fl_str_mv 2022-07-29T11:11:18Z
2022-06-01
2022-06-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/25046
url http://hdl.handle.net/10400.5/25046
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gavande PV, Nath P, Kumar K, Ahmed N, Fontes CMGA, Goyal A. 2022. Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant biomasses. International Journal of Biological Macromolecules, 209:801-813. DOI: https://doi.org/10.1016/j.ijbiomac.2022.04.059
0141-8130
https://doi.org/10.1016/j.ijbiomac.2022.04.059
1879-0003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ELSEVIER
publisher.none.fl_str_mv ELSEVIER
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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