Integrated use of residues from olive mill and winery for lipase production by solid state fermentation with Aspergillus sp

Detalhes bibliográficos
Autor(a) principal: Salgado, José Manuel
Data de Publicação: 2014
Outros Autores: Abrunhosa, Luís, Venâncio, Armando, Domínguez, José Manuel, Belo, Isabel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/31473
Resumo: Two phase olive mill waste (TPOMW) is presently the major waste produced by the olive mill industry. This waste has potential to be used as substrate for solid state fermentation (SSF) despite of its high concentration of phenolic compounds and low nitrogen content. In this work, it is demonstrated that mixtures of TPOMW with winery wastes support the production of lipase by Aspergillus spp. By agar plate screening, Aspergillus niger MUM 03.58, Aspergillus ibericus MUM 03.49 and Aspergillus uvarum MUM 08.01 were chosen for lipase production by SSF. Plackett-Burman experimental design was employed to evaluate the effect of substrate composition and time on lipase production. The highest amounts of lipase were produced by A. ibericus on a mixture of TPOMW, urea and exhausted grape mark (EGM). Urea was found to be the most influent factor for the lipase production. Further optimization of lipase production by A. ibericus using a full factorial design (32) conducted to optimal conditions of substrate composition (0.073 g urea/g and 25% of EGM) achieving 18.67 U/g of lipolytic activity.
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spelling Integrated use of residues from olive mill and winery for lipase production by solid state fermentation with Aspergillus spOlive mill wastesWinery wastesPlackett–burmanSolid state fermentationAspergillus spScience & TechnologyTwo phase olive mill waste (TPOMW) is presently the major waste produced by the olive mill industry. This waste has potential to be used as substrate for solid state fermentation (SSF) despite of its high concentration of phenolic compounds and low nitrogen content. In this work, it is demonstrated that mixtures of TPOMW with winery wastes support the production of lipase by Aspergillus spp. By agar plate screening, Aspergillus niger MUM 03.58, Aspergillus ibericus MUM 03.49 and Aspergillus uvarum MUM 08.01 were chosen for lipase production by SSF. Plackett-Burman experimental design was employed to evaluate the effect of substrate composition and time on lipase production. The highest amounts of lipase were produced by A. ibericus on a mixture of TPOMW, urea and exhausted grape mark (EGM). Urea was found to be the most influent factor for the lipase production. Further optimization of lipase production by A. ibericus using a full factorial design (32) conducted to optimal conditions of substrate composition (0.073 g urea/g and 25% of EGM) achieving 18.67 U/g of lipolytic activity.Jose Manuel Salgado is grateful for Postdoctoral fellowship (EX-2010-0402) of Education Ministry of Spanish Government. Luis Abrunhosa was supported by the grant SFRH/BPD/43922/2008 from Fundacao para a Ciencia e Tecnologia-FCT, Portugal. Authors thank Fundacao para a Ciencia e a Tecnologia (FCT) for financial support through the project FCT Pest-OE/EQB/LA0023/2011.SpringerUniversidade do MinhoSalgado, José ManuelAbrunhosa, LuísVenâncio, ArmandoDomínguez, José ManuelBelo, Isabel20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31473engSalgado, José Manuel; Abrunhosa, Luís; Venâncio, Armando; Domínguez, J. M.; Belo, Isabel, Integrated use of residues from olive mill and winery for lipase production by solid state fermentation with Aspergillus sp.. Applied Biochemistry and Biotechnology, 172(4), 1832-1845, 20140273-22890273-228910.1007/s12010-013-0613-424276916http://link.springer.com/journal/12010info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:10:51Zoai:repositorium.sdum.uminho.pt:1822/31473Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:02:32.756969Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Integrated use of residues from olive mill and winery for lipase production by solid state fermentation with Aspergillus sp
title Integrated use of residues from olive mill and winery for lipase production by solid state fermentation with Aspergillus sp
spellingShingle Integrated use of residues from olive mill and winery for lipase production by solid state fermentation with Aspergillus sp
Salgado, José Manuel
Olive mill wastes
Winery wastes
Plackett–burman
Solid state fermentation
Aspergillus sp
Science & Technology
title_short Integrated use of residues from olive mill and winery for lipase production by solid state fermentation with Aspergillus sp
title_full Integrated use of residues from olive mill and winery for lipase production by solid state fermentation with Aspergillus sp
title_fullStr Integrated use of residues from olive mill and winery for lipase production by solid state fermentation with Aspergillus sp
title_full_unstemmed Integrated use of residues from olive mill and winery for lipase production by solid state fermentation with Aspergillus sp
title_sort Integrated use of residues from olive mill and winery for lipase production by solid state fermentation with Aspergillus sp
author Salgado, José Manuel
author_facet Salgado, José Manuel
Abrunhosa, Luís
Venâncio, Armando
Domínguez, José Manuel
Belo, Isabel
author_role author
author2 Abrunhosa, Luís
Venâncio, Armando
Domínguez, José Manuel
Belo, Isabel
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Salgado, José Manuel
Abrunhosa, Luís
Venâncio, Armando
Domínguez, José Manuel
Belo, Isabel
dc.subject.por.fl_str_mv Olive mill wastes
Winery wastes
Plackett–burman
Solid state fermentation
Aspergillus sp
Science & Technology
topic Olive mill wastes
Winery wastes
Plackett–burman
Solid state fermentation
Aspergillus sp
Science & Technology
description Two phase olive mill waste (TPOMW) is presently the major waste produced by the olive mill industry. This waste has potential to be used as substrate for solid state fermentation (SSF) despite of its high concentration of phenolic compounds and low nitrogen content. In this work, it is demonstrated that mixtures of TPOMW with winery wastes support the production of lipase by Aspergillus spp. By agar plate screening, Aspergillus niger MUM 03.58, Aspergillus ibericus MUM 03.49 and Aspergillus uvarum MUM 08.01 were chosen for lipase production by SSF. Plackett-Burman experimental design was employed to evaluate the effect of substrate composition and time on lipase production. The highest amounts of lipase were produced by A. ibericus on a mixture of TPOMW, urea and exhausted grape mark (EGM). Urea was found to be the most influent factor for the lipase production. Further optimization of lipase production by A. ibericus using a full factorial design (32) conducted to optimal conditions of substrate composition (0.073 g urea/g and 25% of EGM) achieving 18.67 U/g of lipolytic activity.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/31473
url http://hdl.handle.net/1822/31473
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Salgado, José Manuel; Abrunhosa, Luís; Venâncio, Armando; Domínguez, J. M.; Belo, Isabel, Integrated use of residues from olive mill and winery for lipase production by solid state fermentation with Aspergillus sp.. Applied Biochemistry and Biotechnology, 172(4), 1832-1845, 2014
0273-2289
0273-2289
10.1007/s12010-013-0613-4
24276916
http://link.springer.com/journal/12010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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