Forensic evidence from the biochemical composition of human fingerprints

Detalhes bibliográficos
Autor(a) principal: Morgado, Bárbara Filipa Alves
Data de Publicação: 2020
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/47933
Resumo: Tese de mestrado, Bioquímica (Bioquímica) Universidade de Lisboa, Faculdade de Ciências, 2021
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spelling Forensic evidence from the biochemical composition of human fingerprintsForenseImpressão digitalEspectrometria de massaEspectrometria de massa de ressonância ciclotrónica de ião com transformada de fourierCompostos endógenosCompostos exógenosLípidosMetabolómicaTeses de mestrado - 2021Departamento de Química e BioquímicaTese de mestrado, Bioquímica (Bioquímica) Universidade de Lisboa, Faculdade de Ciências, 2021Throughout the years, fingerprints have been used in criminal investigations to place a suspect at a crime scene. However, this method depends on the integrity of the fingermark, on the method used for its recovery and if the respective fingerprint is listed or not on the National/International Fingerprint Database. The ridge pattern does not use all available information, most notably, the chemical signature that is left behind. So, it could be extremely beneficial to invest in techniques that can retrieve basic donor information in fingerprints’ chemical composition. Until now, human fingerprints have been mainly analysed by low resolution mass spectrometry methods. There is one particular area of mass spectrometry that has led the study of fingerprint’s chemical composition and it is Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS). With this technique, not only a fingerprint’s image is obtained, which may be used in a biometric identification, but it’s also possible to profile the fingerprint’s chemical composition. However, this type of approach does not allow a bulk analysis of its chemical composition. With that in mind, this work coupled Electrospray Ionization (ESI) with a high resolution mass spectrometry method such as FT-ICR MS, to reveal the chemical composition of human fingerprints. The compositional space of 10 fingerprints was analysed combining accurate mass measurements, database search and molecular formula determination, which was explored trough Principal Component Analysis (PCA), Kruskal-Wallis tests and other tools. From the thirty eight compounds commonly found in all fingerprints that were analysed, twenty (mainly lipids), such as palmitic acid, oleic acid, stearic acid and linoleic acid, were present with similar intensities. This was the first step towards the definition of a chemical background for the composition of human fingerprints in relation to which everything else can be found. Understanding the source of these compounds will be important for the interpretation of their possible roles on fingertips and potential uses in forensic and other contexts. Despite the main focus being endogenous compounds, we also found several exogenous com-pounds, for example, pantothenol which is present in personal care products, mainly shampoos. It was possible to verify that in fact this compound was part of the composition of the shampoo used by the volunteer in question. Therefore, the use of ESI together with FT-ICR MS has proven to be a promising analytical method for studying the chemical signature of fingerprints due to its unmatched sensitivity for detecting any type of molecules, extreme sensitivity, requiring only minute amounts of sample and the fact that it is an high-throughput method. Thus, it can be used not only from a forensic point of view, but also in other contexts, namely, clinical.Cordeiro, Carlos, 1966-Repositório da Universidade de LisboaMorgado, Bárbara Filipa Alves2021-12-27T01:30:21Z202120202021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10451/47933TID:202933156enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:51:07Zoai:repositorio.ul.pt:10451/47933Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:59:51.899109Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Forensic evidence from the biochemical composition of human fingerprints
title Forensic evidence from the biochemical composition of human fingerprints
spellingShingle Forensic evidence from the biochemical composition of human fingerprints
Morgado, Bárbara Filipa Alves
Forense
Impressão digital
Espectrometria de massa
Espectrometria de massa de ressonância ciclotrónica de ião com transformada de fourier
Compostos endógenos
Compostos exógenos
Lípidos
Metabolómica
Teses de mestrado - 2021
Departamento de Química e Bioquímica
title_short Forensic evidence from the biochemical composition of human fingerprints
title_full Forensic evidence from the biochemical composition of human fingerprints
title_fullStr Forensic evidence from the biochemical composition of human fingerprints
title_full_unstemmed Forensic evidence from the biochemical composition of human fingerprints
title_sort Forensic evidence from the biochemical composition of human fingerprints
author Morgado, Bárbara Filipa Alves
author_facet Morgado, Bárbara Filipa Alves
author_role author
dc.contributor.none.fl_str_mv Cordeiro, Carlos, 1966-
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Morgado, Bárbara Filipa Alves
dc.subject.por.fl_str_mv Forense
Impressão digital
Espectrometria de massa
Espectrometria de massa de ressonância ciclotrónica de ião com transformada de fourier
Compostos endógenos
Compostos exógenos
Lípidos
Metabolómica
Teses de mestrado - 2021
Departamento de Química e Bioquímica
topic Forense
Impressão digital
Espectrometria de massa
Espectrometria de massa de ressonância ciclotrónica de ião com transformada de fourier
Compostos endógenos
Compostos exógenos
Lípidos
Metabolómica
Teses de mestrado - 2021
Departamento de Química e Bioquímica
description Tese de mestrado, Bioquímica (Bioquímica) Universidade de Lisboa, Faculdade de Ciências, 2021
publishDate 2020
dc.date.none.fl_str_mv 2020
2021-12-27T01:30:21Z
2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/47933
TID:202933156
url http://hdl.handle.net/10451/47933
identifier_str_mv TID:202933156
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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