Participation of Candida albicans transcription factor Rlm1 in cell wall biogenesis and virulence
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/32074 |
Resumo: | Candida albicans cell wall is important for growth and interaction with the environment. RLM1 is one of the putative transcription factors involved in the cell wall integrity pathway, which plays an important role in the maintenance of the cell wall integrity. In this work we investigated the involvement of RLM1 in the cell wall biogenesis and in virulence. Newly constructed C. albicans Δ/Δrlm1 mutants showed typical cell wall weakening phenotypes, such as hypersensitivity to Congo Red, Calcofluor White, and caspofungin (phenotype reverted in the presence of sorbitol), confirming the involvement of RLM1 in the cell wall integrity. Additionally, the cell wall of C. albicans Δ/Δrlm1 showed a significant increase in chitin (213%) and reduction in mannans (60%), in comparison with the wild-type, results that are consistent with cell wall remodelling. Microarray analysis in the absence of any stress showed that deletion of RLM1 in C. albicans significantly down-regulated genes involved in carbohydrate catabolism such as DAK2, GLK4, NHT1 and TPS1, up-regulated genes involved in the utilization of alternative carbon sources, like AGP2, SOU1, SAP6, CIT1 or GAL4, and genes involved in cell adhesion like ECE1, ALS1, ALS3, HWP1 or RBT1. In agreement with the microarray results adhesion assays showed an increased amount of adhering cells and total biomass in the mutant strain, in comparison with the wild-type. C. albicans mutant Δ/Δrlm1 strain was also found to be less virulent than the wild-type and complemented strains in the murine model of disseminated candidiasis. Overall, we showed that in the absence of RLM1 the modifications in the cell wall composition alter yeast interaction with the environment, with consequences in adhesion ability and virulence. The gene expression findings suggest that this gene participates in the cell wall biogenesis, with the mutant rearranging its metabolic pathways to allow the use of alternative carbon sources. |
id |
RCAP_905f6714180e3cee9ada520c747740ac |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/32074 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Participation of Candida albicans transcription factor Rlm1 in cell wall biogenesis and virulenceScience & TechnologyCandida albicans cell wall is important for growth and interaction with the environment. RLM1 is one of the putative transcription factors involved in the cell wall integrity pathway, which plays an important role in the maintenance of the cell wall integrity. In this work we investigated the involvement of RLM1 in the cell wall biogenesis and in virulence. Newly constructed C. albicans Δ/Δrlm1 mutants showed typical cell wall weakening phenotypes, such as hypersensitivity to Congo Red, Calcofluor White, and caspofungin (phenotype reverted in the presence of sorbitol), confirming the involvement of RLM1 in the cell wall integrity. Additionally, the cell wall of C. albicans Δ/Δrlm1 showed a significant increase in chitin (213%) and reduction in mannans (60%), in comparison with the wild-type, results that are consistent with cell wall remodelling. Microarray analysis in the absence of any stress showed that deletion of RLM1 in C. albicans significantly down-regulated genes involved in carbohydrate catabolism such as DAK2, GLK4, NHT1 and TPS1, up-regulated genes involved in the utilization of alternative carbon sources, like AGP2, SOU1, SAP6, CIT1 or GAL4, and genes involved in cell adhesion like ECE1, ALS1, ALS3, HWP1 or RBT1. In agreement with the microarray results adhesion assays showed an increased amount of adhering cells and total biomass in the mutant strain, in comparison with the wild-type. C. albicans mutant Δ/Δrlm1 strain was also found to be less virulent than the wild-type and complemented strains in the murine model of disseminated candidiasis. Overall, we showed that in the absence of RLM1 the modifications in the cell wall composition alter yeast interaction with the environment, with consequences in adhesion ability and virulence. The gene expression findings suggest that this gene participates in the cell wall biogenesis, with the mutant rearranging its metabolic pathways to allow the use of alternative carbon sources.This work was supported by CBMA (Centre of Molecular and Environmental Biology) through the FCT (Fundacao para a Ciencia e Tecnologia) project PEst-C/BIA/UI4050/2011. Yolanda Delgado-Silva was supported by an ALbAN scholarship (No E07D400922PE), and Alexandra Correia by SFRH/BD/31354/2006 fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Public Library of ScienceUniversidade do MinhoSilva, Yolanda Bedsabé DelgadoVaz, Catarina OliveiraPereira, Joana CarvalhoCarneiro, CatarinaNogueira, Eugénia Sofia CostaCorreia, AlexandraCarreto, LauraSilva, Sónia CarinaFaustino, AugustoPais, CéliaOliveira, RuiSampaio, Paula20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/32074engDelgado-Silva, Y.; Vaz, C.; Carvalho-Pereira, J.; Carneiro, C.; Nogueira, E.; Correia, A.; Carreto, L.; Silva, Sónia Carina; Augusto, F.; Pais, C.; Oliveira, R.; Sampaio, P., Participation of Candida albicans transcription factor Rlm1 in cell wall biogenesis and virulence. PLoS One, 9(1), 1-13, 20141932-62031932-620310.1371/journal.pone.008627024466000info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:27:27Zoai:repositorium.sdum.uminho.pt:1822/32074Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:22:03.101105Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Participation of Candida albicans transcription factor Rlm1 in cell wall biogenesis and virulence |
title |
Participation of Candida albicans transcription factor Rlm1 in cell wall biogenesis and virulence |
spellingShingle |
Participation of Candida albicans transcription factor Rlm1 in cell wall biogenesis and virulence Silva, Yolanda Bedsabé Delgado Science & Technology |
title_short |
Participation of Candida albicans transcription factor Rlm1 in cell wall biogenesis and virulence |
title_full |
Participation of Candida albicans transcription factor Rlm1 in cell wall biogenesis and virulence |
title_fullStr |
Participation of Candida albicans transcription factor Rlm1 in cell wall biogenesis and virulence |
title_full_unstemmed |
Participation of Candida albicans transcription factor Rlm1 in cell wall biogenesis and virulence |
title_sort |
Participation of Candida albicans transcription factor Rlm1 in cell wall biogenesis and virulence |
author |
Silva, Yolanda Bedsabé Delgado |
author_facet |
Silva, Yolanda Bedsabé Delgado Vaz, Catarina Oliveira Pereira, Joana Carvalho Carneiro, Catarina Nogueira, Eugénia Sofia Costa Correia, Alexandra Carreto, Laura Silva, Sónia Carina Faustino, Augusto Pais, Célia Oliveira, Rui Sampaio, Paula |
author_role |
author |
author2 |
Vaz, Catarina Oliveira Pereira, Joana Carvalho Carneiro, Catarina Nogueira, Eugénia Sofia Costa Correia, Alexandra Carreto, Laura Silva, Sónia Carina Faustino, Augusto Pais, Célia Oliveira, Rui Sampaio, Paula |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Silva, Yolanda Bedsabé Delgado Vaz, Catarina Oliveira Pereira, Joana Carvalho Carneiro, Catarina Nogueira, Eugénia Sofia Costa Correia, Alexandra Carreto, Laura Silva, Sónia Carina Faustino, Augusto Pais, Célia Oliveira, Rui Sampaio, Paula |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
Candida albicans cell wall is important for growth and interaction with the environment. RLM1 is one of the putative transcription factors involved in the cell wall integrity pathway, which plays an important role in the maintenance of the cell wall integrity. In this work we investigated the involvement of RLM1 in the cell wall biogenesis and in virulence. Newly constructed C. albicans Δ/Δrlm1 mutants showed typical cell wall weakening phenotypes, such as hypersensitivity to Congo Red, Calcofluor White, and caspofungin (phenotype reverted in the presence of sorbitol), confirming the involvement of RLM1 in the cell wall integrity. Additionally, the cell wall of C. albicans Δ/Δrlm1 showed a significant increase in chitin (213%) and reduction in mannans (60%), in comparison with the wild-type, results that are consistent with cell wall remodelling. Microarray analysis in the absence of any stress showed that deletion of RLM1 in C. albicans significantly down-regulated genes involved in carbohydrate catabolism such as DAK2, GLK4, NHT1 and TPS1, up-regulated genes involved in the utilization of alternative carbon sources, like AGP2, SOU1, SAP6, CIT1 or GAL4, and genes involved in cell adhesion like ECE1, ALS1, ALS3, HWP1 or RBT1. In agreement with the microarray results adhesion assays showed an increased amount of adhering cells and total biomass in the mutant strain, in comparison with the wild-type. C. albicans mutant Δ/Δrlm1 strain was also found to be less virulent than the wild-type and complemented strains in the murine model of disseminated candidiasis. Overall, we showed that in the absence of RLM1 the modifications in the cell wall composition alter yeast interaction with the environment, with consequences in adhesion ability and virulence. The gene expression findings suggest that this gene participates in the cell wall biogenesis, with the mutant rearranging its metabolic pathways to allow the use of alternative carbon sources. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/32074 |
url |
http://hdl.handle.net/1822/32074 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Delgado-Silva, Y.; Vaz, C.; Carvalho-Pereira, J.; Carneiro, C.; Nogueira, E.; Correia, A.; Carreto, L.; Silva, Sónia Carina; Augusto, F.; Pais, C.; Oliveira, R.; Sampaio, P., Participation of Candida albicans transcription factor Rlm1 in cell wall biogenesis and virulence. PLoS One, 9(1), 1-13, 2014 1932-6203 1932-6203 10.1371/journal.pone.0086270 24466000 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132689944543232 |