Proteostasis, inflamatory and vasoactive biomarkers in patients with hypertension: effect of exercise training
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/25071 |
Resumo: | Background: Physical exercise is a consensual and well-established strategy to control blood pressure. Nonetheless, its effects on protein homeostasis in individuals with hypertension are not clearly defined. Aims: This study aimed to evaluate the effects of regular exercise on inflammatory, oxidative stress and vasoactive markers in circulation, as well as on protein homeostasis and quality of life in individuals with hypertension. Furthermore, we intended to correlate quality of life with the levels of the above-mentioned biomarkers. Methods: A total of 20 individuals with hypertension were recruited and divided into 2 groups of 10: a group who regularly participated in physical exercise (”EH”, age: 68.3 ± 4.2 years), and an age-matched group without regular exercise practice (“H”, age: 67.7 ± 5.1 years). Proteostasis and vasoactive markers were assessed in plasma using immunoblotting techniques (western blot or slot-blot) and Fourier-transform infrared spectroscopy (FTIR). The levels of ubiquitin, Hsp70, IL-6, C-reactive protein, TWEAK, IL-10, eNOS, endothelin-1, MMP-2, TIMP-2, connexin 43 and superoxide dismutase (SOD) were analysed by immunoblotting. Results: Significant higher levels of eNOS (p=0.011), Cx43 (p=0.020), TIMP-2 (p=0.038) and SOD-3 (p=0.001), with a fold increase of 1.2, 2.1, 1.3 and 1.2 respectively, were found in the EH group. The overall quality of life (60.1 ± 4.3 vs. 53.2 ± 5.9, p=0.009), as well as mental health (59.4 ± 7.9 vs. 50.7 ± 7.2, p=0.024) was significantly higher in the EH group. A strong inverse correlation was observed between the quality of life (total score of the SF-36) and the levels of IL-6 (r=-0.510, p=0.026). The levels of IL-6 are significantly higher in the H group (p=0.014), with a 1.5-fold increase in comparison with the EH group, and the analysis by FTIR showed high correlation between the levels of IL-6 and the 1500-900 cm-1 region of the infrared spectra, in which high levels of IL-6 are characterized by peaks related with collagen and low levels are related to carotenes. Multivariate analysis showed a good discrimination between the two groups in the 1500-900 cm-1 region (fingerprint region) and 1700-1600 cm-1 region (Amide I band). Furthermore, the H group showed peaks that are related with anti-parallel β-sheets, while EH group showed peaks related to parallel β-sheets and α-helices. Conclusion: Regular exercise participation reduced the circulating levels of the inflammatory biomarkers, increased the antioxidant defences and the levels of the vasodilator biomarkers and improved proteostasis and quality of life in individuals with hypertension. |
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Proteostasis, inflamatory and vasoactive biomarkers in patients with hypertension: effect of exercise trainingHypertensionPhysical exerciseBlood pressureBiochemical markersInflammationOxidative stressProteostasisBackground: Physical exercise is a consensual and well-established strategy to control blood pressure. Nonetheless, its effects on protein homeostasis in individuals with hypertension are not clearly defined. Aims: This study aimed to evaluate the effects of regular exercise on inflammatory, oxidative stress and vasoactive markers in circulation, as well as on protein homeostasis and quality of life in individuals with hypertension. Furthermore, we intended to correlate quality of life with the levels of the above-mentioned biomarkers. Methods: A total of 20 individuals with hypertension were recruited and divided into 2 groups of 10: a group who regularly participated in physical exercise (”EH”, age: 68.3 ± 4.2 years), and an age-matched group without regular exercise practice (“H”, age: 67.7 ± 5.1 years). Proteostasis and vasoactive markers were assessed in plasma using immunoblotting techniques (western blot or slot-blot) and Fourier-transform infrared spectroscopy (FTIR). The levels of ubiquitin, Hsp70, IL-6, C-reactive protein, TWEAK, IL-10, eNOS, endothelin-1, MMP-2, TIMP-2, connexin 43 and superoxide dismutase (SOD) were analysed by immunoblotting. Results: Significant higher levels of eNOS (p=0.011), Cx43 (p=0.020), TIMP-2 (p=0.038) and SOD-3 (p=0.001), with a fold increase of 1.2, 2.1, 1.3 and 1.2 respectively, were found in the EH group. The overall quality of life (60.1 ± 4.3 vs. 53.2 ± 5.9, p=0.009), as well as mental health (59.4 ± 7.9 vs. 50.7 ± 7.2, p=0.024) was significantly higher in the EH group. A strong inverse correlation was observed between the quality of life (total score of the SF-36) and the levels of IL-6 (r=-0.510, p=0.026). The levels of IL-6 are significantly higher in the H group (p=0.014), with a 1.5-fold increase in comparison with the EH group, and the analysis by FTIR showed high correlation between the levels of IL-6 and the 1500-900 cm-1 region of the infrared spectra, in which high levels of IL-6 are characterized by peaks related with collagen and low levels are related to carotenes. Multivariate analysis showed a good discrimination between the two groups in the 1500-900 cm-1 region (fingerprint region) and 1700-1600 cm-1 region (Amide I band). Furthermore, the H group showed peaks that are related with anti-parallel β-sheets, while EH group showed peaks related to parallel β-sheets and α-helices. Conclusion: Regular exercise participation reduced the circulating levels of the inflammatory biomarkers, increased the antioxidant defences and the levels of the vasodilator biomarkers and improved proteostasis and quality of life in individuals with hypertension.Introdução: A prática regular de exercício físico é vista como uma estratégia terapêutica consensual no controlo da pressão arterial. No entanto, o seu efeito na homeostasia proteica em indivíduos com hipertensão ainda não está muito bem definido. Objetivo: Com este estudo pretendemos avaliar os efeitos da prática regular de exercício físico em biomarcadores vasoativos, inflamatórios e de stresse oxidativo presentes no plasma, assim como a homeostasia proteica e a qualidade de vida de adultos com hipertensão. Pretendemos também avaliar a correlação entre a qualidade de vida e os níveis dos marcadores acima mencionados. Métodos: Foram recrutados 20 indivíduos hipertensos, divididos em 2 grupos de 10: um grupo com indivíduos que praticavam regularmente exercício físico (grupo ”EH”, idade: 68,3 ± 4,2 anos), e um grupo com caraterísticas idênticas, mas sem prática regular de exercício (grupo “H”, idade: 67,7 ± 5,0 anos). A homeostasia proteica e os marcadores vasoativos foram avaliados através de técnicas de immunoblotting (western blot e slot blot) e da análise de Fourier-transform infrared spectroscopy (FTIR). Os níveis de ubiquitina, Hsp70, IL-6, proteína C-reativa, TWEAK, IL-10, eNOS, endotelina-1, MMP-2, TIMP-2, conexina 43 e SOD foram analisadas por immunoblotting. Resultados: O grupo EH apresentou níveis significativamente maiores de eNOS (p=0.011), Cx43 (p=0.020), TIMP-2 (p=0.038) e SOD-3 (p=0.001). A saúde mental (59.4 ± 7.9 vs. 50.7 ± 7.2, p=0.024) e a qualidade de vida geral (60.1 ± 4.3 vs. 53.2 ± 5.9, p=0.009) foram significativamente maiores no grupo EH. Os níveis de IL-6 foram significativamente maiores no grupo H (p=0.014), com um aumento de 1.5 vezes em relação ao grupo EH. Observou-se uma forte correlação inversa entre a qualidade de vida (pontuação total do SF-36) e os níveis de IL-6 (r=-0.510, p=0.026). A análise por FTIR permitiu correlacionar os níveis de IL-6 com a região 1500-900 cm-1 do espetro de infravermelhos, na qual elevados níveis de IL-6 são caraterizados pelas bandas relacionados com a acumulação de colagénio e níveis baixos estão relacionados com os níveis de carotenos. A análise multivariável permitiu diferenciar os dois grupos na região fingerprint (1500-900 cm-1) e na região da amida I (1700-1600 cm-1). O grupo H é caraterizado por picos relacionados com folhas-β anti-paralelas e o grupo EH é caraterizado por picos relacionados com folhas-β e α-hélices. Conclusão: A prática regular de exercício reduziu os níveis de biomarcadores inflamatórios em circulação, aumentou as defesas antioxidantes e os biomarcadores vasodilatadores, melhorando ainda a homeostasia proteica e a qualidade de vida de indivíduos com hipertensão.2020-12-14T00:00:00Z2018-12-07T00:00:00Z2018-12-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/25071TID:202235335engTeixeira, Manuel António Cardosoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:48:55Zoai:ria.ua.pt:10773/25071Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:58:31.213429Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Proteostasis, inflamatory and vasoactive biomarkers in patients with hypertension: effect of exercise training |
title |
Proteostasis, inflamatory and vasoactive biomarkers in patients with hypertension: effect of exercise training |
spellingShingle |
Proteostasis, inflamatory and vasoactive biomarkers in patients with hypertension: effect of exercise training Teixeira, Manuel António Cardoso Hypertension Physical exercise Blood pressure Biochemical markers Inflammation Oxidative stress Proteostasis |
title_short |
Proteostasis, inflamatory and vasoactive biomarkers in patients with hypertension: effect of exercise training |
title_full |
Proteostasis, inflamatory and vasoactive biomarkers in patients with hypertension: effect of exercise training |
title_fullStr |
Proteostasis, inflamatory and vasoactive biomarkers in patients with hypertension: effect of exercise training |
title_full_unstemmed |
Proteostasis, inflamatory and vasoactive biomarkers in patients with hypertension: effect of exercise training |
title_sort |
Proteostasis, inflamatory and vasoactive biomarkers in patients with hypertension: effect of exercise training |
author |
Teixeira, Manuel António Cardoso |
author_facet |
Teixeira, Manuel António Cardoso |
author_role |
author |
dc.contributor.author.fl_str_mv |
Teixeira, Manuel António Cardoso |
dc.subject.por.fl_str_mv |
Hypertension Physical exercise Blood pressure Biochemical markers Inflammation Oxidative stress Proteostasis |
topic |
Hypertension Physical exercise Blood pressure Biochemical markers Inflammation Oxidative stress Proteostasis |
description |
Background: Physical exercise is a consensual and well-established strategy to control blood pressure. Nonetheless, its effects on protein homeostasis in individuals with hypertension are not clearly defined. Aims: This study aimed to evaluate the effects of regular exercise on inflammatory, oxidative stress and vasoactive markers in circulation, as well as on protein homeostasis and quality of life in individuals with hypertension. Furthermore, we intended to correlate quality of life with the levels of the above-mentioned biomarkers. Methods: A total of 20 individuals with hypertension were recruited and divided into 2 groups of 10: a group who regularly participated in physical exercise (”EH”, age: 68.3 ± 4.2 years), and an age-matched group without regular exercise practice (“H”, age: 67.7 ± 5.1 years). Proteostasis and vasoactive markers were assessed in plasma using immunoblotting techniques (western blot or slot-blot) and Fourier-transform infrared spectroscopy (FTIR). The levels of ubiquitin, Hsp70, IL-6, C-reactive protein, TWEAK, IL-10, eNOS, endothelin-1, MMP-2, TIMP-2, connexin 43 and superoxide dismutase (SOD) were analysed by immunoblotting. Results: Significant higher levels of eNOS (p=0.011), Cx43 (p=0.020), TIMP-2 (p=0.038) and SOD-3 (p=0.001), with a fold increase of 1.2, 2.1, 1.3 and 1.2 respectively, were found in the EH group. The overall quality of life (60.1 ± 4.3 vs. 53.2 ± 5.9, p=0.009), as well as mental health (59.4 ± 7.9 vs. 50.7 ± 7.2, p=0.024) was significantly higher in the EH group. A strong inverse correlation was observed between the quality of life (total score of the SF-36) and the levels of IL-6 (r=-0.510, p=0.026). The levels of IL-6 are significantly higher in the H group (p=0.014), with a 1.5-fold increase in comparison with the EH group, and the analysis by FTIR showed high correlation between the levels of IL-6 and the 1500-900 cm-1 region of the infrared spectra, in which high levels of IL-6 are characterized by peaks related with collagen and low levels are related to carotenes. Multivariate analysis showed a good discrimination between the two groups in the 1500-900 cm-1 region (fingerprint region) and 1700-1600 cm-1 region (Amide I band). Furthermore, the H group showed peaks that are related with anti-parallel β-sheets, while EH group showed peaks related to parallel β-sheets and α-helices. Conclusion: Regular exercise participation reduced the circulating levels of the inflammatory biomarkers, increased the antioxidant defences and the levels of the vasodilator biomarkers and improved proteostasis and quality of life in individuals with hypertension. |
publishDate |
2018 |
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2018-12-07T00:00:00Z 2018-12-07 2020-12-14T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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http://hdl.handle.net/10773/25071 TID:202235335 |
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eng |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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