Total and functional parasite specific IgE responses in Plasmodium falciparum-infected patients exhibiting different clinical status
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/215 http://dx.doi.org/10.1186/1475-2875-6-1 |
Resumo: | Blood samples were collected from controls and P. falciparum-infected patients before treatment on the day of hospitalization (day 0) in India and, in addition, on days 7 and 30 after treatment in Gabon. Total IgE levels were determined by ELISA and functional P. falciparum-specific IgE were estimated using a mast cell line RBL-2H3 transfected with a human Fcε RI α-chain that triggers degranulation upon human IgE cross-linking. Mann Whitney and Kruskall Wallis tests were used to compare groups and the Spearman test was used for correlations. Total IgE levels were confirmed to increase upon infection and differ with level of transmission and age but were not directly related to the disease phenotype. All studied groups exhibited functional parasite-specific IgEs able to induce mast cell degranulation in vitro in the presence of P. falciparum antigens. Plasma IgE levels correlated with those of IL-10 in uncomplicated malaria patients from Gabon. In Indian patients, plasma IFN-γ , TNF and IL-10 levels were significantly correlated with IgE concentrations in all groups. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Total and functional parasite specific IgE responses in Plasmodium falciparum-infected patients exhibiting different clinical statusMalaria, Cerebral/immunologyMalaria, Cerebral/parasitologyMalaria, Cerebral/physiopathologyMalaria, Falciparum/immunologyMalaria, Falciparum/parasitologyMalaria, Falciparum/physiopathologyPlasmodium falciparum/immunologyAntibody SpecificityBlood samples were collected from controls and P. falciparum-infected patients before treatment on the day of hospitalization (day 0) in India and, in addition, on days 7 and 30 after treatment in Gabon. Total IgE levels were determined by ELISA and functional P. falciparum-specific IgE were estimated using a mast cell line RBL-2H3 transfected with a human Fcε RI α-chain that triggers degranulation upon human IgE cross-linking. Mann Whitney and Kruskall Wallis tests were used to compare groups and the Spearman test was used for correlations. Total IgE levels were confirmed to increase upon infection and differ with level of transmission and age but were not directly related to the disease phenotype. All studied groups exhibited functional parasite-specific IgEs able to induce mast cell degranulation in vitro in the presence of P. falciparum antigens. Plasma IgE levels correlated with those of IL-10 in uncomplicated malaria patients from Gabon. In Indian patients, plasma IFN-γ , TNF and IL-10 levels were significantly correlated with IgE concentrations in all groups.Biomed CentralARCADuarte, J.Deshpande, P.Guiyedi, V.Mecheri, S.Fesel, C.Cazenave, P.A.Mishra, G.C.Kombila, M.Pied, S.2011-05-19T09:00:41Z2007-012007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/215http://dx.doi.org/10.1186/1475-2875-6-1eng1475-2875info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-21T14:21:27Zoai:arca.igc.gulbenkian.pt:10400.7/215Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-21T14:21:27Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Total and functional parasite specific IgE responses in Plasmodium falciparum-infected patients exhibiting different clinical status |
title |
Total and functional parasite specific IgE responses in Plasmodium falciparum-infected patients exhibiting different clinical status |
spellingShingle |
Total and functional parasite specific IgE responses in Plasmodium falciparum-infected patients exhibiting different clinical status Duarte, J. Malaria, Cerebral/immunology Malaria, Cerebral/parasitology Malaria, Cerebral/physiopathology Malaria, Falciparum/immunology Malaria, Falciparum/parasitology Malaria, Falciparum/physiopathology Plasmodium falciparum/immunology Antibody Specificity |
title_short |
Total and functional parasite specific IgE responses in Plasmodium falciparum-infected patients exhibiting different clinical status |
title_full |
Total and functional parasite specific IgE responses in Plasmodium falciparum-infected patients exhibiting different clinical status |
title_fullStr |
Total and functional parasite specific IgE responses in Plasmodium falciparum-infected patients exhibiting different clinical status |
title_full_unstemmed |
Total and functional parasite specific IgE responses in Plasmodium falciparum-infected patients exhibiting different clinical status |
title_sort |
Total and functional parasite specific IgE responses in Plasmodium falciparum-infected patients exhibiting different clinical status |
author |
Duarte, J. |
author_facet |
Duarte, J. Deshpande, P. Guiyedi, V. Mecheri, S. Fesel, C. Cazenave, P.A. Mishra, G.C. Kombila, M. Pied, S. |
author_role |
author |
author2 |
Deshpande, P. Guiyedi, V. Mecheri, S. Fesel, C. Cazenave, P.A. Mishra, G.C. Kombila, M. Pied, S. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Duarte, J. Deshpande, P. Guiyedi, V. Mecheri, S. Fesel, C. Cazenave, P.A. Mishra, G.C. Kombila, M. Pied, S. |
dc.subject.por.fl_str_mv |
Malaria, Cerebral/immunology Malaria, Cerebral/parasitology Malaria, Cerebral/physiopathology Malaria, Falciparum/immunology Malaria, Falciparum/parasitology Malaria, Falciparum/physiopathology Plasmodium falciparum/immunology Antibody Specificity |
topic |
Malaria, Cerebral/immunology Malaria, Cerebral/parasitology Malaria, Cerebral/physiopathology Malaria, Falciparum/immunology Malaria, Falciparum/parasitology Malaria, Falciparum/physiopathology Plasmodium falciparum/immunology Antibody Specificity |
description |
Blood samples were collected from controls and P. falciparum-infected patients before treatment on the day of hospitalization (day 0) in India and, in addition, on days 7 and 30 after treatment in Gabon. Total IgE levels were determined by ELISA and functional P. falciparum-specific IgE were estimated using a mast cell line RBL-2H3 transfected with a human Fcε RI α-chain that triggers degranulation upon human IgE cross-linking. Mann Whitney and Kruskall Wallis tests were used to compare groups and the Spearman test was used for correlations. Total IgE levels were confirmed to increase upon infection and differ with level of transmission and age but were not directly related to the disease phenotype. All studied groups exhibited functional parasite-specific IgEs able to induce mast cell degranulation in vitro in the presence of P. falciparum antigens. Plasma IgE levels correlated with those of IL-10 in uncomplicated malaria patients from Gabon. In Indian patients, plasma IFN-γ , TNF and IL-10 levels were significantly correlated with IgE concentrations in all groups. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-01 2007-01-01T00:00:00Z 2011-05-19T09:00:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/215 http://dx.doi.org/10.1186/1475-2875-6-1 |
url |
http://hdl.handle.net/10400.7/215 http://dx.doi.org/10.1186/1475-2875-6-1 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1475-2875 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central |
publisher.none.fl_str_mv |
Biomed Central |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
_version_ |
1817549561651003392 |