Development of a DoE with a new electrospinning system for cartilage tissue engineering
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/32835 |
Resumo: | Electrospinning is currently one of the most used techniques to produce fibrous synthetic tissues such as cartilage and bone. To replicate cartilage tissue engineering functionality, one of the most important characteristics is the alignment of the resulting fibre meshes in a three-dimensional (3D) fashion. Here, a newly developed electrospinning collector system is tested in order to understand how the process parameters affected the obtained fibre meshes topography. For that, a polymer consisting of PCL/Gelatin was electrospun using the electrostatic potential to create a fibre mesh. A Design of the Experiments (DoE) approach was implemented, to determine whether the variation of the main process parameters led to significant effects on the mesh dimensional characteristics. The process parameters analyzed were the velocity of the collecting bands, the linear velocity of the fibre deposition table and the flow rate. The analyzed mesh characteristics were the fibre diameter, the distance between the fibres and pore size. The effect of each of the three factors was statistically analyzed using ANOVA, as well as the interaction between them. Complementary an ANOVA linear regression approach was developed to predict the distance between the fibres. This statistical regression was then compared with a predictive theoretical model and with the experimental results. The results obtained indicate the presence of interactions between the three process parameters analyzed. The three process parameters showed statistical significance in the distance between the fibres, however, the velocity of the deposition table was the process parameter that presented the highest effect. |
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Development of a DoE with a new electrospinning system for cartilage tissue engineeringElectrospinningNanofiber alignmentBiomechanicsTissue engineeringDesign of experimentsBiofabricationElectrospinning is currently one of the most used techniques to produce fibrous synthetic tissues such as cartilage and bone. To replicate cartilage tissue engineering functionality, one of the most important characteristics is the alignment of the resulting fibre meshes in a three-dimensional (3D) fashion. Here, a newly developed electrospinning collector system is tested in order to understand how the process parameters affected the obtained fibre meshes topography. For that, a polymer consisting of PCL/Gelatin was electrospun using the electrostatic potential to create a fibre mesh. A Design of the Experiments (DoE) approach was implemented, to determine whether the variation of the main process parameters led to significant effects on the mesh dimensional characteristics. The process parameters analyzed were the velocity of the collecting bands, the linear velocity of the fibre deposition table and the flow rate. The analyzed mesh characteristics were the fibre diameter, the distance between the fibres and pore size. The effect of each of the three factors was statistically analyzed using ANOVA, as well as the interaction between them. Complementary an ANOVA linear regression approach was developed to predict the distance between the fibres. This statistical regression was then compared with a predictive theoretical model and with the experimental results. The results obtained indicate the presence of interactions between the three process parameters analyzed. The three process parameters showed statistical significance in the distance between the fibres, however, the velocity of the deposition table was the process parameter that presented the highest effect.Publicações ISEP2022-01-07T16:24:52Z2021-11-18T00:00:00Z2021-11-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/32835eng2184-897110.5281/zenodo.5710429Silva, E.Semitela, A.Marques, P. A. A. P.Completo, A.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:03:07Zoai:ria.ua.pt:10773/32835Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:04:21.007547Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development of a DoE with a new electrospinning system for cartilage tissue engineering |
title |
Development of a DoE with a new electrospinning system for cartilage tissue engineering |
spellingShingle |
Development of a DoE with a new electrospinning system for cartilage tissue engineering Silva, E. Electrospinning Nanofiber alignment Biomechanics Tissue engineering Design of experiments Biofabrication |
title_short |
Development of a DoE with a new electrospinning system for cartilage tissue engineering |
title_full |
Development of a DoE with a new electrospinning system for cartilage tissue engineering |
title_fullStr |
Development of a DoE with a new electrospinning system for cartilage tissue engineering |
title_full_unstemmed |
Development of a DoE with a new electrospinning system for cartilage tissue engineering |
title_sort |
Development of a DoE with a new electrospinning system for cartilage tissue engineering |
author |
Silva, E. |
author_facet |
Silva, E. Semitela, A. Marques, P. A. A. P. Completo, A. |
author_role |
author |
author2 |
Semitela, A. Marques, P. A. A. P. Completo, A. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Silva, E. Semitela, A. Marques, P. A. A. P. Completo, A. |
dc.subject.por.fl_str_mv |
Electrospinning Nanofiber alignment Biomechanics Tissue engineering Design of experiments Biofabrication |
topic |
Electrospinning Nanofiber alignment Biomechanics Tissue engineering Design of experiments Biofabrication |
description |
Electrospinning is currently one of the most used techniques to produce fibrous synthetic tissues such as cartilage and bone. To replicate cartilage tissue engineering functionality, one of the most important characteristics is the alignment of the resulting fibre meshes in a three-dimensional (3D) fashion. Here, a newly developed electrospinning collector system is tested in order to understand how the process parameters affected the obtained fibre meshes topography. For that, a polymer consisting of PCL/Gelatin was electrospun using the electrostatic potential to create a fibre mesh. A Design of the Experiments (DoE) approach was implemented, to determine whether the variation of the main process parameters led to significant effects on the mesh dimensional characteristics. The process parameters analyzed were the velocity of the collecting bands, the linear velocity of the fibre deposition table and the flow rate. The analyzed mesh characteristics were the fibre diameter, the distance between the fibres and pore size. The effect of each of the three factors was statistically analyzed using ANOVA, as well as the interaction between them. Complementary an ANOVA linear regression approach was developed to predict the distance between the fibres. This statistical regression was then compared with a predictive theoretical model and with the experimental results. The results obtained indicate the presence of interactions between the three process parameters analyzed. The three process parameters showed statistical significance in the distance between the fibres, however, the velocity of the deposition table was the process parameter that presented the highest effect. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-18T00:00:00Z 2021-11-18 2022-01-07T16:24:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/32835 |
url |
http://hdl.handle.net/10773/32835 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2184-8971 10.5281/zenodo.5710429 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Publicações ISEP |
publisher.none.fl_str_mv |
Publicações ISEP |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137698901917696 |