Development of a DoE with a new electrospinning system for cartilage tissue engineering

Detalhes bibliográficos
Autor(a) principal: Silva, E.
Data de Publicação: 2021
Outros Autores: Semitela, A., Marques, P. A. A. P., Completo, A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/32835
Resumo: Electrospinning is currently one of the most used techniques to produce fibrous synthetic tissues such as cartilage and bone. To replicate cartilage tissue engineering functionality, one of the most important characteristics is the alignment of the resulting fibre meshes in a three-dimensional (3D) fashion. Here, a newly developed electrospinning collector system is tested in order to understand how the process parameters affected the obtained fibre meshes topography. For that, a polymer consisting of PCL/Gelatin was electrospun using the electrostatic potential to create a fibre mesh. A Design of the Experiments (DoE) approach was implemented, to determine whether the variation of the main process parameters led to significant effects on the mesh dimensional characteristics. The process parameters analyzed were the velocity of the collecting bands, the linear velocity of the fibre deposition table and the flow rate. The analyzed mesh characteristics were the fibre diameter, the distance between the fibres and pore size. The effect of each of the three factors was statistically analyzed using ANOVA, as well as the interaction between them. Complementary an ANOVA linear regression approach was developed to predict the distance between the fibres. This statistical regression was then compared with a predictive theoretical model and with the experimental results. The results obtained indicate the presence of interactions between the three process parameters analyzed. The three process parameters showed statistical significance in the distance between the fibres, however, the velocity of the deposition table was the process parameter that presented the highest effect.
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spelling Development of a DoE with a new electrospinning system for cartilage tissue engineeringElectrospinningNanofiber alignmentBiomechanicsTissue engineeringDesign of experimentsBiofabricationElectrospinning is currently one of the most used techniques to produce fibrous synthetic tissues such as cartilage and bone. To replicate cartilage tissue engineering functionality, one of the most important characteristics is the alignment of the resulting fibre meshes in a three-dimensional (3D) fashion. Here, a newly developed electrospinning collector system is tested in order to understand how the process parameters affected the obtained fibre meshes topography. For that, a polymer consisting of PCL/Gelatin was electrospun using the electrostatic potential to create a fibre mesh. A Design of the Experiments (DoE) approach was implemented, to determine whether the variation of the main process parameters led to significant effects on the mesh dimensional characteristics. The process parameters analyzed were the velocity of the collecting bands, the linear velocity of the fibre deposition table and the flow rate. The analyzed mesh characteristics were the fibre diameter, the distance between the fibres and pore size. The effect of each of the three factors was statistically analyzed using ANOVA, as well as the interaction between them. Complementary an ANOVA linear regression approach was developed to predict the distance between the fibres. This statistical regression was then compared with a predictive theoretical model and with the experimental results. The results obtained indicate the presence of interactions between the three process parameters analyzed. The three process parameters showed statistical significance in the distance between the fibres, however, the velocity of the deposition table was the process parameter that presented the highest effect.Publicações ISEP2022-01-07T16:24:52Z2021-11-18T00:00:00Z2021-11-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/32835eng2184-897110.5281/zenodo.5710429Silva, E.Semitela, A.Marques, P. A. A. P.Completo, A.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:03:07Zoai:ria.ua.pt:10773/32835Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:04:21.007547Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development of a DoE with a new electrospinning system for cartilage tissue engineering
title Development of a DoE with a new electrospinning system for cartilage tissue engineering
spellingShingle Development of a DoE with a new electrospinning system for cartilage tissue engineering
Silva, E.
Electrospinning
Nanofiber alignment
Biomechanics
Tissue engineering
Design of experiments
Biofabrication
title_short Development of a DoE with a new electrospinning system for cartilage tissue engineering
title_full Development of a DoE with a new electrospinning system for cartilage tissue engineering
title_fullStr Development of a DoE with a new electrospinning system for cartilage tissue engineering
title_full_unstemmed Development of a DoE with a new electrospinning system for cartilage tissue engineering
title_sort Development of a DoE with a new electrospinning system for cartilage tissue engineering
author Silva, E.
author_facet Silva, E.
Semitela, A.
Marques, P. A. A. P.
Completo, A.
author_role author
author2 Semitela, A.
Marques, P. A. A. P.
Completo, A.
author2_role author
author
author
dc.contributor.author.fl_str_mv Silva, E.
Semitela, A.
Marques, P. A. A. P.
Completo, A.
dc.subject.por.fl_str_mv Electrospinning
Nanofiber alignment
Biomechanics
Tissue engineering
Design of experiments
Biofabrication
topic Electrospinning
Nanofiber alignment
Biomechanics
Tissue engineering
Design of experiments
Biofabrication
description Electrospinning is currently one of the most used techniques to produce fibrous synthetic tissues such as cartilage and bone. To replicate cartilage tissue engineering functionality, one of the most important characteristics is the alignment of the resulting fibre meshes in a three-dimensional (3D) fashion. Here, a newly developed electrospinning collector system is tested in order to understand how the process parameters affected the obtained fibre meshes topography. For that, a polymer consisting of PCL/Gelatin was electrospun using the electrostatic potential to create a fibre mesh. A Design of the Experiments (DoE) approach was implemented, to determine whether the variation of the main process parameters led to significant effects on the mesh dimensional characteristics. The process parameters analyzed were the velocity of the collecting bands, the linear velocity of the fibre deposition table and the flow rate. The analyzed mesh characteristics were the fibre diameter, the distance between the fibres and pore size. The effect of each of the three factors was statistically analyzed using ANOVA, as well as the interaction between them. Complementary an ANOVA linear regression approach was developed to predict the distance between the fibres. This statistical regression was then compared with a predictive theoretical model and with the experimental results. The results obtained indicate the presence of interactions between the three process parameters analyzed. The three process parameters showed statistical significance in the distance between the fibres, however, the velocity of the deposition table was the process parameter that presented the highest effect.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-18T00:00:00Z
2021-11-18
2022-01-07T16:24:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/32835
url http://hdl.handle.net/10773/32835
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2184-8971
10.5281/zenodo.5710429
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dc.publisher.none.fl_str_mv Publicações ISEP
publisher.none.fl_str_mv Publicações ISEP
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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