Development of an experiment plan with 3D electrospinning equipment for cartilage tissue engineering
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/34367 |
Resumo: | One of the major challenges associated with tissue engineering cartilage is the difficulty to recreate the anisotropic organization of the fibers, in order to simulate the biomechanical properties of the native tissue. The main objective of this dissertation was the development and application of a plan of experiments with 3D electrospinning equipment for tissue engineering cartilage. This plan of experiments seeks to understand how the combination of different parameters associated with the used 3D electrospinning equipment, influences the characteristics and properties of the resulting scaffolds. In this dissertation, in an initial phase, a description of cartilage is presented in terms of its structure, function, and biomechanical properties. It was also carried out an evaluation of the biofabrication processes in tissue engineering, focusing on cartilage engineering and in particular in electrospinning processes with controlled fiber alignment. In another stage, the plan of experiments was established, with identification of the input variables and respective levels that were going to be controlled in the equipment, as well as the identification of the parameters that were analysed in the produced scaffolds, being that the input variables and the respective levels varied in function of the architecture of the scaffold. Three scaffolds architectures were selected from the development in previous works. The input variables of the electrospinning process that were associated with two levels were: the electrospun PCL flow rate, the deposition time in static alignment (line), the number of deposition cycles in angular rotation (mesh), the collector disks velocity and the total number of arcade alignment cycles. The analysed parameters in the produced scaffolds for different combination of input variables/levels were: the thickness, the weight, the three-dimensional organization of the scaffold, the fiber diameter, porosity, and the elastic modulus at compression for 10% deformation. An analysis of variance (ANOVA) was applied to determine the effects of the controlled input variables in the electrospinning process on the analysed parameters in the resulting scaffolds. In the results obtained for the three architectures of scaffolds, the flow rate variable presented statistical significance (p<0.05). The deposition time in static alignment (line) presented significance for some output parameters and in fuction of the architecture. For the number of deposition cycles in angular rotation (mesh) variable it showed significant effects for the generality of the output parameters in the second scaffold architecture. The number of total cycles in the third architecture had a statistical significance in the thickness and weight of the scaffold parameters, while the velocity of the collector disks presented significance for the thickness, fiber diameter and elastic modulus at compression parameters. |
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Development of an experiment plan with 3D electrospinning equipment for cartilage tissue engineeringCartilageTissue engineering3D electrospinningAnisotropyBiomechanicBiofabricationDesign of experiementsOne of the major challenges associated with tissue engineering cartilage is the difficulty to recreate the anisotropic organization of the fibers, in order to simulate the biomechanical properties of the native tissue. The main objective of this dissertation was the development and application of a plan of experiments with 3D electrospinning equipment for tissue engineering cartilage. This plan of experiments seeks to understand how the combination of different parameters associated with the used 3D electrospinning equipment, influences the characteristics and properties of the resulting scaffolds. In this dissertation, in an initial phase, a description of cartilage is presented in terms of its structure, function, and biomechanical properties. It was also carried out an evaluation of the biofabrication processes in tissue engineering, focusing on cartilage engineering and in particular in electrospinning processes with controlled fiber alignment. In another stage, the plan of experiments was established, with identification of the input variables and respective levels that were going to be controlled in the equipment, as well as the identification of the parameters that were analysed in the produced scaffolds, being that the input variables and the respective levels varied in function of the architecture of the scaffold. Three scaffolds architectures were selected from the development in previous works. The input variables of the electrospinning process that were associated with two levels were: the electrospun PCL flow rate, the deposition time in static alignment (line), the number of deposition cycles in angular rotation (mesh), the collector disks velocity and the total number of arcade alignment cycles. The analysed parameters in the produced scaffolds for different combination of input variables/levels were: the thickness, the weight, the three-dimensional organization of the scaffold, the fiber diameter, porosity, and the elastic modulus at compression for 10% deformation. An analysis of variance (ANOVA) was applied to determine the effects of the controlled input variables in the electrospinning process on the analysed parameters in the resulting scaffolds. In the results obtained for the three architectures of scaffolds, the flow rate variable presented statistical significance (p<0.05). The deposition time in static alignment (line) presented significance for some output parameters and in fuction of the architecture. For the number of deposition cycles in angular rotation (mesh) variable it showed significant effects for the generality of the output parameters in the second scaffold architecture. The number of total cycles in the third architecture had a statistical significance in the thickness and weight of the scaffold parameters, while the velocity of the collector disks presented significance for the thickness, fiber diameter and elastic modulus at compression parameters.Um dos maiores desafios associado à engenharia dos tecidos de cartilagem e a dificuldade de recriar a organização anisotrópica das suas fibras, de maneira a se aproximar do comportamento e propriedades biomecânicas do tecido nativo. Este trabalho teve como objetivo o desenvolvimento e implementação de um plano de experiências com um equipamento de eletrofiação 3D para a engenharia de tecidos de cartilagem. Este plano de experiências procura entender como a combinação de diferentes parâmetros associados ao processo de eletrofiação 3D do equipamento utilizado, influenciam as características e propriedades das estruturas fibrosas tridimensionais “scaffolds” produzidas. Neste trabalho numa fase inicial é realizada uma descrição da cartilagem em termos de sua estrutura, função e propriedades biomecânicas. Foi também realizado uma avaliação dos processos de biofabricação na engenharia de tecidos com especial enfoque na engenharia de cartilagem e em particular nos processos de eletrofiação com alinhamento controlado das fibras. Numa outra etapa procedeu-se ao estabelecimento do plano de experiências com a identificação dos variáveis e respetivos níveis a controlar no equipamento assim como a identificação dos parâmetros a avaliar nas estruturas tridimensionais produzidas, sendo que as variáveis de entrada e respetivos níveis variaram em função do tipo de arquitetura da estrutura tridimensional produzida. Foram selecionadas três arquiteturas de “scaffolds” já desenvolvidas no âmbito de trabalhos anteriores. As variáveis de entrada do processo de eletrofiação que foram associadas a dois níveis foram: o fluxo do polímero PCL eletrofiado, o tempo de deposição em alinhamento estático (linha), o número de ciclos de deposição em rotação angular (malha), a velocidade de rotação dos rolos coletores e o número total de ciclos de alinhamento em arcada. Os parâmetros analisados nos “scaffolds” fabricados para as diferentes combinações das variáveis/níveis de entrada foram: a espessura, o peso, a organização tridimensional do “scaffold”, o diâmetro das fibras, a porosidade e o modulo de elasticidade à compressão a 10% de deformação. Foi aplicada uma análise de variância (ANOVA) para determinar os efeitos das variáveis controladas no processo de eletrofialão nos parâmetros analisados nas estruturas 3D resultantes. Nos resultados obtidos para as três arquiteturas de “scaffolds”, a variável do processo fluxo de polímero apresentou significado estatístico (p<0.05) para a generalidade das variáveis de saída analisadas. Já o tempo de deposição em alinhamento estático (linha) apresentou alguma significância para apenas algumas das variáveis de saída e em função do tipo de arquitetura do “scaffold”. Relativamente à variável número de ciclos de deposição em rotação angular (malha) teve efeitos significativos para a generalidade das variáveis de saída na segunda arquitetura de “scaffold”. Já o número de ciclos totais na terceira arquitetura analisada teve um significado estatístico nos parâmetros espessura e peso do “scaffold” enquanto a variável velocidade dos rolos coletores apresentou significância nos parâmetros espessura, diâmetro de fibra e modulo de elasticidade à compressão.2022-08-02T09:49:00Z2022-07-12T00:00:00Z2022-07-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/34367engAmador, Rita Costainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:06:26Zoai:ria.ua.pt:10773/34367Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:05:44.265863Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development of an experiment plan with 3D electrospinning equipment for cartilage tissue engineering |
title |
Development of an experiment plan with 3D electrospinning equipment for cartilage tissue engineering |
spellingShingle |
Development of an experiment plan with 3D electrospinning equipment for cartilage tissue engineering Amador, Rita Costa Cartilage Tissue engineering 3D electrospinning Anisotropy Biomechanic Biofabrication Design of experiements |
title_short |
Development of an experiment plan with 3D electrospinning equipment for cartilage tissue engineering |
title_full |
Development of an experiment plan with 3D electrospinning equipment for cartilage tissue engineering |
title_fullStr |
Development of an experiment plan with 3D electrospinning equipment for cartilage tissue engineering |
title_full_unstemmed |
Development of an experiment plan with 3D electrospinning equipment for cartilage tissue engineering |
title_sort |
Development of an experiment plan with 3D electrospinning equipment for cartilage tissue engineering |
author |
Amador, Rita Costa |
author_facet |
Amador, Rita Costa |
author_role |
author |
dc.contributor.author.fl_str_mv |
Amador, Rita Costa |
dc.subject.por.fl_str_mv |
Cartilage Tissue engineering 3D electrospinning Anisotropy Biomechanic Biofabrication Design of experiements |
topic |
Cartilage Tissue engineering 3D electrospinning Anisotropy Biomechanic Biofabrication Design of experiements |
description |
One of the major challenges associated with tissue engineering cartilage is the difficulty to recreate the anisotropic organization of the fibers, in order to simulate the biomechanical properties of the native tissue. The main objective of this dissertation was the development and application of a plan of experiments with 3D electrospinning equipment for tissue engineering cartilage. This plan of experiments seeks to understand how the combination of different parameters associated with the used 3D electrospinning equipment, influences the characteristics and properties of the resulting scaffolds. In this dissertation, in an initial phase, a description of cartilage is presented in terms of its structure, function, and biomechanical properties. It was also carried out an evaluation of the biofabrication processes in tissue engineering, focusing on cartilage engineering and in particular in electrospinning processes with controlled fiber alignment. In another stage, the plan of experiments was established, with identification of the input variables and respective levels that were going to be controlled in the equipment, as well as the identification of the parameters that were analysed in the produced scaffolds, being that the input variables and the respective levels varied in function of the architecture of the scaffold. Three scaffolds architectures were selected from the development in previous works. The input variables of the electrospinning process that were associated with two levels were: the electrospun PCL flow rate, the deposition time in static alignment (line), the number of deposition cycles in angular rotation (mesh), the collector disks velocity and the total number of arcade alignment cycles. The analysed parameters in the produced scaffolds for different combination of input variables/levels were: the thickness, the weight, the three-dimensional organization of the scaffold, the fiber diameter, porosity, and the elastic modulus at compression for 10% deformation. An analysis of variance (ANOVA) was applied to determine the effects of the controlled input variables in the electrospinning process on the analysed parameters in the resulting scaffolds. In the results obtained for the three architectures of scaffolds, the flow rate variable presented statistical significance (p<0.05). The deposition time in static alignment (line) presented significance for some output parameters and in fuction of the architecture. For the number of deposition cycles in angular rotation (mesh) variable it showed significant effects for the generality of the output parameters in the second scaffold architecture. The number of total cycles in the third architecture had a statistical significance in the thickness and weight of the scaffold parameters, while the velocity of the collector disks presented significance for the thickness, fiber diameter and elastic modulus at compression parameters. |
publishDate |
2022 |
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2022-08-02T09:49:00Z 2022-07-12T00:00:00Z 2022-07-12 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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http://hdl.handle.net/10773/34367 |
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http://hdl.handle.net/10773/34367 |
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eng |
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eng |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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