Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells

Detalhes bibliográficos
Autor(a) principal: Amorim, Sara
Data de Publicação: 2020
Outros Autores: Costa, Diana Soares da, Pashkuleva, Iva, Reis, Celso A., Reis, R. L., Pires, R. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/67337
Resumo: The overproduction and deposition of hyaluronic acid (HA) of different sizes in the tumor microenvironment is associated with cancer metastasis. Here, the development of layerâ byâ layer (LbL) constructs containing HA of different molecular weights (i.e., 5.6, 618, and 1450 kDa) that mimic the HAâ rich cancer extracellular matrix is described to study the effect of the HA's size on the behavior of gastric cancer cells (AGS). The results demonstrate that LbL constructs with short HA, i.e., 5.6 kDa, activate the cytoskeleton rearrangement leading to the â hummingbirdâ morphology, promote high cellular motility, and activate signaling pathways with increased expression of pâ ERK1/2 and pâ AKT. In addition, it is demonstrated that this malignant transformation involves an active participation of the HA coreceptor RHAMM in AGS cells.
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spelling Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cellsGastric cancerHummingbird phenotypeHyaluronic acidcancer invasivenesshummingbirdmolecular weightScience & TechnologyThe overproduction and deposition of hyaluronic acid (HA) of different sizes in the tumor microenvironment is associated with cancer metastasis. Here, the development of layerâ byâ layer (LbL) constructs containing HA of different molecular weights (i.e., 5.6, 618, and 1450 kDa) that mimic the HAâ rich cancer extracellular matrix is described to study the effect of the HA's size on the behavior of gastric cancer cells (AGS). The results demonstrate that LbL constructs with short HA, i.e., 5.6 kDa, activate the cytoskeleton rearrangement leading to the â hummingbirdâ morphology, promote high cellular motility, and activate signaling pathways with increased expression of pâ ERK1/2 and pâ AKT. In addition, it is demonstrated that this malignant transformation involves an active participation of the HA coreceptor RHAMM in AGS cells.The authors acknowledge the financial support from the European Commission’s H2020 Programme, under grant agreements H2020-WIDESPREAD-2014-668983-FORECAST and H2020-MSCA-RISE-2019-872648-MEPHOS. S.A. acknowledge the Portuguese Foundation for Science and Technology (FCT) for the PhD grant (SFRH/BD/112075/2015). The authors also thank Ramon Novoa-Caballal for performing the GPC experiments and data analysis.WileyUniversidade do MinhoAmorim, SaraCosta, Diana Soares daPashkuleva, IvaReis, Celso A.Reis, R. L.Pires, R. A.20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/67337engAmorim S., Soares da Costa D., Pashkuleva I., Reis C. A., Reis R. L., Pires R. A. Hyaluronic Acid of Low Molecular Weight Triggers the Invasive “Hummingbird” Phenotype on Gastric Cancer Cells, Advanced Biosystems, pp. 2000122, doi:10.1002/adbi.202000122, 20202366-747810.1002/adbi.202000122https://onlinelibrary.wiley.com/doi/10.1002/adbi.202000122info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:17:02Zoai:repositorium.sdum.uminho.pt:1822/67337Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:09:35.378591Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells
title Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells
spellingShingle Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells
Amorim, Sara
Gastric cancer
Hummingbird phenotype
Hyaluronic acid
cancer invasiveness
hummingbird
molecular weight
Science & Technology
title_short Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells
title_full Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells
title_fullStr Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells
title_full_unstemmed Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells
title_sort Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells
author Amorim, Sara
author_facet Amorim, Sara
Costa, Diana Soares da
Pashkuleva, Iva
Reis, Celso A.
Reis, R. L.
Pires, R. A.
author_role author
author2 Costa, Diana Soares da
Pashkuleva, Iva
Reis, Celso A.
Reis, R. L.
Pires, R. A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Amorim, Sara
Costa, Diana Soares da
Pashkuleva, Iva
Reis, Celso A.
Reis, R. L.
Pires, R. A.
dc.subject.por.fl_str_mv Gastric cancer
Hummingbird phenotype
Hyaluronic acid
cancer invasiveness
hummingbird
molecular weight
Science & Technology
topic Gastric cancer
Hummingbird phenotype
Hyaluronic acid
cancer invasiveness
hummingbird
molecular weight
Science & Technology
description The overproduction and deposition of hyaluronic acid (HA) of different sizes in the tumor microenvironment is associated with cancer metastasis. Here, the development of layerâ byâ layer (LbL) constructs containing HA of different molecular weights (i.e., 5.6, 618, and 1450 kDa) that mimic the HAâ rich cancer extracellular matrix is described to study the effect of the HA's size on the behavior of gastric cancer cells (AGS). The results demonstrate that LbL constructs with short HA, i.e., 5.6 kDa, activate the cytoskeleton rearrangement leading to the â hummingbirdâ morphology, promote high cellular motility, and activate signaling pathways with increased expression of pâ ERK1/2 and pâ AKT. In addition, it is demonstrated that this malignant transformation involves an active participation of the HA coreceptor RHAMM in AGS cells.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/67337
url http://hdl.handle.net/1822/67337
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Amorim S., Soares da Costa D., Pashkuleva I., Reis C. A., Reis R. L., Pires R. A. Hyaluronic Acid of Low Molecular Weight Triggers the Invasive “Hummingbird” Phenotype on Gastric Cancer Cells, Advanced Biosystems, pp. 2000122, doi:10.1002/adbi.202000122, 2020
2366-7478
10.1002/adbi.202000122
https://onlinelibrary.wiley.com/doi/10.1002/adbi.202000122
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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