Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/67337 |
Resumo: | The overproduction and deposition of hyaluronic acid (HA) of different sizes in the tumor microenvironment is associated with cancer metastasis. Here, the development of layerâ byâ layer (LbL) constructs containing HA of different molecular weights (i.e., 5.6, 618, and 1450Â kDa) that mimic the HAâ rich cancer extracellular matrix is described to study the effect of the HA's size on the behavior of gastric cancer cells (AGS). The results demonstrate that LbL constructs with short HA, i.e., 5.6Â kDa, activate the cytoskeleton rearrangement leading to the â hummingbirdâ morphology, promote high cellular motility, and activate signaling pathways with increased expression of pâ ERK1/2 and pâ AKT. In addition, it is demonstrated that this malignant transformation involves an active participation of the HA coreceptor RHAMM in AGS cells. |
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Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cellsGastric cancerHummingbird phenotypeHyaluronic acidcancer invasivenesshummingbirdmolecular weightScience & TechnologyThe overproduction and deposition of hyaluronic acid (HA) of different sizes in the tumor microenvironment is associated with cancer metastasis. Here, the development of layerâ byâ layer (LbL) constructs containing HA of different molecular weights (i.e., 5.6, 618, and 1450Â kDa) that mimic the HAâ rich cancer extracellular matrix is described to study the effect of the HA's size on the behavior of gastric cancer cells (AGS). The results demonstrate that LbL constructs with short HA, i.e., 5.6Â kDa, activate the cytoskeleton rearrangement leading to the â hummingbirdâ morphology, promote high cellular motility, and activate signaling pathways with increased expression of pâ ERK1/2 and pâ AKT. In addition, it is demonstrated that this malignant transformation involves an active participation of the HA coreceptor RHAMM in AGS cells.The authors acknowledge the financial support from the European Commission’s H2020 Programme, under grant agreements H2020-WIDESPREAD-2014-668983-FORECAST and H2020-MSCA-RISE-2019-872648-MEPHOS. S.A. acknowledge the Portuguese Foundation for Science and Technology (FCT) for the PhD grant (SFRH/BD/112075/2015). The authors also thank Ramon Novoa-Caballal for performing the GPC experiments and data analysis.WileyUniversidade do MinhoAmorim, SaraCosta, Diana Soares daPashkuleva, IvaReis, Celso A.Reis, R. L.Pires, R. A.20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/67337engAmorim S., Soares da Costa D., Pashkuleva I., Reis C. A., Reis R. L., Pires R. A. Hyaluronic Acid of Low Molecular Weight Triggers the Invasive “Hummingbird” Phenotype on Gastric Cancer Cells, Advanced Biosystems, pp. 2000122, doi:10.1002/adbi.202000122, 20202366-747810.1002/adbi.202000122https://onlinelibrary.wiley.com/doi/10.1002/adbi.202000122info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:17:02Zoai:repositorium.sdum.uminho.pt:1822/67337Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:09:35.378591Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells |
title |
Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells |
spellingShingle |
Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells Amorim, Sara Gastric cancer Hummingbird phenotype Hyaluronic acid cancer invasiveness hummingbird molecular weight Science & Technology |
title_short |
Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells |
title_full |
Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells |
title_fullStr |
Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells |
title_full_unstemmed |
Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells |
title_sort |
Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells |
author |
Amorim, Sara |
author_facet |
Amorim, Sara Costa, Diana Soares da Pashkuleva, Iva Reis, Celso A. Reis, R. L. Pires, R. A. |
author_role |
author |
author2 |
Costa, Diana Soares da Pashkuleva, Iva Reis, Celso A. Reis, R. L. Pires, R. A. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Amorim, Sara Costa, Diana Soares da Pashkuleva, Iva Reis, Celso A. Reis, R. L. Pires, R. A. |
dc.subject.por.fl_str_mv |
Gastric cancer Hummingbird phenotype Hyaluronic acid cancer invasiveness hummingbird molecular weight Science & Technology |
topic |
Gastric cancer Hummingbird phenotype Hyaluronic acid cancer invasiveness hummingbird molecular weight Science & Technology |
description |
The overproduction and deposition of hyaluronic acid (HA) of different sizes in the tumor microenvironment is associated with cancer metastasis. Here, the development of layerâ byâ layer (LbL) constructs containing HA of different molecular weights (i.e., 5.6, 618, and 1450Â kDa) that mimic the HAâ rich cancer extracellular matrix is described to study the effect of the HA's size on the behavior of gastric cancer cells (AGS). The results demonstrate that LbL constructs with short HA, i.e., 5.6Â kDa, activate the cytoskeleton rearrangement leading to the â hummingbirdâ morphology, promote high cellular motility, and activate signaling pathways with increased expression of pâ ERK1/2 and pâ AKT. In addition, it is demonstrated that this malignant transformation involves an active participation of the HA coreceptor RHAMM in AGS cells. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/67337 |
url |
http://hdl.handle.net/1822/67337 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Amorim S., Soares da Costa D., Pashkuleva I., Reis C. A., Reis R. L., Pires R. A. Hyaluronic Acid of Low Molecular Weight Triggers the Invasive “Hummingbird” Phenotype on Gastric Cancer Cells, Advanced Biosystems, pp. 2000122, doi:10.1002/adbi.202000122, 2020 2366-7478 10.1002/adbi.202000122 https://onlinelibrary.wiley.com/doi/10.1002/adbi.202000122 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132522213277696 |