Solea senegalensis sperm cryopreservation: new insights on sperm quality
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/11872 |
Resumo: | Cryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough information to identify specific damage caused by cryopreservation process (freezing and thawing). Specific tests, including lipid peroxidation and DNA damage, should be performed. In the present study, motility and lipid peroxidation were performed as specific tests allowing us to discard cryopreservation conditions such as methanol as internal cryoprotectant and bovine serum albumin as external cryoprotectant. In addition, a caspase 3/7 detection by flow cytometry was performed to analyze apoptosis activity in the best selected conditions. Moreover, new highly sensitive tests based on transcript number detection have recently been described in fish sperm cryopreservation. For this reason, a transcript level detection assay was performed on certain oxidative and chaperone genes related to fertilization ability and embryo development (hsp70, hsp90BB, hsp90AA, gpx) to select the best cryopreservation conditions. DMSO+ egg yolk proved to be the best cryoprotectant combination in terms of transcript level. This study describes an optimized cryopreservation protocol for Solea senegalensis sperm demonstrating for the first time that transcript degradation is the most sensitive predictor of cell status in this species after cryopreservation. |
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Solea senegalensis sperm cryopreservation: new insights on sperm qualityTrout oncorhynchus mykissEarly embryo developmentRainbow troutDna damageGene expressionGlutathione peroxidase-1Cyprinus carpioMessenger rnasCommon carpL. SpermCryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough information to identify specific damage caused by cryopreservation process (freezing and thawing). Specific tests, including lipid peroxidation and DNA damage, should be performed. In the present study, motility and lipid peroxidation were performed as specific tests allowing us to discard cryopreservation conditions such as methanol as internal cryoprotectant and bovine serum albumin as external cryoprotectant. In addition, a caspase 3/7 detection by flow cytometry was performed to analyze apoptosis activity in the best selected conditions. Moreover, new highly sensitive tests based on transcript number detection have recently been described in fish sperm cryopreservation. For this reason, a transcript level detection assay was performed on certain oxidative and chaperone genes related to fertilization ability and embryo development (hsp70, hsp90BB, hsp90AA, gpx) to select the best cryopreservation conditions. DMSO+ egg yolk proved to be the best cryoprotectant combination in terms of transcript level. This study describes an optimized cryopreservation protocol for Solea senegalensis sperm demonstrating for the first time that transcript degradation is the most sensitive predictor of cell status in this species after cryopreservation.PROMAR Program [REPLING 31-03-05-FEP-69, KLING 31-03-05-FEP-73]; COST action (Food and Agriculture) AQUAGAMETE [FA1205]; Fundagao para a Ciencia e Tecnologia (FCT, Portugal) [CCMAR/Multi/04326/2013]; REPLING project [CCMAR/SC/BPD/09/2016]; FCT postdoctoral fellowship [SFRH/BPD/63933/2009]Public Library ScienceSapientiaF RIESCO, MARTAOliveira, CatarinaSoares, FlorbelaGavaia, Paulo J.Dinis, Maria TeresaCabrita, Elsa2018-12-07T14:58:08Z2017-102017-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/11872eng1932-620310.1371/journal.pone.0186542info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:23:45Zoai:sapientia.ualg.pt:10400.1/11872Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:03:18.898341Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Solea senegalensis sperm cryopreservation: new insights on sperm quality |
title |
Solea senegalensis sperm cryopreservation: new insights on sperm quality |
spellingShingle |
Solea senegalensis sperm cryopreservation: new insights on sperm quality F RIESCO, MARTA Trout oncorhynchus mykiss Early embryo development Rainbow trout Dna damage Gene expression Glutathione peroxidase-1 Cyprinus carpio Messenger rnas Common carp L. Sperm |
title_short |
Solea senegalensis sperm cryopreservation: new insights on sperm quality |
title_full |
Solea senegalensis sperm cryopreservation: new insights on sperm quality |
title_fullStr |
Solea senegalensis sperm cryopreservation: new insights on sperm quality |
title_full_unstemmed |
Solea senegalensis sperm cryopreservation: new insights on sperm quality |
title_sort |
Solea senegalensis sperm cryopreservation: new insights on sperm quality |
author |
F RIESCO, MARTA |
author_facet |
F RIESCO, MARTA Oliveira, Catarina Soares, Florbela Gavaia, Paulo J. Dinis, Maria Teresa Cabrita, Elsa |
author_role |
author |
author2 |
Oliveira, Catarina Soares, Florbela Gavaia, Paulo J. Dinis, Maria Teresa Cabrita, Elsa |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
F RIESCO, MARTA Oliveira, Catarina Soares, Florbela Gavaia, Paulo J. Dinis, Maria Teresa Cabrita, Elsa |
dc.subject.por.fl_str_mv |
Trout oncorhynchus mykiss Early embryo development Rainbow trout Dna damage Gene expression Glutathione peroxidase-1 Cyprinus carpio Messenger rnas Common carp L. Sperm |
topic |
Trout oncorhynchus mykiss Early embryo development Rainbow trout Dna damage Gene expression Glutathione peroxidase-1 Cyprinus carpio Messenger rnas Common carp L. Sperm |
description |
Cryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough information to identify specific damage caused by cryopreservation process (freezing and thawing). Specific tests, including lipid peroxidation and DNA damage, should be performed. In the present study, motility and lipid peroxidation were performed as specific tests allowing us to discard cryopreservation conditions such as methanol as internal cryoprotectant and bovine serum albumin as external cryoprotectant. In addition, a caspase 3/7 detection by flow cytometry was performed to analyze apoptosis activity in the best selected conditions. Moreover, new highly sensitive tests based on transcript number detection have recently been described in fish sperm cryopreservation. For this reason, a transcript level detection assay was performed on certain oxidative and chaperone genes related to fertilization ability and embryo development (hsp70, hsp90BB, hsp90AA, gpx) to select the best cryopreservation conditions. DMSO+ egg yolk proved to be the best cryoprotectant combination in terms of transcript level. This study describes an optimized cryopreservation protocol for Solea senegalensis sperm demonstrating for the first time that transcript degradation is the most sensitive predictor of cell status in this species after cryopreservation. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10 2017-10-01T00:00:00Z 2018-12-07T14:58:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/11872 |
url |
http://hdl.handle.net/10400.1/11872 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1932-6203 10.1371/journal.pone.0186542 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133267179339776 |