Species detection from aquatic eDNA: assessing the importance of capture methods
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/21417 |
Resumo: | Original Research |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Species detection from aquatic eDNA: assessing the importance of capture methodsamphibians,capture methodsenvironmental DNAPCR replication thresholdsSalamandra salamandraOriginal ResearchEnvironmental DNA (eDNA) is increasingly used for biodiversity monitoring, particularly in aquatic systems. However, each step, from sample collection to bioinformatic analysis, can introduce biases and influence the reliability of results. While much effort has been put into the optimization of laboratory methods, less attention has been devoted to estimate the impacts of eDNA capture methods. To address this issue, water samples were collected at nine small ponds and puddles where up to 10 amphibian species occur, using precipitation, disc filters, and capsules. We focused on targeted detection of an amphibian species, Salamandra salamandra, and on the composition of the whole amphibian community. Species detection was performed using a novel qPCR assay for S. salamandra and high-throughput sequencing, combined with stringent versus relaxed PCR replication thresholds. Filtration techniques (disc filters and capsules) outperformed precipitation, generating a higher number of detections of S. salamandra and higher amounts of captured eDNA, while species detection was identical between disc filters and capsules. There were no significant differences between capture methods regarding amphibian community composition. The variation in detection success associated with capture methods was far higher than that associated with PCR replication, regardless of the detection method used. Our results highlight the importance of choosing a suitable capture method for eDNA studies and suggest that the choice of capture method outweighs the choice of detection method used. To the best of our knowledge, this is the first study to compare high-capacity capsules with common eDNA methods for water samples, such as precipitation and standard disc filtersWileyRepositório da Universidade de LisboaPeixoto, SaraChaves, CátiaVelo-Antón, GuillermoBeja, PedroEgeter, Bastian2021-06-02T13:38:14Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/21417engEnvironmental DNA. 2021;3:435–44810.1002/edn3.130info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:50:52Zoai:www.repository.utl.pt:10400.5/21417Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:00.514280Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Species detection from aquatic eDNA: assessing the importance of capture methods |
title |
Species detection from aquatic eDNA: assessing the importance of capture methods |
spellingShingle |
Species detection from aquatic eDNA: assessing the importance of capture methods Peixoto, Sara amphibians, capture methods environmental DNA PCR replication thresholds Salamandra salamandra |
title_short |
Species detection from aquatic eDNA: assessing the importance of capture methods |
title_full |
Species detection from aquatic eDNA: assessing the importance of capture methods |
title_fullStr |
Species detection from aquatic eDNA: assessing the importance of capture methods |
title_full_unstemmed |
Species detection from aquatic eDNA: assessing the importance of capture methods |
title_sort |
Species detection from aquatic eDNA: assessing the importance of capture methods |
author |
Peixoto, Sara |
author_facet |
Peixoto, Sara Chaves, Cátia Velo-Antón, Guillermo Beja, Pedro Egeter, Bastian |
author_role |
author |
author2 |
Chaves, Cátia Velo-Antón, Guillermo Beja, Pedro Egeter, Bastian |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Peixoto, Sara Chaves, Cátia Velo-Antón, Guillermo Beja, Pedro Egeter, Bastian |
dc.subject.por.fl_str_mv |
amphibians, capture methods environmental DNA PCR replication thresholds Salamandra salamandra |
topic |
amphibians, capture methods environmental DNA PCR replication thresholds Salamandra salamandra |
description |
Original Research |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-02T13:38:14Z 2021 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/21417 |
url |
http://hdl.handle.net/10400.5/21417 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental DNA. 2021;3:435–448 10.1002/edn3.130 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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