Cytogenetics of the Brazilian Bolitoglossa paraensis (Unterstein, 1930) salamanders (Caudata, Plethodontidae)

Detalhes bibliográficos
Autor(a) principal: SILVA, Jéssica Barata da
Data de Publicação: 2014
Outros Autores: SUAREZ, Pablo, NAGAMACHI, Cleusa Yoshiko, CARTER, Timothy Frederick, PIECZARKA, Julio Cesar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFPA
Texto Completo: http://repositorio.ufpa.br/jspui/handle/2011/6872
Resumo: Plethodontid salamanders of genus Bolitoglossa constitute the largest and most diverse group of salamanders, including around 20% of living caudate species. Recent studies have indicated the occurrence of five recognized species in the Brazilian Amazon Rainforest. We present here the first cytogenetic data of a Brazilian salamander, which may prove to be a useful by contribution to the cytotaxonomy of the genus. Specimens were collected near the “type” locality (Utinga, Belém, PA, Brazil). Chromosomal preparations from duodenal epithelial cells and testes were subjected to Giemsa staining, C-banding and DAPI/CMA3 fluorochrome staining. All specimens showed a karyotype with 13 bi-armed chromosome pairs (2n = 26). Nucleolar Organizer Regions, evidenced by CMA3, were located distally on the long arm of pair 7 (7q). DAPI+ heterochromatin was predominantly centromeric, with some small pericentromeric bands. Although the C-banding patterns of other Bolitoglossa species are so far unknown, cytogenetic studies conducted in other Plethodontid salamanders have demonstrated that pericentromeric heterochromatin is a useful cytological marker for identifying interspecific homeologies. Species diversification is usually accompanied by chromosomal changes. Therefore, the cytogenetic characterization of Bolitoglossa populations from the middle and western Brazilian Amazon Basin could identify differences which may lead to the identification of new species.
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spelling 2015-09-10T13:13:57Z2015-09-10T13:13:57Z2014-09SILVA, Jéssica Barata da et al. Cytogenetics of the Brazilian Bolitoglossa paraensis (Unterstein, 1930) salamanders (Caudata, Plethodontidae). Genetics and Molecular Biology, Ribeirão Preto, v. 37, n. 3, p. 526-529, set. 2014. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400008&lng=pt&nrm=iso>. Acesso em: 26 ago. 2015. <http://dx.doi.org/10.1590/S1415-47572014000400008>.1415-4757http://repositorio.ufpa.br/jspui/handle/2011/6872Plethodontid salamanders of genus Bolitoglossa constitute the largest and most diverse group of salamanders, including around 20% of living caudate species. Recent studies have indicated the occurrence of five recognized species in the Brazilian Amazon Rainforest. We present here the first cytogenetic data of a Brazilian salamander, which may prove to be a useful by contribution to the cytotaxonomy of the genus. Specimens were collected near the “type” locality (Utinga, Belém, PA, Brazil). Chromosomal preparations from duodenal epithelial cells and testes were subjected to Giemsa staining, C-banding and DAPI/CMA3 fluorochrome staining. All specimens showed a karyotype with 13 bi-armed chromosome pairs (2n = 26). Nucleolar Organizer Regions, evidenced by CMA3, were located distally on the long arm of pair 7 (7q). DAPI+ heterochromatin was predominantly centromeric, with some small pericentromeric bands. Although the C-banding patterns of other Bolitoglossa species are so far unknown, cytogenetic studies conducted in other Plethodontid salamanders have demonstrated that pericentromeric heterochromatin is a useful cytological marker for identifying interspecific homeologies. Species diversification is usually accompanied by chromosomal changes. Therefore, the cytogenetic characterization of Bolitoglossa populations from the middle and western Brazilian Amazon Basin could identify differences which may lead to the identification of new species.engAnfíbioSalamandrasCitogenéticaAmazônia brasileiraCytogenetics of the Brazilian Bolitoglossa paraensis (Unterstein, 1930) salamanders (Caudata, Plethodontidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSILVA, Jéssica Barata daSUAREZ, PabloNAGAMACHI, Cleusa YoshikoCARTER, Timothy FrederickPIECZARKA, Julio Cesarinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPAORIGINALArtigo_CytogeneticsBrazilianBolitoglossa.pdfArtigo_CytogeneticsBrazilianBolitoglossa.pdfapplication/pdf1177843http://repositorio.ufpa.br/oai/bitstream/2011/6872/1/Artigo_CytogeneticsBrazilianBolitoglossa.pdf921f351ebff75af0f8ffab8487ed7f94MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.ufpa.br/oai/bitstream/2011/6872/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-821328http://repositorio.ufpa.br/oai/bitstream/2011/6872/3/license_text1ddceb2a2635a9472b3c10d731d0906eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-822190http://repositorio.ufpa.br/oai/bitstream/2011/6872/4/license_rdf19e8a2b57ef43c09f4d7071d2153c97dMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81703http://repositorio.ufpa.br/oai/bitstream/2011/6872/5/license.txta12ee01655d4f43dacf016d5e6168febMD55TEXTArtigo_CytogeneticsBrazilianBolitoglossa.pdf.txtArtigo_CytogeneticsBrazilianBolitoglossa.pdf.txtExtracted texttext/plain17000http://repositorio.ufpa.br/oai/bitstream/2011/6872/6/Artigo_CytogeneticsBrazilianBolitoglossa.pdf.txt3792b8a76fcdc41823629562683545c0MD562011/68722019-05-23 08:11:48.359oai:repositorio.ufpa.br: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Repositório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232019-05-23T11:11:48Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false
dc.title.pt_BR.fl_str_mv Cytogenetics of the Brazilian Bolitoglossa paraensis (Unterstein, 1930) salamanders (Caudata, Plethodontidae)
title Cytogenetics of the Brazilian Bolitoglossa paraensis (Unterstein, 1930) salamanders (Caudata, Plethodontidae)
spellingShingle Cytogenetics of the Brazilian Bolitoglossa paraensis (Unterstein, 1930) salamanders (Caudata, Plethodontidae)
SILVA, Jéssica Barata da
Anfíbio
Salamandras
Citogenética
Amazônia brasileira
title_short Cytogenetics of the Brazilian Bolitoglossa paraensis (Unterstein, 1930) salamanders (Caudata, Plethodontidae)
title_full Cytogenetics of the Brazilian Bolitoglossa paraensis (Unterstein, 1930) salamanders (Caudata, Plethodontidae)
title_fullStr Cytogenetics of the Brazilian Bolitoglossa paraensis (Unterstein, 1930) salamanders (Caudata, Plethodontidae)
title_full_unstemmed Cytogenetics of the Brazilian Bolitoglossa paraensis (Unterstein, 1930) salamanders (Caudata, Plethodontidae)
title_sort Cytogenetics of the Brazilian Bolitoglossa paraensis (Unterstein, 1930) salamanders (Caudata, Plethodontidae)
author SILVA, Jéssica Barata da
author_facet SILVA, Jéssica Barata da
SUAREZ, Pablo
NAGAMACHI, Cleusa Yoshiko
CARTER, Timothy Frederick
PIECZARKA, Julio Cesar
author_role author
author2 SUAREZ, Pablo
NAGAMACHI, Cleusa Yoshiko
CARTER, Timothy Frederick
PIECZARKA, Julio Cesar
author2_role author
author
author
author
dc.contributor.author.fl_str_mv SILVA, Jéssica Barata da
SUAREZ, Pablo
NAGAMACHI, Cleusa Yoshiko
CARTER, Timothy Frederick
PIECZARKA, Julio Cesar
dc.subject.por.fl_str_mv Anfíbio
Salamandras
Citogenética
Amazônia brasileira
topic Anfíbio
Salamandras
Citogenética
Amazônia brasileira
description Plethodontid salamanders of genus Bolitoglossa constitute the largest and most diverse group of salamanders, including around 20% of living caudate species. Recent studies have indicated the occurrence of five recognized species in the Brazilian Amazon Rainforest. We present here the first cytogenetic data of a Brazilian salamander, which may prove to be a useful by contribution to the cytotaxonomy of the genus. Specimens were collected near the “type” locality (Utinga, Belém, PA, Brazil). Chromosomal preparations from duodenal epithelial cells and testes were subjected to Giemsa staining, C-banding and DAPI/CMA3 fluorochrome staining. All specimens showed a karyotype with 13 bi-armed chromosome pairs (2n = 26). Nucleolar Organizer Regions, evidenced by CMA3, were located distally on the long arm of pair 7 (7q). DAPI+ heterochromatin was predominantly centromeric, with some small pericentromeric bands. Although the C-banding patterns of other Bolitoglossa species are so far unknown, cytogenetic studies conducted in other Plethodontid salamanders have demonstrated that pericentromeric heterochromatin is a useful cytological marker for identifying interspecific homeologies. Species diversification is usually accompanied by chromosomal changes. Therefore, the cytogenetic characterization of Bolitoglossa populations from the middle and western Brazilian Amazon Basin could identify differences which may lead to the identification of new species.
publishDate 2014
dc.date.issued.fl_str_mv 2014-09
dc.date.accessioned.fl_str_mv 2015-09-10T13:13:57Z
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dc.identifier.citation.fl_str_mv SILVA, Jéssica Barata da et al. Cytogenetics of the Brazilian Bolitoglossa paraensis (Unterstein, 1930) salamanders (Caudata, Plethodontidae). Genetics and Molecular Biology, Ribeirão Preto, v. 37, n. 3, p. 526-529, set. 2014. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400008&lng=pt&nrm=iso>. Acesso em: 26 ago. 2015. <http://dx.doi.org/10.1590/S1415-47572014000400008>.
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identifier_str_mv SILVA, Jéssica Barata da et al. Cytogenetics of the Brazilian Bolitoglossa paraensis (Unterstein, 1930) salamanders (Caudata, Plethodontidae). Genetics and Molecular Biology, Ribeirão Preto, v. 37, n. 3, p. 526-529, set. 2014. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400008&lng=pt&nrm=iso>. Acesso em: 26 ago. 2015. <http://dx.doi.org/10.1590/S1415-47572014000400008>.
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