Size-selected silver clusters on polymer surfaces as plasmonic transducers for nano-biosensors
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/64178 |
Resumo: | The phenomenon of localized surface plasmon resonance (LSPR) was used for detection of proteins incubated on size-selected silver clusters embedded in poly (methyl methacrylate) (PMMA) layers. The silver clusters were produced by magnetron sputtering and subsequently sizeselected by electrostatic quadrupole mass spectrometer (EQMS). The size-selection process was followed by the deposition of silver clusters with low kinetic energies, i.e. in a soft-landing regime on PMMA, in order to avoid the deformation and/or fragmentation of the silver clusters. The role of PMMA hardness (viscosity) on cluster immersion was investigated to control the homogeneity in distribution of clusters, which can be partly or fully embedded in PMMA. The viscosity of the polymers was found to be an essential part in the immersion of the silver nanoparticles into the polymer, which can be controlled through tuning of its viscosity or hardness utilizing simple thermal annealing of the samples. The optical properties of the silver clusters - in particular the localized surface plasmon resonance (LSPR) – were measured with the optical absorption spectra of the silver clusters, in which was possible to clearly identify an intensity and a resonance band of the deposited clusters. Proteins of interest were incubated on the clusters utilizing antibody-antigen scheme and clear change in the parameters of the LSPR band was observed allowing protein detection and demonstrating applicability of the fabricated composites in bio-sensing. |
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7160 |
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Size-selected silver clusters on polymer surfaces as plasmonic transducers for nano-biosensorsOptical nano-biosensorslocalized plasmon resonanceDomínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e TecnologiasThe phenomenon of localized surface plasmon resonance (LSPR) was used for detection of proteins incubated on size-selected silver clusters embedded in poly (methyl methacrylate) (PMMA) layers. The silver clusters were produced by magnetron sputtering and subsequently sizeselected by electrostatic quadrupole mass spectrometer (EQMS). The size-selection process was followed by the deposition of silver clusters with low kinetic energies, i.e. in a soft-landing regime on PMMA, in order to avoid the deformation and/or fragmentation of the silver clusters. The role of PMMA hardness (viscosity) on cluster immersion was investigated to control the homogeneity in distribution of clusters, which can be partly or fully embedded in PMMA. The viscosity of the polymers was found to be an essential part in the immersion of the silver nanoparticles into the polymer, which can be controlled through tuning of its viscosity or hardness utilizing simple thermal annealing of the samples. The optical properties of the silver clusters - in particular the localized surface plasmon resonance (LSPR) – were measured with the optical absorption spectra of the silver clusters, in which was possible to clearly identify an intensity and a resonance band of the deposited clusters. Proteins of interest were incubated on the clusters utilizing antibody-antigen scheme and clear change in the parameters of the LSPR band was observed allowing protein detection and demonstrating applicability of the fabricated composites in bio-sensing.Silva, AnaRUNBrasil, Francisco Maria Cristão Marques Luquet2019-03-22T11:34:48Z2017-0620172017-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/64178enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:30:30Zoai:run.unl.pt:10362/64178Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:34:06.214163Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Size-selected silver clusters on polymer surfaces as plasmonic transducers for nano-biosensors |
title |
Size-selected silver clusters on polymer surfaces as plasmonic transducers for nano-biosensors |
spellingShingle |
Size-selected silver clusters on polymer surfaces as plasmonic transducers for nano-biosensors Brasil, Francisco Maria Cristão Marques Luquet Optical nano-biosensors localized plasmon resonance Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
title_short |
Size-selected silver clusters on polymer surfaces as plasmonic transducers for nano-biosensors |
title_full |
Size-selected silver clusters on polymer surfaces as plasmonic transducers for nano-biosensors |
title_fullStr |
Size-selected silver clusters on polymer surfaces as plasmonic transducers for nano-biosensors |
title_full_unstemmed |
Size-selected silver clusters on polymer surfaces as plasmonic transducers for nano-biosensors |
title_sort |
Size-selected silver clusters on polymer surfaces as plasmonic transducers for nano-biosensors |
author |
Brasil, Francisco Maria Cristão Marques Luquet |
author_facet |
Brasil, Francisco Maria Cristão Marques Luquet |
author_role |
author |
dc.contributor.none.fl_str_mv |
Silva, Ana RUN |
dc.contributor.author.fl_str_mv |
Brasil, Francisco Maria Cristão Marques Luquet |
dc.subject.por.fl_str_mv |
Optical nano-biosensors localized plasmon resonance Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
topic |
Optical nano-biosensors localized plasmon resonance Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
description |
The phenomenon of localized surface plasmon resonance (LSPR) was used for detection of proteins incubated on size-selected silver clusters embedded in poly (methyl methacrylate) (PMMA) layers. The silver clusters were produced by magnetron sputtering and subsequently sizeselected by electrostatic quadrupole mass spectrometer (EQMS). The size-selection process was followed by the deposition of silver clusters with low kinetic energies, i.e. in a soft-landing regime on PMMA, in order to avoid the deformation and/or fragmentation of the silver clusters. The role of PMMA hardness (viscosity) on cluster immersion was investigated to control the homogeneity in distribution of clusters, which can be partly or fully embedded in PMMA. The viscosity of the polymers was found to be an essential part in the immersion of the silver nanoparticles into the polymer, which can be controlled through tuning of its viscosity or hardness utilizing simple thermal annealing of the samples. The optical properties of the silver clusters - in particular the localized surface plasmon resonance (LSPR) – were measured with the optical absorption spectra of the silver clusters, in which was possible to clearly identify an intensity and a resonance band of the deposited clusters. Proteins of interest were incubated on the clusters utilizing antibody-antigen scheme and clear change in the parameters of the LSPR band was observed allowing protein detection and demonstrating applicability of the fabricated composites in bio-sensing. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06 2017 2017-06-01T00:00:00Z 2019-03-22T11:34:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/64178 |
url |
http://hdl.handle.net/10362/64178 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137962324131840 |