A novel antimicrobial lectin from Eugenia malaccensis that stimulates cutaneous healing in mice model

Detalhes bibliográficos
Autor(a) principal: Brustein, V. P.
Data de Publicação: 2012
Outros Autores: Souza-Araújo, F. V., Vaz, A. F. M., Araújo, R. V. S., Paiva, P. M. G., Coelho, L. C. B. B., Carneiro-Leão, A. M. A., Teixeira, J. A., Carneiro-da-Cunha, M. G., Correia, M. T. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/56437
Resumo: Objective The present work reports the purification and partial characterization of an antibacterial lectin (EmaL) obtained from Eugenia malaccensis seeds as well as the evaluation of its effect in the daily topical treatment of repairing process of cutaneous wounds in mice. Materials and methods The cutaneous wound was produced by the incision of the skin and use of lectin in the treatment of mice cutaneous wounds was evaluated. Surgical wounds were treated daily with a topical administration of EmaL and parameters such as edema, hyperemia, scab, granulation and scar tissues as well as contraction of wounds were analyzed. Results A novel lectin, with a molecular mass of 14 kDa, was isolated from E. malaccensis using affinity chromatography. The lectin (EmaL) agglutinated glutaraldehyde-treated rabbit and human erythrocytes; the lectin-induced rabbit erythrocyte agglutination was inhibited by glucose, casein, ovalbumin and fetuin. Also, Emal was very effective in the inhibition of bacterial growth, with the best inhibition results obtained for Staphylococcus aureus. Inflammatory signals such as edema and hyperemia were statistically less intense when EmaL was applied compared to the control. The histopathological analysis showed that the treated injured tissue presented reepithelialization (complete or partial) and areas of transition more evidenced than those of the control group, especially due to well organized pattern of collagen fibers presented in the granulation fibrous tissue. Conclusion Presented results are a preliminary indication of the pharmacological interest in using EmaL as antimicrobial agent and in the repairing process of cutaneous wounds.
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spelling A novel antimicrobial lectin from Eugenia malaccensis that stimulates cutaneous healing in mice modelAntibacterialCutaneous wound healingEugenia malaccensisLectinObjective The present work reports the purification and partial characterization of an antibacterial lectin (EmaL) obtained from Eugenia malaccensis seeds as well as the evaluation of its effect in the daily topical treatment of repairing process of cutaneous wounds in mice. Materials and methods The cutaneous wound was produced by the incision of the skin and use of lectin in the treatment of mice cutaneous wounds was evaluated. Surgical wounds were treated daily with a topical administration of EmaL and parameters such as edema, hyperemia, scab, granulation and scar tissues as well as contraction of wounds were analyzed. Results A novel lectin, with a molecular mass of 14 kDa, was isolated from E. malaccensis using affinity chromatography. The lectin (EmaL) agglutinated glutaraldehyde-treated rabbit and human erythrocytes; the lectin-induced rabbit erythrocyte agglutination was inhibited by glucose, casein, ovalbumin and fetuin. Also, Emal was very effective in the inhibition of bacterial growth, with the best inhibition results obtained for Staphylococcus aureus. Inflammatory signals such as edema and hyperemia were statistically less intense when EmaL was applied compared to the control. The histopathological analysis showed that the treated injured tissue presented reepithelialization (complete or partial) and areas of transition more evidenced than those of the control group, especially due to well organized pattern of collagen fibers presented in the granulation fibrous tissue. Conclusion Presented results are a preliminary indication of the pharmacological interest in using EmaL as antimicrobial agent and in the repairing process of cutaneous wounds.This paper was financially supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), FACEPE and CAPES, Brazil. The authors are deeply grateful for the technical assistance of Maria Barbosa Reis da Silva and João Antonio Virgínio and Alfa/VALNATURA Project.info:eu-repo/semantics/publishedVersionSpringer NatureUniversidade do MinhoBrustein, V. P.Souza-Araújo, F. V.Vaz, A. F. M.Araújo, R. V. S.Paiva, P. M. G.Coelho, L. C. B. B.Carneiro-Leão, A. M. A.Teixeira, J. A.Carneiro-da-Cunha, M. G.Correia, M. T. S.2012-122012-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/56437engBrustein, V. P.; Souza-Araújo, F. V.; Vaz, A. F. M.; Araújo, R. V. S.; Paiva, P. M. G.; Coelho, L. C. B. B.; Carneiro-Leão, A. M. A.; Teixeira, José A.; Carneiro-da-Cunha, M. G.; Correia, M. T. S., A novel antimicrobial lectin from Eugenia malaccensis that stimulates cutaneous healing in mice model. Inflammopharmacology, 20(6), 315-322, 20120925-46921568-560810.1007/s10787-011-0113-522271003https://www.springer.com/biomed/pharmacology+%26+toxicology/journal/10787info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:06:35Zoai:repositorium.sdum.uminho.pt:1822/56437Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:57:17.356777Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A novel antimicrobial lectin from Eugenia malaccensis that stimulates cutaneous healing in mice model
title A novel antimicrobial lectin from Eugenia malaccensis that stimulates cutaneous healing in mice model
spellingShingle A novel antimicrobial lectin from Eugenia malaccensis that stimulates cutaneous healing in mice model
Brustein, V. P.
Antibacterial
Cutaneous wound healing
Eugenia malaccensis
Lectin
title_short A novel antimicrobial lectin from Eugenia malaccensis that stimulates cutaneous healing in mice model
title_full A novel antimicrobial lectin from Eugenia malaccensis that stimulates cutaneous healing in mice model
title_fullStr A novel antimicrobial lectin from Eugenia malaccensis that stimulates cutaneous healing in mice model
title_full_unstemmed A novel antimicrobial lectin from Eugenia malaccensis that stimulates cutaneous healing in mice model
title_sort A novel antimicrobial lectin from Eugenia malaccensis that stimulates cutaneous healing in mice model
author Brustein, V. P.
author_facet Brustein, V. P.
Souza-Araújo, F. V.
Vaz, A. F. M.
Araújo, R. V. S.
Paiva, P. M. G.
Coelho, L. C. B. B.
Carneiro-Leão, A. M. A.
Teixeira, J. A.
Carneiro-da-Cunha, M. G.
Correia, M. T. S.
author_role author
author2 Souza-Araújo, F. V.
Vaz, A. F. M.
Araújo, R. V. S.
Paiva, P. M. G.
Coelho, L. C. B. B.
Carneiro-Leão, A. M. A.
Teixeira, J. A.
Carneiro-da-Cunha, M. G.
Correia, M. T. S.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Brustein, V. P.
Souza-Araújo, F. V.
Vaz, A. F. M.
Araújo, R. V. S.
Paiva, P. M. G.
Coelho, L. C. B. B.
Carneiro-Leão, A. M. A.
Teixeira, J. A.
Carneiro-da-Cunha, M. G.
Correia, M. T. S.
dc.subject.por.fl_str_mv Antibacterial
Cutaneous wound healing
Eugenia malaccensis
Lectin
topic Antibacterial
Cutaneous wound healing
Eugenia malaccensis
Lectin
description Objective The present work reports the purification and partial characterization of an antibacterial lectin (EmaL) obtained from Eugenia malaccensis seeds as well as the evaluation of its effect in the daily topical treatment of repairing process of cutaneous wounds in mice. Materials and methods The cutaneous wound was produced by the incision of the skin and use of lectin in the treatment of mice cutaneous wounds was evaluated. Surgical wounds were treated daily with a topical administration of EmaL and parameters such as edema, hyperemia, scab, granulation and scar tissues as well as contraction of wounds were analyzed. Results A novel lectin, with a molecular mass of 14 kDa, was isolated from E. malaccensis using affinity chromatography. The lectin (EmaL) agglutinated glutaraldehyde-treated rabbit and human erythrocytes; the lectin-induced rabbit erythrocyte agglutination was inhibited by glucose, casein, ovalbumin and fetuin. Also, Emal was very effective in the inhibition of bacterial growth, with the best inhibition results obtained for Staphylococcus aureus. Inflammatory signals such as edema and hyperemia were statistically less intense when EmaL was applied compared to the control. The histopathological analysis showed that the treated injured tissue presented reepithelialization (complete or partial) and areas of transition more evidenced than those of the control group, especially due to well organized pattern of collagen fibers presented in the granulation fibrous tissue. Conclusion Presented results are a preliminary indication of the pharmacological interest in using EmaL as antimicrobial agent and in the repairing process of cutaneous wounds.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
2012-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/56437
url http://hdl.handle.net/1822/56437
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brustein, V. P.; Souza-Araújo, F. V.; Vaz, A. F. M.; Araújo, R. V. S.; Paiva, P. M. G.; Coelho, L. C. B. B.; Carneiro-Leão, A. M. A.; Teixeira, José A.; Carneiro-da-Cunha, M. G.; Correia, M. T. S., A novel antimicrobial lectin from Eugenia malaccensis that stimulates cutaneous healing in mice model. Inflammopharmacology, 20(6), 315-322, 2012
0925-4692
1568-5608
10.1007/s10787-011-0113-5
22271003
https://www.springer.com/biomed/pharmacology+%26+toxicology/journal/10787
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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