A Quasi-Domesticate Relic Hybrid Population of Saccharomyces cerevisiae × S. Paradoxus Adapted to Olive Brine

Detalhes bibliográficos
Autor(a) principal: Pontes, Ana
Data de Publicação: 2019
Outros Autores: Čadež, Neža, Gonçalves, Paula, Sampaio, José Paulo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/104462
Resumo: Fundacao para a Ciencia e a Tecnologia (Portugal) grants PTDC/BIA-MIC/30785/2017. SFRH/BD/136462/2018. Slovenian Research Agency Grants P4-0116 and MRIC-ULZIM, IP-0510 (NC).
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spelling A Quasi-Domesticate Relic Hybrid Population of Saccharomyces cerevisiae × S. Paradoxus Adapted to Olive BrineHybridizationMicrobe population genomicsMicrobiology of olive brineSaccharomyces cerevisiaeYeastMolecular MedicineGeneticsGenetics(clinical)Fundacao para a Ciencia e a Tecnologia (Portugal) grants PTDC/BIA-MIC/30785/2017. SFRH/BD/136462/2018. Slovenian Research Agency Grants P4-0116 and MRIC-ULZIM, IP-0510 (NC).The adaptation of the yeast Saccharomyces cerevisiae to man-made environments for the fermentation of foodstuffs and beverages illustrates the scientific, social, and economic relevance of microbe domestication. Here we address a yet unexplored aspect of S. cerevisiae domestication, that of the emergence of lineages harboring some domestication signatures but that do not fit completely in the archetype of a domesticated yeast, by studying S. cerevisiae strains associated with processed olives, namely table olives, olive brine, olive oil, and alpechin. We confirmed earlier observations that reported that the Olives population results from a hybridization between S. cerevisiae and S. paradoxus. We concluded that the olive hybrids form a monophyletic lineage and that the S. cerevisiae progenitor belonged to the wine population of this species. We propose that homoploid hybridization gave rise to a diploid hybrid genome, which subsequently underwent the loss of most of the S. paradoxus sub-genome. Such a massive loss of heterozygosity was probably driven by adaptation to the new niche. We observed that olive strains are more fit to grow and survive in olive brine than control S. cerevisiae wine strains and that they appear to be adapted to cope with the presence of NaCl in olive brine through expansion of copy number of ENA genes. We also investigated whether the S. paradoxus HXT alleles retained by the Olives population were likely to contribute to the observed superior ability of these strains to consume sugars in brine. Our experiments indicate that sugar consumption profiles in the presence of NaCl are different between members of the Olives and Wine populations and only when cells are cultivated in nutritional conditions that support adaptation of their proteome to the high salt environment, which suggests that the observed differences are due to a better overall fitness of olives strains in the presence of high NaCl concentrations. Although relic olive hybrids exhibit several characteristics of a domesticated lineage, tangible benefits to humans cannot be associated with their phenotypes. These strains can be seen as a case of adaptation without positive or negative consequences to humans, that we define as a quasi-domestication.DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitRUNPontes, AnaČadež, NežaGonçalves, PaulaSampaio, José Paulo2020-09-21T22:16:41Z2019-05-292019-05-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/104462eng1664-8021PURE: 18785233https://doi.org/10.3389/fgene.2019.00449info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:49:58Zoai:run.unl.pt:10362/104462Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:40:14.015085Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A Quasi-Domesticate Relic Hybrid Population of Saccharomyces cerevisiae × S. Paradoxus Adapted to Olive Brine
title A Quasi-Domesticate Relic Hybrid Population of Saccharomyces cerevisiae × S. Paradoxus Adapted to Olive Brine
spellingShingle A Quasi-Domesticate Relic Hybrid Population of Saccharomyces cerevisiae × S. Paradoxus Adapted to Olive Brine
Pontes, Ana
Hybridization
Microbe population genomics
Microbiology of olive brine
Saccharomyces cerevisiae
Yeast
Molecular Medicine
Genetics
Genetics(clinical)
title_short A Quasi-Domesticate Relic Hybrid Population of Saccharomyces cerevisiae × S. Paradoxus Adapted to Olive Brine
title_full A Quasi-Domesticate Relic Hybrid Population of Saccharomyces cerevisiae × S. Paradoxus Adapted to Olive Brine
title_fullStr A Quasi-Domesticate Relic Hybrid Population of Saccharomyces cerevisiae × S. Paradoxus Adapted to Olive Brine
title_full_unstemmed A Quasi-Domesticate Relic Hybrid Population of Saccharomyces cerevisiae × S. Paradoxus Adapted to Olive Brine
title_sort A Quasi-Domesticate Relic Hybrid Population of Saccharomyces cerevisiae × S. Paradoxus Adapted to Olive Brine
author Pontes, Ana
author_facet Pontes, Ana
Čadež, Neža
Gonçalves, Paula
Sampaio, José Paulo
author_role author
author2 Čadež, Neža
Gonçalves, Paula
Sampaio, José Paulo
author2_role author
author
author
dc.contributor.none.fl_str_mv DCV - Departamento de Ciências da Vida
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Pontes, Ana
Čadež, Neža
Gonçalves, Paula
Sampaio, José Paulo
dc.subject.por.fl_str_mv Hybridization
Microbe population genomics
Microbiology of olive brine
Saccharomyces cerevisiae
Yeast
Molecular Medicine
Genetics
Genetics(clinical)
topic Hybridization
Microbe population genomics
Microbiology of olive brine
Saccharomyces cerevisiae
Yeast
Molecular Medicine
Genetics
Genetics(clinical)
description Fundacao para a Ciencia e a Tecnologia (Portugal) grants PTDC/BIA-MIC/30785/2017. SFRH/BD/136462/2018. Slovenian Research Agency Grants P4-0116 and MRIC-ULZIM, IP-0510 (NC).
publishDate 2019
dc.date.none.fl_str_mv 2019-05-29
2019-05-29T00:00:00Z
2020-09-21T22:16:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/104462
url http://hdl.handle.net/10362/104462
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1664-8021
PURE: 18785233
https://doi.org/10.3389/fgene.2019.00449
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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