Cerebrospinal fluid chitinases as biomarkers for Amyotrophic Lateral Sclerosis

Detalhes bibliográficos
Autor(a) principal: Costa, Julia
Data de Publicação: 2021
Outros Autores: Gromicho, Marta, Pronto Laborinho, Ana Catarina, Almeida, Conceição, Gomes, Ricardo A., Guerreiro, Ana C. L., Oliva, Abel, Pinto, Susana, Carvalho, Mamede
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/49468
Resumo: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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spelling Cerebrospinal fluid chitinases as biomarkers for Amyotrophic Lateral SclerosisAmyotrophic lateral sclerosisBiomarkersCerebrospinal fluidChitinases© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Amyotrophic lateral sclerosis (ALS) is a neurodegenerative neuromuscular disease that affects motor neurons controlling voluntary muscles. Survival is usually 2-5 years after onset, and death occurs due to respiratory failure. The identification of biomarkers would be very useful to help in disease diagnosis and for patient stratification based on, e.g., progression rate, with implications in therapeutic trials. Neurofilaments constitute already-promising markers for ALS and, recently, chitinases have emerged as novel marker targets for the disease. Here, we investigated cerebrospinal fluid (CSF) chitinases as potential markers for ALS. Chitotriosidase (CHIT1), chitinase-3-like protein 1 (CHI3L1), chitinase-3-like protein 2 (CHI3L2) and the benchmark marker phosphoneurofilament heavy chain (pNFH) were quantified by an enzyme-linked immunosorbent assay (ELISA) from the CSF of 34 ALS patients and 24 control patients with other neurological diseases. CSF was also analyzed by UHPLC-mass spectrometry. All three chitinases, as well as pNFH, were found to correlate with disease progression rate. Furthermore, CHIT1 was elevated in ALS patients with high diagnostic performance, as was pNFH. On the other hand, CHIT1 correlated with forced vital capacity (FVC). The three chitinases correlated with pNFH, indicating a relation between degeneration and neuroinflammation. In conclusion, our results supported the value of CHIT1 as a diagnostic and progression rate biomarker, and its potential as respiratory function marker. The results opened novel perspectives to explore chitinases as biomarkers and their functional relevance in ALS.We acknowledge iNOVA4Health – UIDB/04462/2020 and UIDP/04462/2020, a program financially supported by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior, through national funds.MDPIRepositório da Universidade de LisboaCosta, JuliaGromicho, MartaPronto Laborinho, Ana CatarinaAlmeida, ConceiçãoGomes, Ricardo A.Guerreiro, Ana C. L.Oliva, AbelPinto, SusanaCarvalho, Mamede2021-09-08T14:27:57Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/49468engDiagnostics (Basel). 2021 Jul 5;11(7):121010.3390/diagnostics110712102075-4418info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:53:16Zoai:repositorio.ul.pt:10451/49468Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:01:06.286605Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cerebrospinal fluid chitinases as biomarkers for Amyotrophic Lateral Sclerosis
title Cerebrospinal fluid chitinases as biomarkers for Amyotrophic Lateral Sclerosis
spellingShingle Cerebrospinal fluid chitinases as biomarkers for Amyotrophic Lateral Sclerosis
Costa, Julia
Amyotrophic lateral sclerosis
Biomarkers
Cerebrospinal fluid
Chitinases
title_short Cerebrospinal fluid chitinases as biomarkers for Amyotrophic Lateral Sclerosis
title_full Cerebrospinal fluid chitinases as biomarkers for Amyotrophic Lateral Sclerosis
title_fullStr Cerebrospinal fluid chitinases as biomarkers for Amyotrophic Lateral Sclerosis
title_full_unstemmed Cerebrospinal fluid chitinases as biomarkers for Amyotrophic Lateral Sclerosis
title_sort Cerebrospinal fluid chitinases as biomarkers for Amyotrophic Lateral Sclerosis
author Costa, Julia
author_facet Costa, Julia
Gromicho, Marta
Pronto Laborinho, Ana Catarina
Almeida, Conceição
Gomes, Ricardo A.
Guerreiro, Ana C. L.
Oliva, Abel
Pinto, Susana
Carvalho, Mamede
author_role author
author2 Gromicho, Marta
Pronto Laborinho, Ana Catarina
Almeida, Conceição
Gomes, Ricardo A.
Guerreiro, Ana C. L.
Oliva, Abel
Pinto, Susana
Carvalho, Mamede
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Costa, Julia
Gromicho, Marta
Pronto Laborinho, Ana Catarina
Almeida, Conceição
Gomes, Ricardo A.
Guerreiro, Ana C. L.
Oliva, Abel
Pinto, Susana
Carvalho, Mamede
dc.subject.por.fl_str_mv Amyotrophic lateral sclerosis
Biomarkers
Cerebrospinal fluid
Chitinases
topic Amyotrophic lateral sclerosis
Biomarkers
Cerebrospinal fluid
Chitinases
description © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
publishDate 2021
dc.date.none.fl_str_mv 2021-09-08T14:27:57Z
2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/49468
url http://hdl.handle.net/10451/49468
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Diagnostics (Basel). 2021 Jul 5;11(7):1210
10.3390/diagnostics11071210
2075-4418
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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