Formation of the posterior commissure in the developing zebrafish embryo

Detalhes bibliográficos
Autor(a) principal: Novais, Leonor Filipe Silva dos Reis
Data de Publicação: 2023
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/61549
Resumo: Tese de mestrado, Biologia Evolutiva e do Desenvolvimento, 2023, Universidade de Lisboa, Faculdade de Ciências
id RCAP_94ddf7e74f26b9e6b605651d8dcebc7f
oai_identifier_str oai:repositorio.ul.pt:10451/61549
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Formation of the posterior commissure in the developing zebrafish embryocircuitos neuronaisformação da comissurapeixe zebraTeses de mestrado - 2023Departamento de Biologia AnimalTese de mestrado, Biologia Evolutiva e do Desenvolvimento, 2023, Universidade de Lisboa, Faculdade de CiênciasWe aimed to study the development of the tract of the posterior commissure (TPC) in the early zebrafish embryo. Even though TPC anatomy has been described, its development has yet to be explored. The TPC is conserved in vertebrates and connects the right and left parts of the brain, in the diencephalonmesencephalon region. In the zebrafish, it has been linked to interhemispheric communication and binocular integration. The Lx200 transgenic line, created in the laboratory, was used, since it expresses fluorescent proteins in neuronal subpopulations, including in the TPC and the ventral caudal cluster (vcc), whose neurons project along this tract. We determined that the Lx200 transgenic line labels a subcluster of the vcc, the Lx200-vcc, with projections pioneering TPC development, at around 20 hpf. The location of the Lx200-vcc was determined as ventral, in the prosomere 1 (p1)-mesencephalon region, while proper TPC was located in the p1. In situ hybridization assays to observe the known expression pattern of genes allowed for this localization. At larval stages, the TPC location was similar, close to the dorsal midline in the p1 region, while somas were located in the p1- mesencephalon region, both were determined via photoconversion of the Dendra2 protein. Dendra2 photoconversion also allowed for the determination of Lx200-vcc cells' contribution to the tracts. Rostral Lx200-vcc cells project along the TPC, but not to the medial-longitudinal fascicle (MLF), while caudal Lx200-vcc cells likely contribute to the MLF. Whole mount in vivo lightsheet and confocal imaging allowed for a qualitative and quantitative description of the process of TPC formation. We utilised the Imaris image analysis software to trace and track the projections in time. TPC development occurred via non-turning, branching projections, in a ventral to dorsal direction. Connections occurred via the contact of opposing growth cones or by growth cone-axon connections. We provide a detailed characterization of the Lx200-vcc neurons and of TPC development. Our work contributes to a deeper understanding of axonogenesis, a major step in central nervous system development, in a whole embryo context. Future work will focus on the molecular pathways responsible for TPC development.Diez del Corral, RuthAmorim, Maria Clara C. F. Pessoa de, 1967-Repositório da Universidade de LisboaNovais, Leonor Filipe Silva dos Reis2023-12-27T16:08:03Z202320232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10451/61549enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-01T01:16:54Zoai:repositorio.ul.pt:10451/61549Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:57:02.744894Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Formation of the posterior commissure in the developing zebrafish embryo
title Formation of the posterior commissure in the developing zebrafish embryo
spellingShingle Formation of the posterior commissure in the developing zebrafish embryo
Novais, Leonor Filipe Silva dos Reis
circuitos neuronais
formação da comissura
peixe zebra
Teses de mestrado - 2023
Departamento de Biologia Animal
title_short Formation of the posterior commissure in the developing zebrafish embryo
title_full Formation of the posterior commissure in the developing zebrafish embryo
title_fullStr Formation of the posterior commissure in the developing zebrafish embryo
title_full_unstemmed Formation of the posterior commissure in the developing zebrafish embryo
title_sort Formation of the posterior commissure in the developing zebrafish embryo
author Novais, Leonor Filipe Silva dos Reis
author_facet Novais, Leonor Filipe Silva dos Reis
author_role author
dc.contributor.none.fl_str_mv Diez del Corral, Ruth
Amorim, Maria Clara C. F. Pessoa de, 1967-
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Novais, Leonor Filipe Silva dos Reis
dc.subject.por.fl_str_mv circuitos neuronais
formação da comissura
peixe zebra
Teses de mestrado - 2023
Departamento de Biologia Animal
topic circuitos neuronais
formação da comissura
peixe zebra
Teses de mestrado - 2023
Departamento de Biologia Animal
description Tese de mestrado, Biologia Evolutiva e do Desenvolvimento, 2023, Universidade de Lisboa, Faculdade de Ciências
publishDate 2023
dc.date.none.fl_str_mv 2023-12-27T16:08:03Z
2023
2023
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/61549
url http://hdl.handle.net/10451/61549
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799136454316654592

Registros relacionados