A multi-bioassay integrated approach to assess the antifouling potential of the cyanobacterial metabolites portoamides

Detalhes bibliográficos
Autor(a) principal: Antunes, J.
Data de Publicação: 2019
Outros Autores: Pereira, S., Ribeiro, T., Plowman, J.E., Thomas, A., Clerens, S., Campos, A., Vasconcelos, V., Almeida, J.R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/130450
Resumo: The cyclic peptides portoamides produced by the cyanobacterium Phormidium sp. LEGE 05292 were previously isolated and their ability to condition microcommunities by allelopathic effect was described. These interesting bioactive properties are, however, still underexplored as their biotechnological applications may be vast. This study aims to investigate the antifouling potential of portoamides, given that a challenge in the search for new environmentally friendly antifouling products is to find non-toxic natural alternatives with the ability to prevent colonization of different biofouling species, from bacteria to macroinvertebrates. A multi-bioassay approach was applied to assess portoamides antifouling properties, marine ecotoxicity and molecular mode of action. Results showed high effectiveness in the prevention of mussel larvae settlement (EC50 = 3.16 µM), and also bioactivity towards growth and biofilm disruption of marine biofouling bacterial strains, while not showing toxicity towards both target and non-target species. Antifouling molecular targets in mussel larvae include energy metabolism modifications (failure in proton-transporting ATPases activity), structural alterations of the gills and protein and gene regulatory mechanisms. Overall, portoamides reveal a broad-spectrum bioactivity towards diverse biofouling species, including a non-toxic and reversible effect towards mussel larvae, showing potential to be incorporated as an active ingredient in antifouling coatings.
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spelling A multi-bioassay integrated approach to assess the antifouling potential of the cyanobacterial metabolites portoamidesAntifoulingBiofoulingCyanobacteriaMarine biofilmsMode of actionNatural productsThe cyclic peptides portoamides produced by the cyanobacterium Phormidium sp. LEGE 05292 were previously isolated and their ability to condition microcommunities by allelopathic effect was described. These interesting bioactive properties are, however, still underexplored as their biotechnological applications may be vast. This study aims to investigate the antifouling potential of portoamides, given that a challenge in the search for new environmentally friendly antifouling products is to find non-toxic natural alternatives with the ability to prevent colonization of different biofouling species, from bacteria to macroinvertebrates. A multi-bioassay approach was applied to assess portoamides antifouling properties, marine ecotoxicity and molecular mode of action. Results showed high effectiveness in the prevention of mussel larvae settlement (EC50 = 3.16 µM), and also bioactivity towards growth and biofilm disruption of marine biofouling bacterial strains, while not showing toxicity towards both target and non-target species. Antifouling molecular targets in mussel larvae include energy metabolism modifications (failure in proton-transporting ATPases activity), structural alterations of the gills and protein and gene regulatory mechanisms. Overall, portoamides reveal a broad-spectrum bioactivity towards diverse biofouling species, including a non-toxic and reversible effect towards mussel larvae, showing potential to be incorporated as an active ingredient in antifouling coatings.MDPI20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/130450engISSN 1660-3397https://doi.org/10.3390/md17020111Antunes, J.Pereira, S.Ribeiro, T.Plowman, J.E.Thomas, A.Clerens, S.Campos, A.Vasconcelos, V.Almeida, J.R.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T12:53:58Zoai:repositorio-aberto.up.pt:10216/130450Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:28:58.350842Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A multi-bioassay integrated approach to assess the antifouling potential of the cyanobacterial metabolites portoamides
title A multi-bioassay integrated approach to assess the antifouling potential of the cyanobacterial metabolites portoamides
spellingShingle A multi-bioassay integrated approach to assess the antifouling potential of the cyanobacterial metabolites portoamides
Antunes, J.
Antifouling
Biofouling
Cyanobacteria
Marine biofilms
Mode of action
Natural products
title_short A multi-bioassay integrated approach to assess the antifouling potential of the cyanobacterial metabolites portoamides
title_full A multi-bioassay integrated approach to assess the antifouling potential of the cyanobacterial metabolites portoamides
title_fullStr A multi-bioassay integrated approach to assess the antifouling potential of the cyanobacterial metabolites portoamides
title_full_unstemmed A multi-bioassay integrated approach to assess the antifouling potential of the cyanobacterial metabolites portoamides
title_sort A multi-bioassay integrated approach to assess the antifouling potential of the cyanobacterial metabolites portoamides
author Antunes, J.
author_facet Antunes, J.
Pereira, S.
Ribeiro, T.
Plowman, J.E.
Thomas, A.
Clerens, S.
Campos, A.
Vasconcelos, V.
Almeida, J.R.
author_role author
author2 Pereira, S.
Ribeiro, T.
Plowman, J.E.
Thomas, A.
Clerens, S.
Campos, A.
Vasconcelos, V.
Almeida, J.R.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Antunes, J.
Pereira, S.
Ribeiro, T.
Plowman, J.E.
Thomas, A.
Clerens, S.
Campos, A.
Vasconcelos, V.
Almeida, J.R.
dc.subject.por.fl_str_mv Antifouling
Biofouling
Cyanobacteria
Marine biofilms
Mode of action
Natural products
topic Antifouling
Biofouling
Cyanobacteria
Marine biofilms
Mode of action
Natural products
description The cyclic peptides portoamides produced by the cyanobacterium Phormidium sp. LEGE 05292 were previously isolated and their ability to condition microcommunities by allelopathic effect was described. These interesting bioactive properties are, however, still underexplored as their biotechnological applications may be vast. This study aims to investigate the antifouling potential of portoamides, given that a challenge in the search for new environmentally friendly antifouling products is to find non-toxic natural alternatives with the ability to prevent colonization of different biofouling species, from bacteria to macroinvertebrates. A multi-bioassay approach was applied to assess portoamides antifouling properties, marine ecotoxicity and molecular mode of action. Results showed high effectiveness in the prevention of mussel larvae settlement (EC50 = 3.16 µM), and also bioactivity towards growth and biofilm disruption of marine biofouling bacterial strains, while not showing toxicity towards both target and non-target species. Antifouling molecular targets in mussel larvae include energy metabolism modifications (failure in proton-transporting ATPases activity), structural alterations of the gills and protein and gene regulatory mechanisms. Overall, portoamides reveal a broad-spectrum bioactivity towards diverse biofouling species, including a non-toxic and reversible effect towards mussel larvae, showing potential to be incorporated as an active ingredient in antifouling coatings.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/130450
url https://hdl.handle.net/10216/130450
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv ISSN 1660-3397
https://doi.org/10.3390/md17020111
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
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