High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus

• Autor(a) principal: da Cunha, Aureliano Claret
• Data de Publicação: 2019
• Outros Autores: Gomes, Lorena Soares, Godoy-Santos, Fernanda, Faria-Oliveira, Fábio, Teixeira, Janaína Aparecida, Sampaio, Geraldo Magela Santos, Trópia, Maria José Magalhães, Castro, Ieso Miranda, Lucas, Cândida, Brandão, Rogelio Lopes
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/73415
• topic: Aerobiosis; Alcoholic Beverages; Biofuels; Biomass; Bioreactors; Brazil; Candida; Chromatography, High Pressure Liquid; Cyanides; Ethanol; Fermentation; Food Technology; Glucose; Glycerol; Hydrogen-Ion Concentration; Industrial Microbiology; Kinetics; Protons; Saccharomyces cerevisiae; Saccharomycetales; Wickerhamomyces anomalus; Glycerol metabolism; Cachaca; Biotechnological applications; Glycerol transport; STL1; Ciências Naturais::Ciências Biológicas; Science & Technology

Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue.