High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/73415 |
Resumo: | Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue. |
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High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalusAerobiosisAlcoholic BeveragesBiofuelsBiomassBioreactorsBrazilCandidaChromatography, High Pressure LiquidCyanidesEthanolFermentationFood TechnologyGlucoseGlycerolHydrogen-Ion ConcentrationIndustrial MicrobiologyKineticsProtonsSaccharomyces cerevisiaeSaccharomycetalesWickerhamomyces anomalusGlycerol metabolismCachacaBiotechnological applicationsGlycerol transportSTL1Ciências Naturais::Ciências BiológicasScience & TechnologyWickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue.This work was supported by grants from Fundacao de Capacitacao de Pessoal de Nivel Superior from the Ministry of Education-CAPES/Brazil (PNPD 2755/2011; PCF-PVE 021/2012), from FEDER through POFC-COMPETE and by FCT through strategic funding (UID/BIA/04050/2013), from Universidade Federal de Ouro Preto, and a research fellowship from Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq (Brazil) Process 304815/2012 (research grant) and Process 305135/2015-5 (research fellowship to R.L.B.). C.L. is supported by the strategic program UID/BIA/04050/2013 [POCI-01-0145-FEDER-007569] funded by national funds through the FCT I. P. and by the ERDF through the COMPETE2020-Programa Operacional Competitividade e (POCI). The AUXPE-PVES 1801/2012 (Process 23038.015294/2016-18) from Brazilian Government supported a grant of Visiting Professor to C.L. and a research fellowships to A.C.C. and to F.F.O.SpringerUniversidade do Minhoda Cunha, Aureliano ClaretGomes, Lorena SoaresGodoy-Santos, FernandaFaria-Oliveira, FábioTeixeira, Janaína AparecidaSampaio, Geraldo Magela SantosTrópia, Maria José MagalhãesCastro, Ieso MirandaLucas, CândidaBrandão, Rogelio Lopes2019-052019-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/73415engAureliano Claret da Cunha, Lorena Soares Gomes, Fernanda Godoy-Santos, Fábio Faria-Oliveira, Janaína Aparecida Teixeira, Geraldo Magela Santos Sampaio, Maria José Magalhães Trópia, Ieso Miranda Castro, Cândida Lucas, Rogelio Lopes Brandão, High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus, Journal of Industrial Microbiology and Biotechnology, Volume 46, Issue 5, 1 May 2019, Pages 709–723, https://doi.org/10.1007/s10295-018-02119-51367-543510.1007/s10295-018-02119-530680472https://academic.oup.com/jimb/article/46/5/709/5997160info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:24:51Zoai:repositorium.sdum.uminho.pt:1822/73415Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:18:58.144773Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus |
title |
High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus |
spellingShingle |
High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus da Cunha, Aureliano Claret Aerobiosis Alcoholic Beverages Biofuels Biomass Bioreactors Brazil Candida Chromatography, High Pressure Liquid Cyanides Ethanol Fermentation Food Technology Glucose Glycerol Hydrogen-Ion Concentration Industrial Microbiology Kinetics Protons Saccharomyces cerevisiae Saccharomycetales Wickerhamomyces anomalus Glycerol metabolism Cachaca Biotechnological applications Glycerol transport STL1 Ciências Naturais::Ciências Biológicas Science & Technology |
title_short |
High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus |
title_full |
High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus |
title_fullStr |
High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus |
title_full_unstemmed |
High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus |
title_sort |
High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus |
author |
da Cunha, Aureliano Claret |
author_facet |
da Cunha, Aureliano Claret Gomes, Lorena Soares Godoy-Santos, Fernanda Faria-Oliveira, Fábio Teixeira, Janaína Aparecida Sampaio, Geraldo Magela Santos Trópia, Maria José Magalhães Castro, Ieso Miranda Lucas, Cândida Brandão, Rogelio Lopes |
author_role |
author |
author2 |
Gomes, Lorena Soares Godoy-Santos, Fernanda Faria-Oliveira, Fábio Teixeira, Janaína Aparecida Sampaio, Geraldo Magela Santos Trópia, Maria José Magalhães Castro, Ieso Miranda Lucas, Cândida Brandão, Rogelio Lopes |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
da Cunha, Aureliano Claret Gomes, Lorena Soares Godoy-Santos, Fernanda Faria-Oliveira, Fábio Teixeira, Janaína Aparecida Sampaio, Geraldo Magela Santos Trópia, Maria José Magalhães Castro, Ieso Miranda Lucas, Cândida Brandão, Rogelio Lopes |
dc.subject.por.fl_str_mv |
Aerobiosis Alcoholic Beverages Biofuels Biomass Bioreactors Brazil Candida Chromatography, High Pressure Liquid Cyanides Ethanol Fermentation Food Technology Glucose Glycerol Hydrogen-Ion Concentration Industrial Microbiology Kinetics Protons Saccharomyces cerevisiae Saccharomycetales Wickerhamomyces anomalus Glycerol metabolism Cachaca Biotechnological applications Glycerol transport STL1 Ciências Naturais::Ciências Biológicas Science & Technology |
topic |
Aerobiosis Alcoholic Beverages Biofuels Biomass Bioreactors Brazil Candida Chromatography, High Pressure Liquid Cyanides Ethanol Fermentation Food Technology Glucose Glycerol Hydrogen-Ion Concentration Industrial Microbiology Kinetics Protons Saccharomyces cerevisiae Saccharomycetales Wickerhamomyces anomalus Glycerol metabolism Cachaca Biotechnological applications Glycerol transport STL1 Ciências Naturais::Ciências Biológicas Science & Technology |
description |
Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-05 2019-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/73415 |
url |
http://hdl.handle.net/1822/73415 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aureliano Claret da Cunha, Lorena Soares Gomes, Fernanda Godoy-Santos, Fábio Faria-Oliveira, Janaína Aparecida Teixeira, Geraldo Magela Santos Sampaio, Maria José Magalhães Trópia, Ieso Miranda Castro, Cândida Lucas, Rogelio Lopes Brandão, High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus, Journal of Industrial Microbiology and Biotechnology, Volume 46, Issue 5, 1 May 2019, Pages 709–723, https://doi.org/10.1007/s10295-018-02119-5 1367-5435 10.1007/s10295-018-02119-5 30680472 https://academic.oup.com/jimb/article/46/5/709/5997160 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132646036471808 |