Deacetylation and Desuccinylation of the Fucose-Rich Polysaccharide Fucopol

Detalhes bibliográficos
Autor(a) principal: Baptista, Sílvia
Data de Publicação: 2022
Outros Autores: Araújo, Diana, Concórdio-Reis, Patrícia, Marques, Ana C., Fortunato, Elvira, Alves, Vítor D., Freitas, Filomena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/150576
Resumo: LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB Publisher Copyright: © 2022 by the authors.
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spelling Deacetylation and Desuccinylation of the Fucose-Rich Polysaccharide FucopolImpact on Biopolymer Physical and Chemical Propertiesalkaline treatmentdeacetylationdesuccinylationemulsionfilm formingFucoPolpolysacchariderheologyAnalytical ChemistryChemistry (miscellaneous)Molecular MedicinePharmaceutical ScienceDrug DiscoveryPhysical and Theoretical ChemistryOrganic ChemistryLA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB Publisher Copyright: © 2022 by the authors.FucoPol is an acylated polysaccharide with demonstrated valuable functional properties that include a shear thinning fluid behaviour, a film-forming capacity, and an emulsion forming and stabilizing capacity. In this study, the different conditions (concentration, temperature, and time) for alkaline treatment were investigated to deacylate FucoPol. Complete deacetylation and desuccinylation was achieved with 0.02 M NaOH, at 60 °C for 15 min, with no significant impact on the biopolymer’s sugar composition, pyruvate content, and molecular mass distribution. FucoPol depyruvylation by acid hydrolysis was attempted, but it resulted in a very low polymer recovery. The effect of the ionic strength, pH, and temperature on the deacetylated/desuccinylated polysaccharide, d-FucoPol, was evaluated, as well as its emulsion and film-forming capacity. d-FucoPol aqueous solutions maintained the shear thinning behaviour characteristic of FucoPol, but the apparent viscosity decreased significantly. Moreover, contrary to FucoPol, whose solutions were not affected by the media’s ionic strength, the d-FucoPol solutions had a significantly higher apparent viscosity for a higher ionic strength. On the other hand, the d-FucoPol solutions were not affected by the pH in the range of 3.6–11.5, while FucoPol had a decreased viscosity for acidic pH values and for a pH above 10.5. Although d-FucoPol displayed an emulsification activity for olive oil similar to that of FucoPol (98 ± 0%) for an oil-to-water ratio of 2:3, the emulsions were less viscous. The d-FucoPol films were flexible, with a higher Young′s modulus (798 ± 152 MPa), a stress at the break (22.5 ± 2.5 MPa), and an elongation at the break (9.3 ± 0.7%) than FucoPol (458 ± 32 MPa, 15.5 ± 0.3 MPa and 8.1 ± 1.0%, respectively). Given these findings, d-FucoPol arises as a promising novel biopolymer, with distinctive properties that may render it useful for utilization as a suspending or emulsifier agent, and as a barrier in coatings and packaging films.DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DCM - Departamento de Ciência dos MateriaisUNINOVA-Instituto de Desenvolvimento de Novas TecnologiasRUNBaptista, SílviaAraújo, DianaConcórdio-Reis, PatríciaMarques, Ana C.Fortunato, ElviraAlves, Vítor D.Freitas, Filomena2023-03-14T22:45:03Z2022-10-232022-10-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article18application/pdfhttp://hdl.handle.net/10362/150576eng1420-3049PURE: 55729464https://doi.org/10.3390/molecules27217165info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:09:58Zoai:run.unl.pt:10362/150576Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:09:58Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Deacetylation and Desuccinylation of the Fucose-Rich Polysaccharide Fucopol
Impact on Biopolymer Physical and Chemical Properties
title Deacetylation and Desuccinylation of the Fucose-Rich Polysaccharide Fucopol
spellingShingle Deacetylation and Desuccinylation of the Fucose-Rich Polysaccharide Fucopol
Baptista, Sílvia
alkaline treatment
deacetylation
desuccinylation
emulsion
film forming
FucoPol
polysaccharide
rheology
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
title_short Deacetylation and Desuccinylation of the Fucose-Rich Polysaccharide Fucopol
title_full Deacetylation and Desuccinylation of the Fucose-Rich Polysaccharide Fucopol
title_fullStr Deacetylation and Desuccinylation of the Fucose-Rich Polysaccharide Fucopol
title_full_unstemmed Deacetylation and Desuccinylation of the Fucose-Rich Polysaccharide Fucopol
title_sort Deacetylation and Desuccinylation of the Fucose-Rich Polysaccharide Fucopol
author Baptista, Sílvia
author_facet Baptista, Sílvia
Araújo, Diana
Concórdio-Reis, Patrícia
Marques, Ana C.
Fortunato, Elvira
Alves, Vítor D.
Freitas, Filomena
author_role author
author2 Araújo, Diana
Concórdio-Reis, Patrícia
Marques, Ana C.
Fortunato, Elvira
Alves, Vítor D.
Freitas, Filomena
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
DCM - Departamento de Ciência dos Materiais
UNINOVA-Instituto de Desenvolvimento de Novas Tecnologias
RUN
dc.contributor.author.fl_str_mv Baptista, Sílvia
Araújo, Diana
Concórdio-Reis, Patrícia
Marques, Ana C.
Fortunato, Elvira
Alves, Vítor D.
Freitas, Filomena
dc.subject.por.fl_str_mv alkaline treatment
deacetylation
desuccinylation
emulsion
film forming
FucoPol
polysaccharide
rheology
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
topic alkaline treatment
deacetylation
desuccinylation
emulsion
film forming
FucoPol
polysaccharide
rheology
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
description LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB Publisher Copyright: © 2022 by the authors.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-23
2022-10-23T00:00:00Z
2023-03-14T22:45:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/150576
url http://hdl.handle.net/10362/150576
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1420-3049
PURE: 55729464
https://doi.org/10.3390/molecules27217165
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 18
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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