Assessing the gene silencing potential of AuNP-based approaches on conventional 2D cell culture versus 3D tumor spheroid

Detalhes bibliográficos
Autor(a) principal: Oliveira, Beatriz B.
Data de Publicação: 2024
Outros Autores: Fernandes, Alexandra R., Baptista, Pedro Viana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/166791
Resumo: Publisher Copyright: Copyright © 2024 Oliveira, Fernandes and Baptista.
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spelling Assessing the gene silencing potential of AuNP-based approaches on conventional 2D cell culture versus 3D tumor spheroid3D spheroidsc-MYCcancer modelsgene silencinggold nanoparticlesBiotechnologyBioengineeringHistologyBiomedical EngineeringSDG 3 - Good Health and Well-beingPublisher Copyright: Copyright © 2024 Oliveira, Fernandes and Baptista.Three-dimensional (3D) cell culture using tumor spheroids provides a crucial platform for replicating tissue microenvironments. However, effective gene modulation via nanoparticle-based transfection remains a challenge, often facing delivery hurdles. Gold nanoparticles (AuNPs) with their tailored synthesis and biocompatibility, have shown promising results in two-dimensional (2D) cultures, nevertheless, they still require a comprehensive evaluation before they can reach its full potential on 3D models. While 2D cultures offer simplicity and affordability, they lack physiological fidelity. In contrast, 3D spheroids better capture in vivo conditions, enabling the study of cell interactions and nutrient distribution. These models are essential for investigating cancer behavior, drug responses, and developmental processes. Nevertheless, transitioning from 2D to 3D models demands an understanding of altered internalization mechanisms and microenvironmental influences. This study assessed ASO-AuNP conjugates for silencing the c-MYC oncogene in 2D cultures and 3D tumor spheroids, revealing distinctions in gene silencing efficiency and highlighting the microenvironment’s impact on AuNP-mediated gene modulation. Herein, we demonstrate that increasing the number of AuNPs per cell by 2.6 times, when transitioning from a 2D cell model to a 3D spheroid, allows to attain similar silencing efficiencies. Such insights advance the development of targeted gene therapies within intricate tissue-like contexts.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNOliveira, Beatriz B.Fernandes, Alexandra R.Baptista, Pedro Viana2024-04-30T02:45:25Z20242024-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://hdl.handle.net/10362/166791eng2296-4185PURE: 89477968https://doi.org/10.3389/fbioe.2024.1320729info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-09T01:38:32Zoai:run.unl.pt:10362/166791Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-09T01:38:32Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Assessing the gene silencing potential of AuNP-based approaches on conventional 2D cell culture versus 3D tumor spheroid
title Assessing the gene silencing potential of AuNP-based approaches on conventional 2D cell culture versus 3D tumor spheroid
spellingShingle Assessing the gene silencing potential of AuNP-based approaches on conventional 2D cell culture versus 3D tumor spheroid
Oliveira, Beatriz B.
3D spheroids
c-MYC
cancer models
gene silencing
gold nanoparticles
Biotechnology
Bioengineering
Histology
Biomedical Engineering
SDG 3 - Good Health and Well-being
title_short Assessing the gene silencing potential of AuNP-based approaches on conventional 2D cell culture versus 3D tumor spheroid
title_full Assessing the gene silencing potential of AuNP-based approaches on conventional 2D cell culture versus 3D tumor spheroid
title_fullStr Assessing the gene silencing potential of AuNP-based approaches on conventional 2D cell culture versus 3D tumor spheroid
title_full_unstemmed Assessing the gene silencing potential of AuNP-based approaches on conventional 2D cell culture versus 3D tumor spheroid
title_sort Assessing the gene silencing potential of AuNP-based approaches on conventional 2D cell culture versus 3D tumor spheroid
author Oliveira, Beatriz B.
author_facet Oliveira, Beatriz B.
Fernandes, Alexandra R.
Baptista, Pedro Viana
author_role author
author2 Fernandes, Alexandra R.
Baptista, Pedro Viana
author2_role author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Oliveira, Beatriz B.
Fernandes, Alexandra R.
Baptista, Pedro Viana
dc.subject.por.fl_str_mv 3D spheroids
c-MYC
cancer models
gene silencing
gold nanoparticles
Biotechnology
Bioengineering
Histology
Biomedical Engineering
SDG 3 - Good Health and Well-being
topic 3D spheroids
c-MYC
cancer models
gene silencing
gold nanoparticles
Biotechnology
Bioengineering
Histology
Biomedical Engineering
SDG 3 - Good Health and Well-being
description Publisher Copyright: Copyright © 2024 Oliveira, Fernandes and Baptista.
publishDate 2024
dc.date.none.fl_str_mv 2024-04-30T02:45:25Z
2024
2024-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/166791
url http://hdl.handle.net/10362/166791
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2296-4185
PURE: 89477968
https://doi.org/10.3389/fbioe.2024.1320729
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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