Diagnostic Assays for Crimean-Congo hemorrhagic fever

• Autor(a) principal: Vanhomwegen, J.
• Data de Publicação: 2012
• Outros Autores: Alves, M.J., Zupanc, T.A., Bino, S., Chinikar, S., Karlberg, H., Korukluoglu, G., Korva, M., Mardani, M., Mirazimi, A., Mousavi, M., Papa, A., Saksida, A., Sharifi-Mood, B., Sidira, P., Tsergouli, K., Wolfel, R., Zeller, H., Dubois, P.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.18/1176
• topic: Infecções Sistémicas e Zoonoses; Crimean-Congo; Hemorrhagic Fever; Diagnostic Assays

Crimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-borne disease with case-fatality rates as high as 50%. We describe a collaborative evaluation of the characteristics, performance, and on-site applicability of serologic and molecular assays for diagnosis of CCHF. We evaluated ELISA, immunofluorescence, quantitative reverse transcription PCR, and low-density macroarray assays for detection of CCHF virus using precharacterized archived patient serum samples. Compared with results of local, in-house methods, test sensitivities were 87.8%-93.9% for IgM serology, 80.4%-86.1% for IgG serology, and 79.6%-83.3% for genome detection. Specificity was excellent for all assays; molecular test results were influenced by patient country of origin. Our findings demonstrate that well-characterized, reliable tools are available for CCHF diagnosis and surveillance. The on-site use of such assays by health laboratories would greatly diminish the time, costs, and risks posed by the handling, packaging, and shipping of highly infectious biologic material.