Diagnostic Assays for Crimean-Congo hemorrhagic fever
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/1176 |
Resumo: | Crimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-borne disease with case-fatality rates as high as 50%. We describe a collaborative evaluation of the characteristics, performance, and on-site applicability of serologic and molecular assays for diagnosis of CCHF. We evaluated ELISA, immunofluorescence, quantitative reverse transcription PCR, and low-density macroarray assays for detection of CCHF virus using precharacterized archived patient serum samples. Compared with results of local, in-house methods, test sensitivities were 87.8%-93.9% for IgM serology, 80.4%-86.1% for IgG serology, and 79.6%-83.3% for genome detection. Specificity was excellent for all assays; molecular test results were influenced by patient country of origin. Our findings demonstrate that well-characterized, reliable tools are available for CCHF diagnosis and surveillance. The on-site use of such assays by health laboratories would greatly diminish the time, costs, and risks posed by the handling, packaging, and shipping of highly infectious biologic material. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Diagnostic Assays for Crimean-Congo hemorrhagic feverInfecções Sistémicas e ZoonosesCrimean-CongoHemorrhagic FeverDiagnostic AssaysCrimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-borne disease with case-fatality rates as high as 50%. We describe a collaborative evaluation of the characteristics, performance, and on-site applicability of serologic and molecular assays for diagnosis of CCHF. We evaluated ELISA, immunofluorescence, quantitative reverse transcription PCR, and low-density macroarray assays for detection of CCHF virus using precharacterized archived patient serum samples. Compared with results of local, in-house methods, test sensitivities were 87.8%-93.9% for IgM serology, 80.4%-86.1% for IgG serology, and 79.6%-83.3% for genome detection. Specificity was excellent for all assays; molecular test results were influenced by patient country of origin. Our findings demonstrate that well-characterized, reliable tools are available for CCHF diagnosis and surveillance. The on-site use of such assays by health laboratories would greatly diminish the time, costs, and risks posed by the handling, packaging, and shipping of highly infectious biologic material.CDC-National Center for Infectious DiseasesRepositório Científico do Instituto Nacional de SaúdeVanhomwegen, J.Alves, M.J.Zupanc, T.A.Bino, S.Chinikar, S.Karlberg, H.Korukluoglu, G.Korva, M.Mardani, M.Mirazimi, A.Mousavi, M.Papa, A.Saksida, A.Sharifi-Mood, B.Sidira, P.Tsergouli, K.Wolfel, R.Zeller, H.Dubois, P.2013-01-28T13:49:48Z2012-122012-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/1176engEmerg Infect Dis. 2012 Dec;18(12):1958-651080-6040doi: 10.3201/eid1812.120710info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:38:35Zoai:repositorio.insa.pt:10400.18/1176Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:36:19.099844Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Diagnostic Assays for Crimean-Congo hemorrhagic fever |
title |
Diagnostic Assays for Crimean-Congo hemorrhagic fever |
spellingShingle |
Diagnostic Assays for Crimean-Congo hemorrhagic fever Vanhomwegen, J. Infecções Sistémicas e Zoonoses Crimean-Congo Hemorrhagic Fever Diagnostic Assays |
title_short |
Diagnostic Assays for Crimean-Congo hemorrhagic fever |
title_full |
Diagnostic Assays for Crimean-Congo hemorrhagic fever |
title_fullStr |
Diagnostic Assays for Crimean-Congo hemorrhagic fever |
title_full_unstemmed |
Diagnostic Assays for Crimean-Congo hemorrhagic fever |
title_sort |
Diagnostic Assays for Crimean-Congo hemorrhagic fever |
author |
Vanhomwegen, J. |
author_facet |
Vanhomwegen, J. Alves, M.J. Zupanc, T.A. Bino, S. Chinikar, S. Karlberg, H. Korukluoglu, G. Korva, M. Mardani, M. Mirazimi, A. Mousavi, M. Papa, A. Saksida, A. Sharifi-Mood, B. Sidira, P. Tsergouli, K. Wolfel, R. Zeller, H. Dubois, P. |
author_role |
author |
author2 |
Alves, M.J. Zupanc, T.A. Bino, S. Chinikar, S. Karlberg, H. Korukluoglu, G. Korva, M. Mardani, M. Mirazimi, A. Mousavi, M. Papa, A. Saksida, A. Sharifi-Mood, B. Sidira, P. Tsergouli, K. Wolfel, R. Zeller, H. Dubois, P. |
author2_role |
author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Vanhomwegen, J. Alves, M.J. Zupanc, T.A. Bino, S. Chinikar, S. Karlberg, H. Korukluoglu, G. Korva, M. Mardani, M. Mirazimi, A. Mousavi, M. Papa, A. Saksida, A. Sharifi-Mood, B. Sidira, P. Tsergouli, K. Wolfel, R. Zeller, H. Dubois, P. |
dc.subject.por.fl_str_mv |
Infecções Sistémicas e Zoonoses Crimean-Congo Hemorrhagic Fever Diagnostic Assays |
topic |
Infecções Sistémicas e Zoonoses Crimean-Congo Hemorrhagic Fever Diagnostic Assays |
description |
Crimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-borne disease with case-fatality rates as high as 50%. We describe a collaborative evaluation of the characteristics, performance, and on-site applicability of serologic and molecular assays for diagnosis of CCHF. We evaluated ELISA, immunofluorescence, quantitative reverse transcription PCR, and low-density macroarray assays for detection of CCHF virus using precharacterized archived patient serum samples. Compared with results of local, in-house methods, test sensitivities were 87.8%-93.9% for IgM serology, 80.4%-86.1% for IgG serology, and 79.6%-83.3% for genome detection. Specificity was excellent for all assays; molecular test results were influenced by patient country of origin. Our findings demonstrate that well-characterized, reliable tools are available for CCHF diagnosis and surveillance. The on-site use of such assays by health laboratories would greatly diminish the time, costs, and risks posed by the handling, packaging, and shipping of highly infectious biologic material. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12 2012-12-01T00:00:00Z 2013-01-28T13:49:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/1176 |
url |
http://hdl.handle.net/10400.18/1176 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Emerg Infect Dis. 2012 Dec;18(12):1958-65 1080-6040 doi: 10.3201/eid1812.120710 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
CDC-National Center for Infectious Diseases |
publisher.none.fl_str_mv |
CDC-National Center for Infectious Diseases |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132094492835840 |