Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery

Detalhes bibliográficos
Autor(a) principal: Santos, J. A. L.
Data de Publicação: 1996
Outros Autores: Belo, Isabel, Mota, M., Cabral, J. M. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/33607
Resumo: The influence of sonication power, suspension volume and cell concentration on the kinetics of cytochrome b5 and intracellular protein release by sonication of Escherichia coli TB1 cells was studied. The influence of freezing and thawing of the cell suspension was also evaluated. Freezing and thawing increased the recovery yield of cytochrome b5. The sonication efficiency increased with the increase of sonication power and with the decrease of the suspension volume and cell concentration.
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spelling Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recoveryCell disruptionFreeze/thawingSonicationEscherichia coliCytochrome b5Science & TechnologyThe influence of sonication power, suspension volume and cell concentration on the kinetics of cytochrome b5 and intracellular protein release by sonication of Escherichia coli TB1 cells was studied. The influence of freezing and thawing of the cell suspension was also evaluated. Freezing and thawing increased the recovery yield of cytochrome b5. The sonication efficiency increased with the increase of sonication power and with the decrease of the suspension volume and cell concentration.KluwerUniversidade do MinhoSantos, J. A. L.Belo, IsabelMota, M.Cabral, J. M. S.19961996-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/33607engSantos, J. A. L.; Belo, Isabel; Mota, M.; Cabral, J. M. S., Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery. Bioseparation, 6, 81-89, 19960923-179X1573-82728818263info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:38:54Zoai:repositorium.sdum.uminho.pt:1822/33607Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:35:26.088652Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery
title Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery
spellingShingle Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery
Santos, J. A. L.
Cell disruption
Freeze/thawing
Sonication
Escherichia coli
Cytochrome b5
Science & Technology
title_short Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery
title_full Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery
title_fullStr Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery
title_full_unstemmed Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery
title_sort Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery
author Santos, J. A. L.
author_facet Santos, J. A. L.
Belo, Isabel
Mota, M.
Cabral, J. M. S.
author_role author
author2 Belo, Isabel
Mota, M.
Cabral, J. M. S.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Santos, J. A. L.
Belo, Isabel
Mota, M.
Cabral, J. M. S.
dc.subject.por.fl_str_mv Cell disruption
Freeze/thawing
Sonication
Escherichia coli
Cytochrome b5
Science & Technology
topic Cell disruption
Freeze/thawing
Sonication
Escherichia coli
Cytochrome b5
Science & Technology
description The influence of sonication power, suspension volume and cell concentration on the kinetics of cytochrome b5 and intracellular protein release by sonication of Escherichia coli TB1 cells was studied. The influence of freezing and thawing of the cell suspension was also evaluated. Freezing and thawing increased the recovery yield of cytochrome b5. The sonication efficiency increased with the increase of sonication power and with the decrease of the suspension volume and cell concentration.
publishDate 1996
dc.date.none.fl_str_mv 1996
1996-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/33607
url http://hdl.handle.net/1822/33607
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Santos, J. A. L.; Belo, Isabel; Mota, M.; Cabral, J. M. S., Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery. Bioseparation, 6, 81-89, 1996
0923-179X
1573-8272
8818263
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Kluwer
publisher.none.fl_str_mv Kluwer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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