Yeast plasma membrane Fungal Oligopeptide Transporters display distinct substrate preferences despite their high sequence identity
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/76480 |
Resumo: | Fungal Oligopeptide Transporters (Fot) Fot1, Fot2 and Fot3 have been found in <i>Saccharomyces cerevisiae</i> wine strains, but not in strains from other environments. In the <i>S. cerevisiae</i> wine strain EC1118, Fot1 and Fot2 are responsible for a broader range of oligopeptide utilization in comparison with strains not containing any Fot. This leads to better fermentation efficiency and an increased production of desirable organoleptic compounds in wine. Despite the benefits associated with Fot activity in <i>S. cerevisiae</i> within the wine environment, little is known about this family of transporters in yeast. The presence of Fot1, Fot2 and Fot3 in <i>S. cerevisiae</i> wine strains is due to horizontal gene transfer from the yeast <i>Torulaspora microellipsoides</i>, which harbors Fot2Tm, FotX and FotY proteins. Sequence analyses revealed that Fot family members have a high sequence identity in these yeast species. In this work, we aimed to further characterize the different Fot family members in terms of subcellular localization, gene expression in enological fermentation and substrate specificity. Using CRISPR/Cas9, we constructed <i>S. cerevisiae</i> wine strains containing each different Fot as the sole oligopeptide transporter to analyze their oligopeptide preferences by phenotype microarrays. The results of oligopeptide consumption show that Fot counterparts have different di-/tripeptide specificities, suggesting that punctual sequence divergence between <i>FOT</i> genes can be crucial for substrate recognition, binding and transport activity. <i>FOT</i> gene expression levels in different <i>S. cerevisiae</i> wine strains during enological fermentation, together with predicted binding motifs for transcriptional regulators in nitrogen metabolism, indicate that these transporters may be under the control of the Nitrogen Catabolite Repression (NCR) system. Finally, we demonstrated that Fot1 is located in the yeast plasma membrane. This work contributes to a better understanding of this family of oligopeptide transporters, which have demonstrated a key role in the utilization of oligopeptides by <i>S. cerevisiae</i> in enological fermentation. |
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Yeast plasma membrane Fungal Oligopeptide Transporters display distinct substrate preferences despite their high sequence identityFungal Oligopeptide Transporters (Fot)Oligopeptide transportSaccharomyces cerevisiaePhenotype microarraysGFP labelingScience & TechnologyFungal Oligopeptide Transporters (Fot) Fot1, Fot2 and Fot3 have been found in <i>Saccharomyces cerevisiae</i> wine strains, but not in strains from other environments. In the <i>S. cerevisiae</i> wine strain EC1118, Fot1 and Fot2 are responsible for a broader range of oligopeptide utilization in comparison with strains not containing any Fot. This leads to better fermentation efficiency and an increased production of desirable organoleptic compounds in wine. Despite the benefits associated with Fot activity in <i>S. cerevisiae</i> within the wine environment, little is known about this family of transporters in yeast. The presence of Fot1, Fot2 and Fot3 in <i>S. cerevisiae</i> wine strains is due to horizontal gene transfer from the yeast <i>Torulaspora microellipsoides</i>, which harbors Fot2Tm, FotX and FotY proteins. Sequence analyses revealed that Fot family members have a high sequence identity in these yeast species. In this work, we aimed to further characterize the different Fot family members in terms of subcellular localization, gene expression in enological fermentation and substrate specificity. Using CRISPR/Cas9, we constructed <i>S. cerevisiae</i> wine strains containing each different Fot as the sole oligopeptide transporter to analyze their oligopeptide preferences by phenotype microarrays. The results of oligopeptide consumption show that Fot counterparts have different di-/tripeptide specificities, suggesting that punctual sequence divergence between <i>FOT</i> genes can be crucial for substrate recognition, binding and transport activity. <i>FOT</i> gene expression levels in different <i>S. cerevisiae</i> wine strains during enological fermentation, together with predicted binding motifs for transcriptional regulators in nitrogen metabolism, indicate that these transporters may be under the control of the Nitrogen Catabolite Repression (NCR) system. Finally, we demonstrated that Fot1 is located in the yeast plasma membrane. This work contributes to a better understanding of this family of oligopeptide transporters, which have demonstrated a key role in the utilization of oligopeptides by <i>S. cerevisiae</i> in enological fermentation.This project has received funding from the European Union’s Horizon 2020 Research and Innovation Program under the Marie Skłodowska-Curie Grant Agreement No. 764927Multidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoBecerra-Rodríguez, CarmenTaghouti, GéraldinePortier, PerrineDequin, SylvieCasal, MargaridaPaiva, SandraGaleote, Virginie2021-11-122021-11-12T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/76480engBecerra-Rodríguez, C.; Taghouti, G.; Portier, P.; Dequin, S.; Casal, M.; Paiva, S.; Galeote, V. Yeast Plasma Membrane Fungal Oligopeptide Transporters Display Distinct Substrate Preferences despite Their High Sequence Identity. J. Fungi 2021, 7, 963. https://doi.org/10.3390/jof71109632309-608X10.3390/jof7110963963https://www.mdpi.com/2309-608X/7/11/963info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:55:34Zoai:repositorium.sdum.uminho.pt:1822/76480Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:45:06.686746Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Yeast plasma membrane Fungal Oligopeptide Transporters display distinct substrate preferences despite their high sequence identity |
title |
Yeast plasma membrane Fungal Oligopeptide Transporters display distinct substrate preferences despite their high sequence identity |
spellingShingle |
Yeast plasma membrane Fungal Oligopeptide Transporters display distinct substrate preferences despite their high sequence identity Becerra-Rodríguez, Carmen Fungal Oligopeptide Transporters (Fot) Oligopeptide transport Saccharomyces cerevisiae Phenotype microarrays GFP labeling Science & Technology |
title_short |
Yeast plasma membrane Fungal Oligopeptide Transporters display distinct substrate preferences despite their high sequence identity |
title_full |
Yeast plasma membrane Fungal Oligopeptide Transporters display distinct substrate preferences despite their high sequence identity |
title_fullStr |
Yeast plasma membrane Fungal Oligopeptide Transporters display distinct substrate preferences despite their high sequence identity |
title_full_unstemmed |
Yeast plasma membrane Fungal Oligopeptide Transporters display distinct substrate preferences despite their high sequence identity |
title_sort |
Yeast plasma membrane Fungal Oligopeptide Transporters display distinct substrate preferences despite their high sequence identity |
author |
Becerra-Rodríguez, Carmen |
author_facet |
Becerra-Rodríguez, Carmen Taghouti, Géraldine Portier, Perrine Dequin, Sylvie Casal, Margarida Paiva, Sandra Galeote, Virginie |
author_role |
author |
author2 |
Taghouti, Géraldine Portier, Perrine Dequin, Sylvie Casal, Margarida Paiva, Sandra Galeote, Virginie |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Becerra-Rodríguez, Carmen Taghouti, Géraldine Portier, Perrine Dequin, Sylvie Casal, Margarida Paiva, Sandra Galeote, Virginie |
dc.subject.por.fl_str_mv |
Fungal Oligopeptide Transporters (Fot) Oligopeptide transport Saccharomyces cerevisiae Phenotype microarrays GFP labeling Science & Technology |
topic |
Fungal Oligopeptide Transporters (Fot) Oligopeptide transport Saccharomyces cerevisiae Phenotype microarrays GFP labeling Science & Technology |
description |
Fungal Oligopeptide Transporters (Fot) Fot1, Fot2 and Fot3 have been found in <i>Saccharomyces cerevisiae</i> wine strains, but not in strains from other environments. In the <i>S. cerevisiae</i> wine strain EC1118, Fot1 and Fot2 are responsible for a broader range of oligopeptide utilization in comparison with strains not containing any Fot. This leads to better fermentation efficiency and an increased production of desirable organoleptic compounds in wine. Despite the benefits associated with Fot activity in <i>S. cerevisiae</i> within the wine environment, little is known about this family of transporters in yeast. The presence of Fot1, Fot2 and Fot3 in <i>S. cerevisiae</i> wine strains is due to horizontal gene transfer from the yeast <i>Torulaspora microellipsoides</i>, which harbors Fot2Tm, FotX and FotY proteins. Sequence analyses revealed that Fot family members have a high sequence identity in these yeast species. In this work, we aimed to further characterize the different Fot family members in terms of subcellular localization, gene expression in enological fermentation and substrate specificity. Using CRISPR/Cas9, we constructed <i>S. cerevisiae</i> wine strains containing each different Fot as the sole oligopeptide transporter to analyze their oligopeptide preferences by phenotype microarrays. The results of oligopeptide consumption show that Fot counterparts have different di-/tripeptide specificities, suggesting that punctual sequence divergence between <i>FOT</i> genes can be crucial for substrate recognition, binding and transport activity. <i>FOT</i> gene expression levels in different <i>S. cerevisiae</i> wine strains during enological fermentation, together with predicted binding motifs for transcriptional regulators in nitrogen metabolism, indicate that these transporters may be under the control of the Nitrogen Catabolite Repression (NCR) system. Finally, we demonstrated that Fot1 is located in the yeast plasma membrane. This work contributes to a better understanding of this family of oligopeptide transporters, which have demonstrated a key role in the utilization of oligopeptides by <i>S. cerevisiae</i> in enological fermentation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-12 2021-11-12T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/76480 |
url |
http://hdl.handle.net/1822/76480 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Becerra-Rodríguez, C.; Taghouti, G.; Portier, P.; Dequin, S.; Casal, M.; Paiva, S.; Galeote, V. Yeast Plasma Membrane Fungal Oligopeptide Transporters Display Distinct Substrate Preferences despite Their High Sequence Identity. J. Fungi 2021, 7, 963. https://doi.org/10.3390/jof7110963 2309-608X 10.3390/jof7110963 963 https://www.mdpi.com/2309-608X/7/11/963 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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