Antemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis)
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/4648 |
Resumo: | The suitability of nested reverse transcription polymerase chain reaction (nRT-PCR) to detect betanodavirus in blood samples from naturally infected Senegalese sole (Solea senegalensis) was evaluated in comparison with other diagnostic methods. Results indicated that histologic examination of brain lesions could be regarded as the most consistent indicator of nodavirus infection in this species. The nRT-PCR showed low to moderate levels of detection; the best values were obtained in brain samples followed by blood samples. Inoculation of SSN-1 and SAF-1 cells with fish samples did not cause cytopathic effect, although virus was detected by reverse transcription polymerase chain reaction in approximately 25% of the SSN-1 inoculated wells. The efficiency of detection of the viral genome was dramatically increased by the use of nRTPCR, reaching 90.6% of positives in brain samples and 84.4% in blood samples. The sensitivity and the negative predictive value of nRT-PCR in blood samples were slightly lower than those obtained using brain samples. Nevertheless, it is suggested that the advantage of being able to perform diagnosis on live fish adequately counterbalances the slightly lower sensitivity of nRT-PCR on blood samples. This technique is proposed as a useful tool, not only for the selection of nodavirus-free breeders but also to check the fish status during ongrowing. |
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Antemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis)BetanodavirusBloodNested reverse transcription polymerase chain reactionThe suitability of nested reverse transcription polymerase chain reaction (nRT-PCR) to detect betanodavirus in blood samples from naturally infected Senegalese sole (Solea senegalensis) was evaluated in comparison with other diagnostic methods. Results indicated that histologic examination of brain lesions could be regarded as the most consistent indicator of nodavirus infection in this species. The nRT-PCR showed low to moderate levels of detection; the best values were obtained in brain samples followed by blood samples. Inoculation of SSN-1 and SAF-1 cells with fish samples did not cause cytopathic effect, although virus was detected by reverse transcription polymerase chain reaction in approximately 25% of the SSN-1 inoculated wells. The efficiency of detection of the viral genome was dramatically increased by the use of nRTPCR, reaching 90.6% of positives in brain samples and 84.4% in blood samples. The sensitivity and the negative predictive value of nRT-PCR in blood samples were slightly lower than those obtained using brain samples. Nevertheless, it is suggested that the advantage of being able to perform diagnosis on live fish adequately counterbalances the slightly lower sensitivity of nRT-PCR on blood samples. This technique is proposed as a useful tool, not only for the selection of nodavirus-free breeders but also to check the fish status during ongrowing.American Association of Veterinary Laboratory DiagnosticiansSapientiaOlveira, J. G.Soares, F.Engrola, SofiaDopazo, C. P.Bandín, I.2014-07-04T09:06:35Z20082014-07-03T11:26:07Z2008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/4648engOlveira, J.G.; Soares, F.; Engrola, S.; Dopazo, C.P.; Bandín, I. Antemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis), Journal of Veterinary Diagnostic Investigation, 20, 2, 215-219, 2008.1040-6387http://dx.doi.org/10.1177/104063870802000212info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:15:54Zoai:sapientia.ualg.pt:10400.1/4648Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:57:57.207979Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis) |
title |
Antemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis) |
spellingShingle |
Antemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis) Olveira, J. G. Betanodavirus Blood Nested reverse transcription polymerase chain reaction |
title_short |
Antemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis) |
title_full |
Antemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis) |
title_fullStr |
Antemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis) |
title_full_unstemmed |
Antemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis) |
title_sort |
Antemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis) |
author |
Olveira, J. G. |
author_facet |
Olveira, J. G. Soares, F. Engrola, Sofia Dopazo, C. P. Bandín, I. |
author_role |
author |
author2 |
Soares, F. Engrola, Sofia Dopazo, C. P. Bandín, I. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Olveira, J. G. Soares, F. Engrola, Sofia Dopazo, C. P. Bandín, I. |
dc.subject.por.fl_str_mv |
Betanodavirus Blood Nested reverse transcription polymerase chain reaction |
topic |
Betanodavirus Blood Nested reverse transcription polymerase chain reaction |
description |
The suitability of nested reverse transcription polymerase chain reaction (nRT-PCR) to detect betanodavirus in blood samples from naturally infected Senegalese sole (Solea senegalensis) was evaluated in comparison with other diagnostic methods. Results indicated that histologic examination of brain lesions could be regarded as the most consistent indicator of nodavirus infection in this species. The nRT-PCR showed low to moderate levels of detection; the best values were obtained in brain samples followed by blood samples. Inoculation of SSN-1 and SAF-1 cells with fish samples did not cause cytopathic effect, although virus was detected by reverse transcription polymerase chain reaction in approximately 25% of the SSN-1 inoculated wells. The efficiency of detection of the viral genome was dramatically increased by the use of nRTPCR, reaching 90.6% of positives in brain samples and 84.4% in blood samples. The sensitivity and the negative predictive value of nRT-PCR in blood samples were slightly lower than those obtained using brain samples. Nevertheless, it is suggested that the advantage of being able to perform diagnosis on live fish adequately counterbalances the slightly lower sensitivity of nRT-PCR on blood samples. This technique is proposed as a useful tool, not only for the selection of nodavirus-free breeders but also to check the fish status during ongrowing. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2008-01-01T00:00:00Z 2014-07-04T09:06:35Z 2014-07-03T11:26:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/4648 |
url |
http://hdl.handle.net/10400.1/4648 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Olveira, J.G.; Soares, F.; Engrola, S.; Dopazo, C.P.; Bandín, I. Antemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis), Journal of Veterinary Diagnostic Investigation, 20, 2, 215-219, 2008. 1040-6387 http://dx.doi.org/10.1177/104063870802000212 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Association of Veterinary Laboratory Diagnosticians |
publisher.none.fl_str_mv |
American Association of Veterinary Laboratory Diagnosticians |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133193546235904 |