Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection

• Autor(a) principal: Carnieli Junior,Pedro
• Data de Publicação: 2006
• Outros Autores: Ventura,Armando Moraes, Durigon,Edison Luiz
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822006000200005
• topic: Rabies; N Gene; Reverse transcription polymerase chain reaction; Probe; Hybridization

A digoxigenin-labeled probe was produced from the Pasteur virus strain for the detection of the rabies virus N gene. The probe hybridization was performed from amplified N gene obtained by reverse transcription polymerase chain reaction and the results by RT-PCR and hybridization showed 100% agreement. The hybridization, when carried out in products amplified by RT-PCR, increases the sensitivity of this technique even more and confers specificity to the diagnosis. The technique described in this work will be useful in rabies diagnosis laboratories, once the cost is compatible with traditional rabies diagnostic techniques.