Development of a novel methodology for the analysis of ergosterol in mushrooms
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/9503 |
Resumo: | Sterols are important molecules in the unsaponifiable fraction of several matrices. Ergosterol, which is an important vitamin D2 precursor, is clearly the main sterol in mushrooms. Herein, an analytical method for ergosterol determination in cultivated and wild mushrooms was developed using high-performance liquid chromatography coupled to ultraviolet detection. The chromatographic separation was achieved in an Inertsil 100A ODS-3 reversed-phase column using an isocratic elution with acetonitrile/methanol (70:30, v/v) at a flow rate of 1 mL/min. Different extraction methodologies were tested, using n-hexane, methanol/dichloromethane (75:25, v/v) or chloroform/methanol (20:10, v/v). The method was optimised using Fistulina hepatica and proved to be reproductive and accurate. Ergosterol was the most abundant sterol by a greater extent in all mushrooms. In general, the cultivated species showed higher contents, mainly Agaricus bisporus and Lentinus edodes. Among wild species, Boletus edulis was the mushroom with the highest content in ergosterol. Results were expressed in fat content, dry weight and fresh weight bases. The assessment of ergosterol amounts might be very useful due to the bioactive potential that has been attributed to this molecule and its derivatives. |
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Development of a novel methodology for the analysis of ergosterol in mushroomsErgosterolAnalysis optimizationHPLC-UVMushroomsSterols are important molecules in the unsaponifiable fraction of several matrices. Ergosterol, which is an important vitamin D2 precursor, is clearly the main sterol in mushrooms. Herein, an analytical method for ergosterol determination in cultivated and wild mushrooms was developed using high-performance liquid chromatography coupled to ultraviolet detection. The chromatographic separation was achieved in an Inertsil 100A ODS-3 reversed-phase column using an isocratic elution with acetonitrile/methanol (70:30, v/v) at a flow rate of 1 mL/min. Different extraction methodologies were tested, using n-hexane, methanol/dichloromethane (75:25, v/v) or chloroform/methanol (20:10, v/v). The method was optimised using Fistulina hepatica and proved to be reproductive and accurate. Ergosterol was the most abundant sterol by a greater extent in all mushrooms. In general, the cultivated species showed higher contents, mainly Agaricus bisporus and Lentinus edodes. Among wild species, Boletus edulis was the mushroom with the highest content in ergosterol. Results were expressed in fat content, dry weight and fresh weight bases. The assessment of ergosterol amounts might be very useful due to the bioactive potential that has been attributed to this molecule and its derivatives.SpringerBiblioteca Digital do IPBBarreira, João C.M.Oliveira, BeatrizFerreira, Isabel C.F.R.2014-05-13T11:10:14Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10198/9503engBarreira, João C.M.; Oliveira, M. Beatriz P.P.; Ferreira, Isabel C.F.R. (2014). Development of a novel methodology for the analysis of ergosterol in mushrooms. Food Analytical Methods. ISSN 1936-9751. 7:1, p. 217-2231936-975110.1007/s12161-013-9621-9info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:22:10Zoai:bibliotecadigital.ipb.pt:10198/9503Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:00:27.359484Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development of a novel methodology for the analysis of ergosterol in mushrooms |
title |
Development of a novel methodology for the analysis of ergosterol in mushrooms |
spellingShingle |
Development of a novel methodology for the analysis of ergosterol in mushrooms Barreira, João C.M. Ergosterol Analysis optimization HPLC-UV Mushrooms |
title_short |
Development of a novel methodology for the analysis of ergosterol in mushrooms |
title_full |
Development of a novel methodology for the analysis of ergosterol in mushrooms |
title_fullStr |
Development of a novel methodology for the analysis of ergosterol in mushrooms |
title_full_unstemmed |
Development of a novel methodology for the analysis of ergosterol in mushrooms |
title_sort |
Development of a novel methodology for the analysis of ergosterol in mushrooms |
author |
Barreira, João C.M. |
author_facet |
Barreira, João C.M. Oliveira, Beatriz Ferreira, Isabel C.F.R. |
author_role |
author |
author2 |
Oliveira, Beatriz Ferreira, Isabel C.F.R. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Barreira, João C.M. Oliveira, Beatriz Ferreira, Isabel C.F.R. |
dc.subject.por.fl_str_mv |
Ergosterol Analysis optimization HPLC-UV Mushrooms |
topic |
Ergosterol Analysis optimization HPLC-UV Mushrooms |
description |
Sterols are important molecules in the unsaponifiable fraction of several matrices. Ergosterol, which is an important vitamin D2 precursor, is clearly the main sterol in mushrooms. Herein, an analytical method for ergosterol determination in cultivated and wild mushrooms was developed using high-performance liquid chromatography coupled to ultraviolet detection. The chromatographic separation was achieved in an Inertsil 100A ODS-3 reversed-phase column using an isocratic elution with acetonitrile/methanol (70:30, v/v) at a flow rate of 1 mL/min. Different extraction methodologies were tested, using n-hexane, methanol/dichloromethane (75:25, v/v) or chloroform/methanol (20:10, v/v). The method was optimised using Fistulina hepatica and proved to be reproductive and accurate. Ergosterol was the most abundant sterol by a greater extent in all mushrooms. In general, the cultivated species showed higher contents, mainly Agaricus bisporus and Lentinus edodes. Among wild species, Boletus edulis was the mushroom with the highest content in ergosterol. Results were expressed in fat content, dry weight and fresh weight bases. The assessment of ergosterol amounts might be very useful due to the bioactive potential that has been attributed to this molecule and its derivatives. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-05-13T11:10:14Z 2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/9503 |
url |
http://hdl.handle.net/10198/9503 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Barreira, João C.M.; Oliveira, M. Beatriz P.P.; Ferreira, Isabel C.F.R. (2014). Development of a novel methodology for the analysis of ergosterol in mushrooms. Food Analytical Methods. ISSN 1936-9751. 7:1, p. 217-223 1936-9751 10.1007/s12161-013-9621-9 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799135232316669952 |