The role of microRNAs in amyotrophic lateral sclerosis

Detalhes bibliográficos
Autor(a) principal: Figueiredo, Joana Maria Serra de Oliveira Duarte
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/7991
Resumo: Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
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spelling The role of microRNAs in amyotrophic lateral sclerosisAmyotrophic Lateral sclerosis (ALS)Laser capture microdissection (LCM)MicroRNAs (miRNAs)RT-qPCRNeurodegenerationRNA qualityDissertação para obtenção do Grau de Mestre em Genética Molecular e BiomedicinaMicroRNAs (miRNAs) are emerging as a primary mediator of gene regulation in many different cell types. There is increasing evidence that specific subsets of miRNA play a prominent role in the nervous system, both in development and in specific neurodegenerative diseases. This study aims to elucidate the role of microRNA in selective motor neuron death that is the hallmark of amyotrophic Lateral sclerosis (ALS). Pre-symptomatic time-point was chosen since the levels of miRNAs are highly likely to be altered as a secondary consequence of cell injury and death in ALS. Laser capture microdissection (LCM) was used to study miRNA profiles in motor neurons of spinal cord tissue from SOD1G93A mice, the best characterized model of ALS. In preliminary work, using miRNA specific chips we have identified 2 miRNAs which are dramatically upregulated before disease onset. In this study, high RNA quality was achieved from laser captured cells, which consist in a major advance towards obtaining meaningful results of these miRNAs expression in downstream applications. Despite LCM technology has become increasingly sophisticated; rapidly obtaining enough amount of starting material for downstream applications is still extremely challenging. The combination of this optimized technique with microarrays, followed by RT-qPCR may provide insights into potential contribution of microRNAs to progression of neurodegeneration of motor neurons in ALS.Faculdade de Ciências e TecnologiaTalbot, KevinRUNFigueiredo, Joana Maria Serra de Oliveira Duarte2012-10-17T09:06:45Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/7991enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:40:21Zoai:run.unl.pt:10362/7991Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:17:52.362267Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The role of microRNAs in amyotrophic lateral sclerosis
title The role of microRNAs in amyotrophic lateral sclerosis
spellingShingle The role of microRNAs in amyotrophic lateral sclerosis
Figueiredo, Joana Maria Serra de Oliveira Duarte
Amyotrophic Lateral sclerosis (ALS)
Laser capture microdissection (LCM)
MicroRNAs (miRNAs)
RT-qPCR
Neurodegeneration
RNA quality
title_short The role of microRNAs in amyotrophic lateral sclerosis
title_full The role of microRNAs in amyotrophic lateral sclerosis
title_fullStr The role of microRNAs in amyotrophic lateral sclerosis
title_full_unstemmed The role of microRNAs in amyotrophic lateral sclerosis
title_sort The role of microRNAs in amyotrophic lateral sclerosis
author Figueiredo, Joana Maria Serra de Oliveira Duarte
author_facet Figueiredo, Joana Maria Serra de Oliveira Duarte
author_role author
dc.contributor.none.fl_str_mv Talbot, Kevin
RUN
dc.contributor.author.fl_str_mv Figueiredo, Joana Maria Serra de Oliveira Duarte
dc.subject.por.fl_str_mv Amyotrophic Lateral sclerosis (ALS)
Laser capture microdissection (LCM)
MicroRNAs (miRNAs)
RT-qPCR
Neurodegeneration
RNA quality
topic Amyotrophic Lateral sclerosis (ALS)
Laser capture microdissection (LCM)
MicroRNAs (miRNAs)
RT-qPCR
Neurodegeneration
RNA quality
description Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2012-10-17T09:06:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/7991
url http://hdl.handle.net/10362/7991
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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