Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://www.sciencedirect.com/science/article/pii/S2215017X20301892#! http://hdl.handle.net/10437/10408 |
Resumo: | Agar extraction from Gelidium and Gracilaria red seaweed species produces hundred thousand ton of carbohydrate-rich residues annually. Gelidium sesquipedale waste biomass obtained after agar extraction, still contained 44.2 % w/w total carbohydrates (dry-weight basis). These residues were biologically up-graded to poly-3-hydroxybutyrate (P3HB) after saccharification of their carbohydrate fraction to simple sugars. A combined hydrolysis treatment using sulfamic acid followed by enzymatic hydrolysis with cellulases produced a glucose-rich hydrolysate with a negligible content of inhibitors. With this treatment a sugar yield of circa 30 % (g glucose/g biomass) was attained. The algal hydrolysates were assessed as carbon source for the production of P3HB by the halotolerant bacteria Halomonas boliviensis. A cell concentration of 8.3 g L−1 containing 41 % (w/w) of polymer and a yield (YP/S) of 0.16 gpolymer/gglucose were attained in shake flask assays. In this work, cellulose-rich seaweed waste was shown to be an upgradable, sustainable source of carbohydrates. |
id |
RCAP_9b79511f8adb4f9790cc2f4fbed9da78 |
---|---|
oai_identifier_str |
oai:recil.ensinolusofona.pt:10437/10408 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensisBIOTECNOLOGIAGELIDIUM SESQUIPEDALEHALOMONAS BOLIVIENSISRESÍDUOS DE ALGAS MARINHASPOLI-3-HIDROXIBUTIRATORESÍDUOS DE MACROALGASENGENHARIA BIOTECNOLÓGICAALGASBIOTECHNOLOGYGELIDIUM SESQUIPEDALEHALOMONAS BOLIVIENSISSEAWEED RESIDUESPOLY-3-HYDROXYBUTYRATEMACROALGAE RESIDUESBIOTECHNOLOGY ENGINEERINGALGAEAgar extraction from Gelidium and Gracilaria red seaweed species produces hundred thousand ton of carbohydrate-rich residues annually. Gelidium sesquipedale waste biomass obtained after agar extraction, still contained 44.2 % w/w total carbohydrates (dry-weight basis). These residues were biologically up-graded to poly-3-hydroxybutyrate (P3HB) after saccharification of their carbohydrate fraction to simple sugars. A combined hydrolysis treatment using sulfamic acid followed by enzymatic hydrolysis with cellulases produced a glucose-rich hydrolysate with a negligible content of inhibitors. With this treatment a sugar yield of circa 30 % (g glucose/g biomass) was attained. The algal hydrolysates were assessed as carbon source for the production of P3HB by the halotolerant bacteria Halomonas boliviensis. A cell concentration of 8.3 g L−1 containing 41 % (w/w) of polymer and a yield (YP/S) of 0.16 gpolymer/gglucose were attained in shake flask assays. In this work, cellulose-rich seaweed waste was shown to be an upgradable, sustainable source of carbohydrates.Elsevier2020-08-31T11:05:34Z2020-09-01T00:00:00Z2020-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S2215017X20301892#!http://hdl.handle.net/10437/10408engTuna, StepanIzaguirre, JonBondar, MarinaMarques, MafaldaFernandes, Pedro Carlos de BarrosFonseca, Maria Manuela Regalo daCesário, Mariainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-09T14:05:13Zoai:recil.ensinolusofona.pt:10437/10408Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:13:01.262458Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis |
title |
Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis |
spellingShingle |
Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis Tuna, Stepan BIOTECNOLOGIA GELIDIUM SESQUIPEDALE HALOMONAS BOLIVIENSIS RESÍDUOS DE ALGAS MARINHAS POLI-3-HIDROXIBUTIRATO RESÍDUOS DE MACROALGAS ENGENHARIA BIOTECNOLÓGICA ALGAS BIOTECHNOLOGY GELIDIUM SESQUIPEDALE HALOMONAS BOLIVIENSIS SEAWEED RESIDUES POLY-3-HYDROXYBUTYRATE MACROALGAE RESIDUES BIOTECHNOLOGY ENGINEERING ALGAE |
title_short |
Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis |
title_full |
Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis |
title_fullStr |
Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis |
title_full_unstemmed |
Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis |
title_sort |
Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis |
author |
Tuna, Stepan |
author_facet |
Tuna, Stepan Izaguirre, Jon Bondar, Marina Marques, Mafalda Fernandes, Pedro Carlos de Barros Fonseca, Maria Manuela Regalo da Cesário, Maria |
author_role |
author |
author2 |
Izaguirre, Jon Bondar, Marina Marques, Mafalda Fernandes, Pedro Carlos de Barros Fonseca, Maria Manuela Regalo da Cesário, Maria |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Tuna, Stepan Izaguirre, Jon Bondar, Marina Marques, Mafalda Fernandes, Pedro Carlos de Barros Fonseca, Maria Manuela Regalo da Cesário, Maria |
dc.subject.por.fl_str_mv |
BIOTECNOLOGIA GELIDIUM SESQUIPEDALE HALOMONAS BOLIVIENSIS RESÍDUOS DE ALGAS MARINHAS POLI-3-HIDROXIBUTIRATO RESÍDUOS DE MACROALGAS ENGENHARIA BIOTECNOLÓGICA ALGAS BIOTECHNOLOGY GELIDIUM SESQUIPEDALE HALOMONAS BOLIVIENSIS SEAWEED RESIDUES POLY-3-HYDROXYBUTYRATE MACROALGAE RESIDUES BIOTECHNOLOGY ENGINEERING ALGAE |
topic |
BIOTECNOLOGIA GELIDIUM SESQUIPEDALE HALOMONAS BOLIVIENSIS RESÍDUOS DE ALGAS MARINHAS POLI-3-HIDROXIBUTIRATO RESÍDUOS DE MACROALGAS ENGENHARIA BIOTECNOLÓGICA ALGAS BIOTECHNOLOGY GELIDIUM SESQUIPEDALE HALOMONAS BOLIVIENSIS SEAWEED RESIDUES POLY-3-HYDROXYBUTYRATE MACROALGAE RESIDUES BIOTECHNOLOGY ENGINEERING ALGAE |
description |
Agar extraction from Gelidium and Gracilaria red seaweed species produces hundred thousand ton of carbohydrate-rich residues annually. Gelidium sesquipedale waste biomass obtained after agar extraction, still contained 44.2 % w/w total carbohydrates (dry-weight basis). These residues were biologically up-graded to poly-3-hydroxybutyrate (P3HB) after saccharification of their carbohydrate fraction to simple sugars. A combined hydrolysis treatment using sulfamic acid followed by enzymatic hydrolysis with cellulases produced a glucose-rich hydrolysate with a negligible content of inhibitors. With this treatment a sugar yield of circa 30 % (g glucose/g biomass) was attained. The algal hydrolysates were assessed as carbon source for the production of P3HB by the halotolerant bacteria Halomonas boliviensis. A cell concentration of 8.3 g L−1 containing 41 % (w/w) of polymer and a yield (YP/S) of 0.16 gpolymer/gglucose were attained in shake flask assays. In this work, cellulose-rich seaweed waste was shown to be an upgradable, sustainable source of carbohydrates. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-31T11:05:34Z 2020-09-01T00:00:00Z 2020-09 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.sciencedirect.com/science/article/pii/S2215017X20301892#! http://hdl.handle.net/10437/10408 |
url |
https://www.sciencedirect.com/science/article/pii/S2215017X20301892#! http://hdl.handle.net/10437/10408 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131227173683200 |