Facile preparation of mussel-inspired antibiotic-decorated titanium surfaces with enhanced antibacterial activity for implant applications

Detalhes bibliográficos
Autor(a) principal: Lee, Jae Seo
Data de Publicação: 2019
Outros Autores: Lee, Sang Jin, Yang, Seok Bin, Lee, Donghyun, Nah, Haram, Heo, Dong Nyoung, Moon, Ho-Jin, Hwang, Yu-Shik, Reis, R. L., Moon, Ji-Hoi, Kwon, Il Keun
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/62436
Resumo: Titanium implants (Ti) have been widely used in several medical fields. In clinical practice, Ti can become contaminated with bacteria through a variety of mechanisms. This contamination can lead to implant failure and serious infections. In this study, we aimed to develop a new, hybrid Ti with good biocompatibility and antibacterial properties by immobilizing ceftazidime (CFT) onto the Ti surface through polydopamine (PDA) and polyethyleneimine (PEI) chemistry. Hybrid Ti was confirmed by assessing the cell proliferation of human adipose-derived stem cells using a cell counting. The biofilm formation across the Ti surface of two bacterial strains associated with nosocomial infections, Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus, was evaluated by scanning electron microscopy. The viability of the bacteria exposed to Ti surface was evaluated by cell counting. Our results clearly demonstrate that the bacterial biofilm formation as well as bacterial viability was significantly reduced on the hybrid Ti as compared to the control, Ti alone. Collectively, the Ti surface was successfully modified to form the hybrid Ti exhibiting good biocompatibility and antibacterial properties through PDA, PEI, and CFT grafting. Within the limitations of this in vitro study, we conclude that the hybrid Ti may be useful for successful implant treatment.
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spelling Facile preparation of mussel-inspired antibiotic-decorated titanium surfaces with enhanced antibacterial activity for implant applicationsAntibacterial propertyBiocompatibilityCeftazidimePolydopamineTitanium implantScience & TechnologyTitanium implants (Ti) have been widely used in several medical fields. In clinical practice, Ti can become contaminated with bacteria through a variety of mechanisms. This contamination can lead to implant failure and serious infections. In this study, we aimed to develop a new, hybrid Ti with good biocompatibility and antibacterial properties by immobilizing ceftazidime (CFT) onto the Ti surface through polydopamine (PDA) and polyethyleneimine (PEI) chemistry. Hybrid Ti was confirmed by assessing the cell proliferation of human adipose-derived stem cells using a cell counting. The biofilm formation across the Ti surface of two bacterial strains associated with nosocomial infections, Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus, was evaluated by scanning electron microscopy. The viability of the bacteria exposed to Ti surface was evaluated by cell counting. Our results clearly demonstrate that the bacterial biofilm formation as well as bacterial viability was significantly reduced on the hybrid Ti as compared to the control, Ti alone. Collectively, the Ti surface was successfully modified to form the hybrid Ti exhibiting good biocompatibility and antibacterial properties through PDA, PEI, and CFT grafting. Within the limitations of this in vitro study, we conclude that the hybrid Ti may be useful for successful implant treatment.This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MSIT) (No. 2017M3A9E4048170).ElsevierUniversidade do MinhoLee, Jae SeoLee, Sang JinYang, Seok BinLee, DonghyunNah, HaramHeo, Dong NyoungMoon, Ho-JinHwang, Yu-ShikReis, R. L.Moon, Ji-HoiKwon, Il Keun2019-082019-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/62436engLee J. S., Lee S. J., Yang S. B., Lee D., Nah H., Heo D. N., Moon H. J., Hwang Y. - S., Reis R. L., Moon J. - H., Kwon I. K. Facile preparation of mussel-inspired antibiotic-decorated titanium surfaces with enhanced antibacterial activity for implant applications, Applied Surface Science, Vol. 496, Issue 1, pp. 143675, doi:10.1016/j.apsusc.2019.143675, 20190169-433210.1016/j.apsusc.2019.143675https://www.sciencedirect.com/science/article/pii/S0169433219324729#f0010info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:48Zoai:repositorium.sdum.uminho.pt:1822/62436Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:15:10.515128Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Facile preparation of mussel-inspired antibiotic-decorated titanium surfaces with enhanced antibacterial activity for implant applications
title Facile preparation of mussel-inspired antibiotic-decorated titanium surfaces with enhanced antibacterial activity for implant applications
spellingShingle Facile preparation of mussel-inspired antibiotic-decorated titanium surfaces with enhanced antibacterial activity for implant applications
Lee, Jae Seo
Antibacterial property
Biocompatibility
Ceftazidime
Polydopamine
Titanium implant
Science & Technology
title_short Facile preparation of mussel-inspired antibiotic-decorated titanium surfaces with enhanced antibacterial activity for implant applications
title_full Facile preparation of mussel-inspired antibiotic-decorated titanium surfaces with enhanced antibacterial activity for implant applications
title_fullStr Facile preparation of mussel-inspired antibiotic-decorated titanium surfaces with enhanced antibacterial activity for implant applications
title_full_unstemmed Facile preparation of mussel-inspired antibiotic-decorated titanium surfaces with enhanced antibacterial activity for implant applications
title_sort Facile preparation of mussel-inspired antibiotic-decorated titanium surfaces with enhanced antibacterial activity for implant applications
author Lee, Jae Seo
author_facet Lee, Jae Seo
Lee, Sang Jin
Yang, Seok Bin
Lee, Donghyun
Nah, Haram
Heo, Dong Nyoung
Moon, Ho-Jin
Hwang, Yu-Shik
Reis, R. L.
Moon, Ji-Hoi
Kwon, Il Keun
author_role author
author2 Lee, Sang Jin
Yang, Seok Bin
Lee, Donghyun
Nah, Haram
Heo, Dong Nyoung
Moon, Ho-Jin
Hwang, Yu-Shik
Reis, R. L.
Moon, Ji-Hoi
Kwon, Il Keun
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Lee, Jae Seo
Lee, Sang Jin
Yang, Seok Bin
Lee, Donghyun
Nah, Haram
Heo, Dong Nyoung
Moon, Ho-Jin
Hwang, Yu-Shik
Reis, R. L.
Moon, Ji-Hoi
Kwon, Il Keun
dc.subject.por.fl_str_mv Antibacterial property
Biocompatibility
Ceftazidime
Polydopamine
Titanium implant
Science & Technology
topic Antibacterial property
Biocompatibility
Ceftazidime
Polydopamine
Titanium implant
Science & Technology
description Titanium implants (Ti) have been widely used in several medical fields. In clinical practice, Ti can become contaminated with bacteria through a variety of mechanisms. This contamination can lead to implant failure and serious infections. In this study, we aimed to develop a new, hybrid Ti with good biocompatibility and antibacterial properties by immobilizing ceftazidime (CFT) onto the Ti surface through polydopamine (PDA) and polyethyleneimine (PEI) chemistry. Hybrid Ti was confirmed by assessing the cell proliferation of human adipose-derived stem cells using a cell counting. The biofilm formation across the Ti surface of two bacterial strains associated with nosocomial infections, Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus, was evaluated by scanning electron microscopy. The viability of the bacteria exposed to Ti surface was evaluated by cell counting. Our results clearly demonstrate that the bacterial biofilm formation as well as bacterial viability was significantly reduced on the hybrid Ti as compared to the control, Ti alone. Collectively, the Ti surface was successfully modified to form the hybrid Ti exhibiting good biocompatibility and antibacterial properties through PDA, PEI, and CFT grafting. Within the limitations of this in vitro study, we conclude that the hybrid Ti may be useful for successful implant treatment.
publishDate 2019
dc.date.none.fl_str_mv 2019-08
2019-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/62436
url http://hdl.handle.net/1822/62436
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lee J. S., Lee S. J., Yang S. B., Lee D., Nah H., Heo D. N., Moon H. J., Hwang Y. - S., Reis R. L., Moon J. - H., Kwon I. K. Facile preparation of mussel-inspired antibiotic-decorated titanium surfaces with enhanced antibacterial activity for implant applications, Applied Surface Science, Vol. 496, Issue 1, pp. 143675, doi:10.1016/j.apsusc.2019.143675, 2019
0169-4332
10.1016/j.apsusc.2019.143675
https://www.sciencedirect.com/science/article/pii/S0169433219324729#f0010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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