UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation

Detalhes bibliográficos
Autor(a) principal: SIDDIQUI,Masoom Raza
Data de Publicação: 2021
Outros Autores: HAKAMI,Afnan Ali Hussain, WABAIDUR,Saikh Mohammad, ALOTHMAN,Zeid Abdullah, KHAN,Moonis Ali, HUSAIN,Fohad Mabood
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Food Science and Technology (Campinas)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000100225
Resumo: ABSTRACT: Two analytical methods were developed, one utilizing sensitive UPLC-MS/MS method while other being the spectrophotometric method exploiting the Dushman reaction. Both methods were validated based on ICH guidelines and were successfully applied to quantitate the drug in marketed formulation. UPLC-MS/MS method responded linearly in the range of 1.6 µgmL-1-6.4 µgmL-1 while spectrophotometric procedure followed the Beer’s law in the concentration range of 30-100 µgmL-1. UPLC-MS/MS has been found to have a limit of quantitation of 0.97 µgmL-1 while spectrophotometric method was found to quantitate 14.00 µgmL-1 correctly with high precision. Both the methods showed an excellent recovery of 99.36%-100.91% (% RSD 1.05-1.73) and 98.64%-99.73% (%RSD 0.67-1.68) for UPLC-MS/MS and spectrophotometric, respectively.
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spelling UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulationCeftazidimeUPLC-MS/MSmethod developmentspectrophotometryABSTRACT: Two analytical methods were developed, one utilizing sensitive UPLC-MS/MS method while other being the spectrophotometric method exploiting the Dushman reaction. Both methods were validated based on ICH guidelines and were successfully applied to quantitate the drug in marketed formulation. UPLC-MS/MS method responded linearly in the range of 1.6 µgmL-1-6.4 µgmL-1 while spectrophotometric procedure followed the Beer’s law in the concentration range of 30-100 µgmL-1. UPLC-MS/MS has been found to have a limit of quantitation of 0.97 µgmL-1 while spectrophotometric method was found to quantitate 14.00 µgmL-1 correctly with high precision. Both the methods showed an excellent recovery of 99.36%-100.91% (% RSD 1.05-1.73) and 98.64%-99.73% (%RSD 0.67-1.68) for UPLC-MS/MS and spectrophotometric, respectively.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2021-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000100225Food Science and Technology v.41 n.1 2021reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.07020info:eu-repo/semantics/openAccessSIDDIQUI,Masoom RazaHAKAMI,Afnan Ali HussainWABAIDUR,Saikh MohammadALOTHMAN,Zeid AbdullahKHAN,Moonis AliHUSAIN,Fohad Maboodeng2021-03-18T00:00:00Zoai:scielo:S0101-20612021000100225Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2021-03-18T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false
dc.title.none.fl_str_mv UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation
title UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation
spellingShingle UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation
SIDDIQUI,Masoom Raza
Ceftazidime
UPLC-MS/MS
method development
spectrophotometry
title_short UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation
title_full UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation
title_fullStr UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation
title_full_unstemmed UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation
title_sort UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation
author SIDDIQUI,Masoom Raza
author_facet SIDDIQUI,Masoom Raza
HAKAMI,Afnan Ali Hussain
WABAIDUR,Saikh Mohammad
ALOTHMAN,Zeid Abdullah
KHAN,Moonis Ali
HUSAIN,Fohad Mabood
author_role author
author2 HAKAMI,Afnan Ali Hussain
WABAIDUR,Saikh Mohammad
ALOTHMAN,Zeid Abdullah
KHAN,Moonis Ali
HUSAIN,Fohad Mabood
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv SIDDIQUI,Masoom Raza
HAKAMI,Afnan Ali Hussain
WABAIDUR,Saikh Mohammad
ALOTHMAN,Zeid Abdullah
KHAN,Moonis Ali
HUSAIN,Fohad Mabood
dc.subject.por.fl_str_mv Ceftazidime
UPLC-MS/MS
method development
spectrophotometry
topic Ceftazidime
UPLC-MS/MS
method development
spectrophotometry
description ABSTRACT: Two analytical methods were developed, one utilizing sensitive UPLC-MS/MS method while other being the spectrophotometric method exploiting the Dushman reaction. Both methods were validated based on ICH guidelines and were successfully applied to quantitate the drug in marketed formulation. UPLC-MS/MS method responded linearly in the range of 1.6 µgmL-1-6.4 µgmL-1 while spectrophotometric procedure followed the Beer’s law in the concentration range of 30-100 µgmL-1. UPLC-MS/MS has been found to have a limit of quantitation of 0.97 µgmL-1 while spectrophotometric method was found to quantitate 14.00 µgmL-1 correctly with high precision. Both the methods showed an excellent recovery of 99.36%-100.91% (% RSD 1.05-1.73) and 98.64%-99.73% (%RSD 0.67-1.68) for UPLC-MS/MS and spectrophotometric, respectively.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000100225
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000100225
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/fst.07020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv Food Science and Technology v.41 n.1 2021
reponame:Food Science and Technology (Campinas)
instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron:SBCTA
instname_str Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron_str SBCTA
institution SBCTA
reponame_str Food Science and Technology (Campinas)
collection Food Science and Technology (Campinas)
repository.name.fl_str_mv Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
repository.mail.fl_str_mv ||revista@sbcta.org.br
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