Clenbuterol Storage Stability in the Bovine Urine and Liver Samples Used for European Official Control in the Azores Islands (Portugal)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/10327 https://doi.org/10.1021/jf802995e |
Resumo: | Clenbuterol is a well-known growth promoter, illegally used in farm animals, especially in cattle. Samples collected for the screening of β2-agonist residues in Portuguese Azores Islands must travel through all the nine islands until they reach Azores Central Laboratory. If any suspicious sample is detected, it must be further transported to the National Reference Laboratory in Lisbon for confirmation. As a consequence of these circumstances, samples are submitted to different transport and storage times, as well as different temperature conditions and in some cases successive freezing and thawing cycles. As clenbuterol is the most detected β2-agonist growth promoter in the Portuguese Residue Monitoring Plan, studies were conducted on the stability of this compound in incurred samples (bovine liver and urine) at +4, −20 and −60 °C over time. Samples kept at −20 °C were also analyzed over time after successive freezing and thawing cycles. The analyses of clenbuterol over time were performed by gas chromatography−mass spectrometry (GC−MS) with selected ion monitoring (SIM). Clenbuterol in incurred urine and liver samples was significantly stable up to 20 weeks at −20 and −60 °C and after, at least, six consecutive freezings and thawings. At +4 °C, clenbuterol remained stable, at least until 12 weeks in urine and up to 20 weeks in liver. |
id |
RCAP_9bf7b2f78ee4fac08249b8482b057f83 |
---|---|
oai_identifier_str |
oai:estudogeral.uc.pt:10316/10327 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Clenbuterol Storage Stability in the Bovine Urine and Liver Samples Used for European Official Control in the Azores Islands (Portugal)ClenbuterolUrineLiverStabilityGC-MSClenbuterol is a well-known growth promoter, illegally used in farm animals, especially in cattle. Samples collected for the screening of β2-agonist residues in Portuguese Azores Islands must travel through all the nine islands until they reach Azores Central Laboratory. If any suspicious sample is detected, it must be further transported to the National Reference Laboratory in Lisbon for confirmation. As a consequence of these circumstances, samples are submitted to different transport and storage times, as well as different temperature conditions and in some cases successive freezing and thawing cycles. As clenbuterol is the most detected β2-agonist growth promoter in the Portuguese Residue Monitoring Plan, studies were conducted on the stability of this compound in incurred samples (bovine liver and urine) at +4, −20 and −60 °C over time. Samples kept at −20 °C were also analyzed over time after successive freezing and thawing cycles. The analyses of clenbuterol over time were performed by gas chromatography−mass spectrometry (GC−MS) with selected ion monitoring (SIM). Clenbuterol in incurred urine and liver samples was significantly stable up to 20 weeks at −20 and −60 °C and after, at least, six consecutive freezings and thawings. At +4 °C, clenbuterol remained stable, at least until 12 weeks in urine and up to 20 weeks in liver.American Chemical Society2009-02-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/10327http://hdl.handle.net/10316/10327https://doi.org/10.1021/jf802995eengJournal of Agricultural and Food Chemistry. 57:3 (2009) 910-9140021-8561Pinheiro, IsabelJesuíno, BrunoBarbosa, JorgeFerreira, HumbertoRamos, FernandoMatos, JoséSilveira, Maria Irene Noronha dainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-05-27T16:14:35Zoai:estudogeral.uc.pt:10316/10327Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:47:24.275621Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Clenbuterol Storage Stability in the Bovine Urine and Liver Samples Used for European Official Control in the Azores Islands (Portugal) |
title |
Clenbuterol Storage Stability in the Bovine Urine and Liver Samples Used for European Official Control in the Azores Islands (Portugal) |
spellingShingle |
Clenbuterol Storage Stability in the Bovine Urine and Liver Samples Used for European Official Control in the Azores Islands (Portugal) Pinheiro, Isabel Clenbuterol Urine Liver Stability GC-MS |
title_short |
Clenbuterol Storage Stability in the Bovine Urine and Liver Samples Used for European Official Control in the Azores Islands (Portugal) |
title_full |
Clenbuterol Storage Stability in the Bovine Urine and Liver Samples Used for European Official Control in the Azores Islands (Portugal) |
title_fullStr |
Clenbuterol Storage Stability in the Bovine Urine and Liver Samples Used for European Official Control in the Azores Islands (Portugal) |
title_full_unstemmed |
Clenbuterol Storage Stability in the Bovine Urine and Liver Samples Used for European Official Control in the Azores Islands (Portugal) |
title_sort |
Clenbuterol Storage Stability in the Bovine Urine and Liver Samples Used for European Official Control in the Azores Islands (Portugal) |
author |
Pinheiro, Isabel |
author_facet |
Pinheiro, Isabel Jesuíno, Bruno Barbosa, Jorge Ferreira, Humberto Ramos, Fernando Matos, José Silveira, Maria Irene Noronha da |
author_role |
author |
author2 |
Jesuíno, Bruno Barbosa, Jorge Ferreira, Humberto Ramos, Fernando Matos, José Silveira, Maria Irene Noronha da |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Pinheiro, Isabel Jesuíno, Bruno Barbosa, Jorge Ferreira, Humberto Ramos, Fernando Matos, José Silveira, Maria Irene Noronha da |
dc.subject.por.fl_str_mv |
Clenbuterol Urine Liver Stability GC-MS |
topic |
Clenbuterol Urine Liver Stability GC-MS |
description |
Clenbuterol is a well-known growth promoter, illegally used in farm animals, especially in cattle. Samples collected for the screening of β2-agonist residues in Portuguese Azores Islands must travel through all the nine islands until they reach Azores Central Laboratory. If any suspicious sample is detected, it must be further transported to the National Reference Laboratory in Lisbon for confirmation. As a consequence of these circumstances, samples are submitted to different transport and storage times, as well as different temperature conditions and in some cases successive freezing and thawing cycles. As clenbuterol is the most detected β2-agonist growth promoter in the Portuguese Residue Monitoring Plan, studies were conducted on the stability of this compound in incurred samples (bovine liver and urine) at +4, −20 and −60 °C over time. Samples kept at −20 °C were also analyzed over time after successive freezing and thawing cycles. The analyses of clenbuterol over time were performed by gas chromatography−mass spectrometry (GC−MS) with selected ion monitoring (SIM). Clenbuterol in incurred urine and liver samples was significantly stable up to 20 weeks at −20 and −60 °C and after, at least, six consecutive freezings and thawings. At +4 °C, clenbuterol remained stable, at least until 12 weeks in urine and up to 20 weeks in liver. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-02-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/10327 http://hdl.handle.net/10316/10327 https://doi.org/10.1021/jf802995e |
url |
http://hdl.handle.net/10316/10327 https://doi.org/10.1021/jf802995e |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Agricultural and Food Chemistry. 57:3 (2009) 910-914 0021-8561 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133751163224064 |