ABC Efflux Transporters and the Circuitry of miRNAs
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/97345 |
Resumo: | This research was funded by Fundação de Ciência e Tecnologia (FCT), grant number UID-BIM-00009-2020 and GHTM-UID/Multi/04413/2013. The APC was funded by Fundação de Ciência e Tecnologia (FCT), grant number UID-BIM-00009-2020. |
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ABC Efflux Transporters and the Circuitry of miRNAsKinetics of Expression in Cancer Drug Resistancecancer drug resistancemicroRNAsgene regulationABC drug transportersABCB1MDR1doxorubicinSDG 3 - Good Health and Well-beingThis research was funded by Fundação de Ciência e Tecnologia (FCT), grant number UID-BIM-00009-2020 and GHTM-UID/Multi/04413/2013. The APC was funded by Fundação de Ciência e Tecnologia (FCT), grant number UID-BIM-00009-2020.Cancer drug resistance (CDR) is a major problem in therapeutic failure. Over 90% of patients with metastatic cancer present CDR. Several mechanisms underlie CDR, including the increased expression of efflux ABC transporters and epigenetic phenomena. Nevertheless, a topic that is not usually addressed is the mechanism underlying the loss of CDR once the challenge to these cells is withdrawn. A KCR cell line (doxorubicin-resistant, expressing ABCB1) was used to induce loss of resistance by withdrawing doxorubicin in culture medium. ABCB1 activity was analysed by fluorescence microscopy and flow cytometry through substrate (DiOC2) retention assays. The expression of 1008 microRNAs was assessed before and after doxorubicin withdrawal. After 16 weeks of doxorubicin withdrawal, a decrease of ABCB1 activity and expression occurred. Moreover, we determined a signature of 23 microRNAs, 13 underexpressed and 10 overexpressed, as a tool to assess loss of resistance. Through pathway enrichment analysis, “Pathways in cancer”, “Proteoglycans in cancer” and “ECM-receptor interaction” were identified as relevant in the loss of CDR. Taken together, the data reinforce the assumption that ABCB1 plays a major role in the kinetics of CDR, and their levels of expression are in the dependence of the circuitry of cell miRNAsCentre for Toxicogenomics and Human Health (ToxOmics)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)RUNGomes, Bruno C.Honrado, MónicaArmada, AnaViveiros, MiguelRueff, JoséRodrigues, António S.2020-05-07T22:53:57Z2020-04-232020-04-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/97345eng1422-0067PURE: 17890939https://doi.org/10.3390/ijms21082985info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:44:27Zoai:run.unl.pt:10362/97345Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:38:42.883936Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
ABC Efflux Transporters and the Circuitry of miRNAs Kinetics of Expression in Cancer Drug Resistance |
| title |
ABC Efflux Transporters and the Circuitry of miRNAs |
| spellingShingle |
ABC Efflux Transporters and the Circuitry of miRNAs Gomes, Bruno C. cancer drug resistance microRNAs gene regulation ABC drug transporters ABCB1 MDR1 doxorubicin SDG 3 - Good Health and Well-being |
| title_short |
ABC Efflux Transporters and the Circuitry of miRNAs |
| title_full |
ABC Efflux Transporters and the Circuitry of miRNAs |
| title_fullStr |
ABC Efflux Transporters and the Circuitry of miRNAs |
| title_full_unstemmed |
ABC Efflux Transporters and the Circuitry of miRNAs |
| title_sort |
ABC Efflux Transporters and the Circuitry of miRNAs |
| author |
Gomes, Bruno C. |
| author_facet |
Gomes, Bruno C. Honrado, Mónica Armada, Ana Viveiros, Miguel Rueff, José Rodrigues, António S. |
| author_role |
author |
| author2 |
Honrado, Mónica Armada, Ana Viveiros, Miguel Rueff, José Rodrigues, António S. |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Centre for Toxicogenomics and Human Health (ToxOmics) NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) RUN |
| dc.contributor.author.fl_str_mv |
Gomes, Bruno C. Honrado, Mónica Armada, Ana Viveiros, Miguel Rueff, José Rodrigues, António S. |
| dc.subject.por.fl_str_mv |
cancer drug resistance microRNAs gene regulation ABC drug transporters ABCB1 MDR1 doxorubicin SDG 3 - Good Health and Well-being |
| topic |
cancer drug resistance microRNAs gene regulation ABC drug transporters ABCB1 MDR1 doxorubicin SDG 3 - Good Health and Well-being |
| description |
This research was funded by Fundação de Ciência e Tecnologia (FCT), grant number UID-BIM-00009-2020 and GHTM-UID/Multi/04413/2013. The APC was funded by Fundação de Ciência e Tecnologia (FCT), grant number UID-BIM-00009-2020. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-05-07T22:53:57Z 2020-04-23 2020-04-23T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/97345 |
| url |
http://hdl.handle.net/10362/97345 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1422-0067 PURE: 17890939 https://doi.org/10.3390/ijms21082985 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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