In vitro propagation of insectivorous plants for phytochemical purposes

Detalhes bibliográficos
Autor(a) principal: Coelho, Natacha
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/457
Resumo: Dissertação mest., Engenharia Biológica, Universidade do Algarve, 2009
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spelling In vitro propagation of insectivorous plants for phytochemical purposesTesesBioquímicaBiotecnologiaPlantas insectívorasPinguicula lusitanicaDroseraDissertação mest., Engenharia Biológica, Universidade do Algarve, 2009The aims of this research work were to establish in vitro propagation protocols for the insectivorous plants D. intermedia, D. rotundifolia and P. vulgaris, using seeds as explants, in order to produce plants for biocompound extraction and to replenish natural populations. The antioxidant capacity of the extracts prepared from in vitro cultures of these species was evaluated. The establishment of a simple protocol for in vitro propagation of D. intermedia is reported. Protocols for micropropagation of P. vulgaris and D. rotundifolia were initiated but still need to be improved. Cultures of all three species were initiated from shoots of in vitro produced seedlings. The effect of three MS medium concentrations, ¼MS, ½MS and MS, without PGR were tested in D. intermedia and P. vulgaris. The same media supplemented with 0.1 mg l-1 KIN were investigated in D. intermedia and ¼MS supplemented with 0.5 mg l-1 KIN or ZEA were investigated in P. vulgaris. In all media D. intermedia shoots rooted during the proliferation phase, presenting a multiplication and rooting percentage above 90%. The highest mean number of shoots was attained in ¼MS and ¼MS supplemented with 0.1 mg l-1 KIN. All the D. intermedia plants were successfully acclimatized to ex vitro conditions. The formation of roots also occurred during P. vulgaris proliferation phase, though with poorer results for both proliferation and rooting. Nevertheless, the best results were obtained in the media supplemented with cytokinins. In D. rotundifolia the media tested were ¼MS and MS. Multiplication percentage and mean number of shoots was higher in ¼MS medium and no rooting was observed. Antioxidant activity of D. intermedia, D. rotundifolia and P. lusitanica extracts was measured using three different methods: ORAC, F-C phenolics and TEAC. Methanol and standard maceration improved the efficiency of the antioxidant extraction procedure. For all tested methods P. lusitanica presented the highest antioxidant activity, representing a possible good source of antioxidants.Romano, AnabelaGonçalves, SandraSapientiaCoelho, Natacha2011-09-07T16:04:45Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.1/457eng577 COE*In Caveinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:11:13Zoai:sapientia.ualg.pt:10400.1/457Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:54:49.715107Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In vitro propagation of insectivorous plants for phytochemical purposes
title In vitro propagation of insectivorous plants for phytochemical purposes
spellingShingle In vitro propagation of insectivorous plants for phytochemical purposes
Coelho, Natacha
Teses
Bioquímica
Biotecnologia
Plantas insectívoras
Pinguicula lusitanica
Drosera
title_short In vitro propagation of insectivorous plants for phytochemical purposes
title_full In vitro propagation of insectivorous plants for phytochemical purposes
title_fullStr In vitro propagation of insectivorous plants for phytochemical purposes
title_full_unstemmed In vitro propagation of insectivorous plants for phytochemical purposes
title_sort In vitro propagation of insectivorous plants for phytochemical purposes
author Coelho, Natacha
author_facet Coelho, Natacha
author_role author
dc.contributor.none.fl_str_mv Romano, Anabela
Gonçalves, Sandra
Sapientia
dc.contributor.author.fl_str_mv Coelho, Natacha
dc.subject.por.fl_str_mv Teses
Bioquímica
Biotecnologia
Plantas insectívoras
Pinguicula lusitanica
Drosera
topic Teses
Bioquímica
Biotecnologia
Plantas insectívoras
Pinguicula lusitanica
Drosera
description Dissertação mest., Engenharia Biológica, Universidade do Algarve, 2009
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01T00:00:00Z
2011-09-07T16:04:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/457
url http://hdl.handle.net/10400.1/457
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 577 COE*In Cave
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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application/pdf
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.mail.fl_str_mv
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