Co infection of OMMV and OLV-1 enhances symptoms and increases both viruses accumulation and viral derived siRNAs in plants
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/28166 |
Resumo: | Previous extensive field surveys in olive orchards have revealed high levels of Olive mild mosaic virus (OMMV) and Olive latent virus 1 (OLV-1), frequently appearing in mixed infections. These viruses belong to genus Alphanecrovirus and their RNA dependent RNA polymerase (RdRp),as well as their p6 and p8 amino acid sequences share over 87% identity. Preliminary studies have shown that co infection of OMMV and OLV-1 is associated to an intensification of symptoms, as well to an increase in transmission efficiency, suggesting a synergistic effect. Single and double infections of OMMV and OLV-1 were obtained through mechanical inoculation of Nicotiana benthamiana plants and the second upper leaf from each inoculated plant was collected at different stages and used for quantitative PCR. In this study we found that the co infection of OMMV and OLV-1 causes an exacerbation of symptoms and increases the accumulation of both viruses in N. benthamiana plants. Highthroughput sequencing of siRNAs from both viruses in singly and co infected plants showed that OMMV and OLV-1 co infection increased the accumulation of siRNAs, mainly of 21 and 22 nt in length, with most non distinguishable between OMMV and OLV-1 siRNAs. Our findings suggest that siRNAs of both viruses have possible roles in the synergistic interaction between OLV-1 and OMMV in N. benthamiana plants. Whether a similar situation occurs in olive fields is not yet known and studies are being pursued. |
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Co infection of OMMV and OLV-1 enhances symptoms and increases both viruses accumulation and viral derived siRNAs in plantsco infectionvirussilencingPrevious extensive field surveys in olive orchards have revealed high levels of Olive mild mosaic virus (OMMV) and Olive latent virus 1 (OLV-1), frequently appearing in mixed infections. These viruses belong to genus Alphanecrovirus and their RNA dependent RNA polymerase (RdRp),as well as their p6 and p8 amino acid sequences share over 87% identity. Preliminary studies have shown that co infection of OMMV and OLV-1 is associated to an intensification of symptoms, as well to an increase in transmission efficiency, suggesting a synergistic effect. Single and double infections of OMMV and OLV-1 were obtained through mechanical inoculation of Nicotiana benthamiana plants and the second upper leaf from each inoculated plant was collected at different stages and used for quantitative PCR. In this study we found that the co infection of OMMV and OLV-1 causes an exacerbation of symptoms and increases the accumulation of both viruses in N. benthamiana plants. Highthroughput sequencing of siRNAs from both viruses in singly and co infected plants showed that OMMV and OLV-1 co infection increased the accumulation of siRNAs, mainly of 21 and 22 nt in length, with most non distinguishable between OMMV and OLV-1 siRNAs. Our findings suggest that siRNAs of both viruses have possible roles in the synergistic interaction between OLV-1 and OMMV in N. benthamiana plants. Whether a similar situation occurs in olive fields is not yet known and studies are being pursued.2020-10-23T10:00:39Z2020-10-232019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/28166http://hdl.handle.net/10174/28166engVaranda C.M.R.; Materatski, P.; Campos, M.D.; Clara, M.I.; Marques, N.; Nolasco, G.; Félix, M.R. Co infection of OMMV and OLV-1 enhances symptoms and increases both viruses accumulation and viral derived siRNAs in plants. (2019). IS-MPMI XVIII congress, July 14 – 18, Glasgow, Scotland.naonaosimcarlavaranda@uevora.ptpmateratski@uevora.ptmdcc@uevora.pticlara@uevora.ptmrff@uevora.pt581Varanda, CarlaMateratski, PatrickCampos, MariaClara, Maria IvoneFélix, Maria Rosárioinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:23:59Zoai:dspace.uevora.pt:10174/28166Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:17:59.258107Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Co infection of OMMV and OLV-1 enhances symptoms and increases both viruses accumulation and viral derived siRNAs in plants |
title |
Co infection of OMMV and OLV-1 enhances symptoms and increases both viruses accumulation and viral derived siRNAs in plants |
spellingShingle |
Co infection of OMMV and OLV-1 enhances symptoms and increases both viruses accumulation and viral derived siRNAs in plants Varanda, Carla co infection virus silencing |
title_short |
Co infection of OMMV and OLV-1 enhances symptoms and increases both viruses accumulation and viral derived siRNAs in plants |
title_full |
Co infection of OMMV and OLV-1 enhances symptoms and increases both viruses accumulation and viral derived siRNAs in plants |
title_fullStr |
Co infection of OMMV and OLV-1 enhances symptoms and increases both viruses accumulation and viral derived siRNAs in plants |
title_full_unstemmed |
Co infection of OMMV and OLV-1 enhances symptoms and increases both viruses accumulation and viral derived siRNAs in plants |
title_sort |
Co infection of OMMV and OLV-1 enhances symptoms and increases both viruses accumulation and viral derived siRNAs in plants |
author |
Varanda, Carla |
author_facet |
Varanda, Carla Materatski, Patrick Campos, Maria Clara, Maria Ivone Félix, Maria Rosário |
author_role |
author |
author2 |
Materatski, Patrick Campos, Maria Clara, Maria Ivone Félix, Maria Rosário |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Varanda, Carla Materatski, Patrick Campos, Maria Clara, Maria Ivone Félix, Maria Rosário |
dc.subject.por.fl_str_mv |
co infection virus silencing |
topic |
co infection virus silencing |
description |
Previous extensive field surveys in olive orchards have revealed high levels of Olive mild mosaic virus (OMMV) and Olive latent virus 1 (OLV-1), frequently appearing in mixed infections. These viruses belong to genus Alphanecrovirus and their RNA dependent RNA polymerase (RdRp),as well as their p6 and p8 amino acid sequences share over 87% identity. Preliminary studies have shown that co infection of OMMV and OLV-1 is associated to an intensification of symptoms, as well to an increase in transmission efficiency, suggesting a synergistic effect. Single and double infections of OMMV and OLV-1 were obtained through mechanical inoculation of Nicotiana benthamiana plants and the second upper leaf from each inoculated plant was collected at different stages and used for quantitative PCR. In this study we found that the co infection of OMMV and OLV-1 causes an exacerbation of symptoms and increases the accumulation of both viruses in N. benthamiana plants. Highthroughput sequencing of siRNAs from both viruses in singly and co infected plants showed that OMMV and OLV-1 co infection increased the accumulation of siRNAs, mainly of 21 and 22 nt in length, with most non distinguishable between OMMV and OLV-1 siRNAs. Our findings suggest that siRNAs of both viruses have possible roles in the synergistic interaction between OLV-1 and OMMV in N. benthamiana plants. Whether a similar situation occurs in olive fields is not yet known and studies are being pursued. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01T00:00:00Z 2020-10-23T10:00:39Z 2020-10-23 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/28166 http://hdl.handle.net/10174/28166 |
url |
http://hdl.handle.net/10174/28166 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Varanda C.M.R.; Materatski, P.; Campos, M.D.; Clara, M.I.; Marques, N.; Nolasco, G.; Félix, M.R. Co infection of OMMV and OLV-1 enhances symptoms and increases both viruses accumulation and viral derived siRNAs in plants. (2019). IS-MPMI XVIII congress, July 14 – 18, Glasgow, Scotland. nao nao sim carlavaranda@uevora.pt pmateratski@uevora.pt mdcc@uevora.pt iclara@uevora.pt mrff@uevora.pt 581 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136661786853376 |