Toxic effects of tamoxifen on the growth and respiratory activity of Bacillus stearothermophilus
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5801 https://doi.org/10.1016/S0887-2333(01)00024-8 |
Resumo: | The anticancer drug tamoxifen (TAM) is used as first line therapy in breast cancer. Although tamoxifen is usually considered an estrogen antagonist, several studies suggest alternative mechanisms of action. Bacillus stearothermophilus has been used as a model to clarify the antiproliferative action of tamoxifen putatively related with drug-membrane interaction. According to previous data, TAM induces perturbation of membrane structure along with impairment of bacterial growth. The aim of this work was to correlate the effects of TAM on growth of intact B. stearothermophilus with the respiratory activity of isolated protoplasts of this bacteria. TAM inhibits bacterial growth and oxygen consumption of protoplasts as a function of concentration. Effects on oxygen consumption depend on the substrate used: NADH, allowing to study the full respiratory chain and ascorbate-TMPD to probe the final oxidase segment. The interaction of TAM with the respiratory components occurs at a level preceding the cytochrome oxidase segment. |
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Toxic effects of tamoxifen on the growth and respiratory activity of Bacillus stearothermophilusTamoxifenBacillus stearothermophilusBacterial growthRespiratory activityThe anticancer drug tamoxifen (TAM) is used as first line therapy in breast cancer. Although tamoxifen is usually considered an estrogen antagonist, several studies suggest alternative mechanisms of action. Bacillus stearothermophilus has been used as a model to clarify the antiproliferative action of tamoxifen putatively related with drug-membrane interaction. According to previous data, TAM induces perturbation of membrane structure along with impairment of bacterial growth. The aim of this work was to correlate the effects of TAM on growth of intact B. stearothermophilus with the respiratory activity of isolated protoplasts of this bacteria. TAM inhibits bacterial growth and oxygen consumption of protoplasts as a function of concentration. Effects on oxygen consumption depend on the substrate used: NADH, allowing to study the full respiratory chain and ascorbate-TMPD to probe the final oxidase segment. The interaction of TAM with the respiratory components occurs at a level preceding the cytochrome oxidase segment.http://www.sciencedirect.com/science/article/B6TCP-440BK3X-9/1/8b03168c17d58852c8008488f75854cc2001info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5801http://hdl.handle.net/10316/5801https://doi.org/10.1016/S0887-2333(01)00024-8engToxicology in Vitro. 15:4-5 (2001) 303-305Luxo, C.Jurado, A. S.Custódio, J. B. A.Madeira, V. M. C.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-09-23T08:56:54Zoai:estudogeral.uc.pt:10316/5801Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:47:18.833609Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Toxic effects of tamoxifen on the growth and respiratory activity of Bacillus stearothermophilus |
title |
Toxic effects of tamoxifen on the growth and respiratory activity of Bacillus stearothermophilus |
spellingShingle |
Toxic effects of tamoxifen on the growth and respiratory activity of Bacillus stearothermophilus Luxo, C. Tamoxifen Bacillus stearothermophilus Bacterial growth Respiratory activity |
title_short |
Toxic effects of tamoxifen on the growth and respiratory activity of Bacillus stearothermophilus |
title_full |
Toxic effects of tamoxifen on the growth and respiratory activity of Bacillus stearothermophilus |
title_fullStr |
Toxic effects of tamoxifen on the growth and respiratory activity of Bacillus stearothermophilus |
title_full_unstemmed |
Toxic effects of tamoxifen on the growth and respiratory activity of Bacillus stearothermophilus |
title_sort |
Toxic effects of tamoxifen on the growth and respiratory activity of Bacillus stearothermophilus |
author |
Luxo, C. |
author_facet |
Luxo, C. Jurado, A. S. Custódio, J. B. A. Madeira, V. M. C. |
author_role |
author |
author2 |
Jurado, A. S. Custódio, J. B. A. Madeira, V. M. C. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Luxo, C. Jurado, A. S. Custódio, J. B. A. Madeira, V. M. C. |
dc.subject.por.fl_str_mv |
Tamoxifen Bacillus stearothermophilus Bacterial growth Respiratory activity |
topic |
Tamoxifen Bacillus stearothermophilus Bacterial growth Respiratory activity |
description |
The anticancer drug tamoxifen (TAM) is used as first line therapy in breast cancer. Although tamoxifen is usually considered an estrogen antagonist, several studies suggest alternative mechanisms of action. Bacillus stearothermophilus has been used as a model to clarify the antiproliferative action of tamoxifen putatively related with drug-membrane interaction. According to previous data, TAM induces perturbation of membrane structure along with impairment of bacterial growth. The aim of this work was to correlate the effects of TAM on growth of intact B. stearothermophilus with the respiratory activity of isolated protoplasts of this bacteria. TAM inhibits bacterial growth and oxygen consumption of protoplasts as a function of concentration. Effects on oxygen consumption depend on the substrate used: NADH, allowing to study the full respiratory chain and ascorbate-TMPD to probe the final oxidase segment. The interaction of TAM with the respiratory components occurs at a level preceding the cytochrome oxidase segment. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5801 http://hdl.handle.net/10316/5801 https://doi.org/10.1016/S0887-2333(01)00024-8 |
url |
http://hdl.handle.net/10316/5801 https://doi.org/10.1016/S0887-2333(01)00024-8 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxicology in Vitro. 15:4-5 (2001) 303-305 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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