An electrical method to measure low-frequency collective and synchronized cell activity using extracellular electrodes
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/108920 https://doi.org/10.1016/j.sbsr.2016.06.002 |
Resumo: | An electrical method to measure extracellular bioelectrical activity in vitro is presented. This method exploits the Helmholtz capacitive double-layer established at the electrode surface. Small extracellular voltage variations in the order of μVs induce through the double-layer capacitor a displacement current that is measured. This current is then enhanced by a gain factor proportional to the electrode capacitance. In addition, when measurements are carried out at lowfrequencies in currentmode the electrode contribution to the noise can be minimized. The performance of the electrodes and themethod is demonstrated using zebrafish hearts and glioma cell cultures.We propose that this electricalmethod is an ideal tool to measure in vitro slow and temporally synchronized events that are often involved in long range intracellular signaling. |
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7160 |
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An electrical method to measure low-frequency collective and synchronized cell activity using extracellular electrodesExtracellular electrodesBioelectrical signalsDisplacement currentElectrical double-layerIntercellular signalingAn electrical method to measure extracellular bioelectrical activity in vitro is presented. This method exploits the Helmholtz capacitive double-layer established at the electrode surface. Small extracellular voltage variations in the order of μVs induce through the double-layer capacitor a displacement current that is measured. This current is then enhanced by a gain factor proportional to the electrode capacitance. In addition, when measurements are carried out at lowfrequencies in currentmode the electrode contribution to the noise can be minimized. The performance of the electrodes and themethod is demonstrated using zebrafish hearts and glioma cell cultures.We propose that this electricalmethod is an ideal tool to measure in vitro slow and temporally synchronized events that are often involved in long range intracellular signaling.Elsevier2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/108920http://hdl.handle.net/10316/108920https://doi.org/10.1016/j.sbsr.2016.06.002eng22141804Medeiros, Maria C. R.Mestre, AnaInácio, PedroAsgarif, SanazAraújo, Inês M.Hubbard, Peter C.Velez, ZéliaCancela, M. LeonorRocha, Paulo R. F.de Leeuw, Dago M.Biscarini, FabioGomes, Henrique L.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-09-25T09:50:11Zoai:estudogeral.uc.pt:10316/108920Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:25:09.442464Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
An electrical method to measure low-frequency collective and synchronized cell activity using extracellular electrodes |
title |
An electrical method to measure low-frequency collective and synchronized cell activity using extracellular electrodes |
spellingShingle |
An electrical method to measure low-frequency collective and synchronized cell activity using extracellular electrodes Medeiros, Maria C. R. Extracellular electrodes Bioelectrical signals Displacement current Electrical double-layer Intercellular signaling |
title_short |
An electrical method to measure low-frequency collective and synchronized cell activity using extracellular electrodes |
title_full |
An electrical method to measure low-frequency collective and synchronized cell activity using extracellular electrodes |
title_fullStr |
An electrical method to measure low-frequency collective and synchronized cell activity using extracellular electrodes |
title_full_unstemmed |
An electrical method to measure low-frequency collective and synchronized cell activity using extracellular electrodes |
title_sort |
An electrical method to measure low-frequency collective and synchronized cell activity using extracellular electrodes |
author |
Medeiros, Maria C. R. |
author_facet |
Medeiros, Maria C. R. Mestre, Ana Inácio, Pedro Asgarif, Sanaz Araújo, Inês M. Hubbard, Peter C. Velez, Zélia Cancela, M. Leonor Rocha, Paulo R. F. de Leeuw, Dago M. Biscarini, Fabio Gomes, Henrique L. |
author_role |
author |
author2 |
Mestre, Ana Inácio, Pedro Asgarif, Sanaz Araújo, Inês M. Hubbard, Peter C. Velez, Zélia Cancela, M. Leonor Rocha, Paulo R. F. de Leeuw, Dago M. Biscarini, Fabio Gomes, Henrique L. |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Medeiros, Maria C. R. Mestre, Ana Inácio, Pedro Asgarif, Sanaz Araújo, Inês M. Hubbard, Peter C. Velez, Zélia Cancela, M. Leonor Rocha, Paulo R. F. de Leeuw, Dago M. Biscarini, Fabio Gomes, Henrique L. |
dc.subject.por.fl_str_mv |
Extracellular electrodes Bioelectrical signals Displacement current Electrical double-layer Intercellular signaling |
topic |
Extracellular electrodes Bioelectrical signals Displacement current Electrical double-layer Intercellular signaling |
description |
An electrical method to measure extracellular bioelectrical activity in vitro is presented. This method exploits the Helmholtz capacitive double-layer established at the electrode surface. Small extracellular voltage variations in the order of μVs induce through the double-layer capacitor a displacement current that is measured. This current is then enhanced by a gain factor proportional to the electrode capacitance. In addition, when measurements are carried out at lowfrequencies in currentmode the electrode contribution to the noise can be minimized. The performance of the electrodes and themethod is demonstrated using zebrafish hearts and glioma cell cultures.We propose that this electricalmethod is an ideal tool to measure in vitro slow and temporally synchronized events that are often involved in long range intracellular signaling. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/108920 http://hdl.handle.net/10316/108920 https://doi.org/10.1016/j.sbsr.2016.06.002 |
url |
http://hdl.handle.net/10316/108920 https://doi.org/10.1016/j.sbsr.2016.06.002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
22141804 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799134134469132288 |