Isolation and purification of complex II from proteus mirabilis strain ATCC 29245

Detalhes bibliográficos
Autor(a) principal: Shabbiri,Khadija
Data de Publicação: 2010
Outros Autores: Ahmad,Waqar, Syed,Quratulain, Adnan,Ahmad
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300032
Resumo: A respiratory complex was isolated from plasma membrane of pathogenic Proteus mirabilis strain ATCC 29245. It was identified as complex II consisting of succinate:quinone oxidoreductase (EC 1.3.5.1) containing single heme b. The complex II was purified by ion-exchange chromatography and gel filtration. The molecular weight of purified complex was 116.5 kDa and it was composed of three subunits with molecular weights of 19 kDa, 29 kDa and 68.5 kDa. The complex II contained 9.5 nmoles of cytochrome b per mg protein. Heme staining indicated that the 19 kDa subunit was cytochrome b. Its reduced form showed absorptions peaks at 557.0, 524.8 and 424.4 nm. The α-band was shifted from 557.0 nm to 556.8 nm in pyridine ferrohemochrome spectrum. The succinate: quinone oxidoreductase activity was found to be high in this microorganism.
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spelling Isolation and purification of complex II from proteus mirabilis strain ATCC 29245Proteus mirabiliscomplex IIcytochrome bA respiratory complex was isolated from plasma membrane of pathogenic Proteus mirabilis strain ATCC 29245. It was identified as complex II consisting of succinate:quinone oxidoreductase (EC 1.3.5.1) containing single heme b. The complex II was purified by ion-exchange chromatography and gel filtration. The molecular weight of purified complex was 116.5 kDa and it was composed of three subunits with molecular weights of 19 kDa, 29 kDa and 68.5 kDa. The complex II contained 9.5 nmoles of cytochrome b per mg protein. Heme staining indicated that the 19 kDa subunit was cytochrome b. Its reduced form showed absorptions peaks at 557.0, 524.8 and 424.4 nm. The α-band was shifted from 557.0 nm to 556.8 nm in pyridine ferrohemochrome spectrum. The succinate: quinone oxidoreductase activity was found to be high in this microorganism.Sociedade Brasileira de Microbiologia2010-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300032Brazilian Journal of Microbiology v.41 n.3 2010reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822010005000004info:eu-repo/semantics/openAccessShabbiri,KhadijaAhmad,WaqarSyed,QuratulainAdnan,Ahmadeng2010-06-02T00:00:00Zoai:scielo:S1517-83822010000300032Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2010-06-02T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Isolation and purification of complex II from proteus mirabilis strain ATCC 29245
title Isolation and purification of complex II from proteus mirabilis strain ATCC 29245
spellingShingle Isolation and purification of complex II from proteus mirabilis strain ATCC 29245
Shabbiri,Khadija
Proteus mirabilis
complex II
cytochrome b
title_short Isolation and purification of complex II from proteus mirabilis strain ATCC 29245
title_full Isolation and purification of complex II from proteus mirabilis strain ATCC 29245
title_fullStr Isolation and purification of complex II from proteus mirabilis strain ATCC 29245
title_full_unstemmed Isolation and purification of complex II from proteus mirabilis strain ATCC 29245
title_sort Isolation and purification of complex II from proteus mirabilis strain ATCC 29245
author Shabbiri,Khadija
author_facet Shabbiri,Khadija
Ahmad,Waqar
Syed,Quratulain
Adnan,Ahmad
author_role author
author2 Ahmad,Waqar
Syed,Quratulain
Adnan,Ahmad
author2_role author
author
author
dc.contributor.author.fl_str_mv Shabbiri,Khadija
Ahmad,Waqar
Syed,Quratulain
Adnan,Ahmad
dc.subject.por.fl_str_mv Proteus mirabilis
complex II
cytochrome b
topic Proteus mirabilis
complex II
cytochrome b
description A respiratory complex was isolated from plasma membrane of pathogenic Proteus mirabilis strain ATCC 29245. It was identified as complex II consisting of succinate:quinone oxidoreductase (EC 1.3.5.1) containing single heme b. The complex II was purified by ion-exchange chromatography and gel filtration. The molecular weight of purified complex was 116.5 kDa and it was composed of three subunits with molecular weights of 19 kDa, 29 kDa and 68.5 kDa. The complex II contained 9.5 nmoles of cytochrome b per mg protein. Heme staining indicated that the 19 kDa subunit was cytochrome b. Its reduced form showed absorptions peaks at 557.0, 524.8 and 424.4 nm. The α-band was shifted from 557.0 nm to 556.8 nm in pyridine ferrohemochrome spectrum. The succinate: quinone oxidoreductase activity was found to be high in this microorganism.
publishDate 2010
dc.date.none.fl_str_mv 2010-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300032
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300032
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822010005000004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.41 n.3 2010
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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