Fluorescence In Situ Hybridization: a potentially useful technique for detection of microorganisms on mortars
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/13231 |
Resumo: | This paper discusses the possibilities of applying Fluorescence In Situ Hybridization (FISH) to detect microorganisms on mortars, as this analytical technique has been used in different fields for the detection and identification of individual microbial cells in situ. FISH technique was applied for microbial detection on test and real mortars inoculated with fungal suspensions of S. cerevisae 396 and Nectria sp. A universal eukaryotic probe (EUK516) labelled with fluorescent dye (Cy3) was tested with different cell fixation procedures (4% (w / v) paraformaldehyde or 50% (v / v) ethanol in PBS). Positive results were obtained with FISH detection of Nectria on testing/artificial as well as authentic/historical mortars, which confirms successful application of FISH technique to a new on mortars. |
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Fluorescence In Situ Hybridization: a potentially useful technique for detection of microorganisms on mortarsFISHbiodeteriogenic agentsCultural heritageMortarsThis paper discusses the possibilities of applying Fluorescence In Situ Hybridization (FISH) to detect microorganisms on mortars, as this analytical technique has been used in different fields for the detection and identification of individual microbial cells in situ. FISH technique was applied for microbial detection on test and real mortars inoculated with fungal suspensions of S. cerevisae 396 and Nectria sp. A universal eukaryotic probe (EUK516) labelled with fluorescent dye (Cy3) was tested with different cell fixation procedures (4% (w / v) paraformaldehyde or 50% (v / v) ethanol in PBS). Positive results were obtained with FISH detection of Nectria on testing/artificial as well as authentic/historical mortars, which confirms successful application of FISH technique to a new on mortars.2015-03-11T13:06:19Z2015-03-112014-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/13231http://hdl.handle.net/10174/13231engM. González, R. Vieira, P. Nunes, T. Rosado, S. Martins, A. Candeias, A. Pereira and A. T. Caldeira, Fluorescence In Situ Hybridization: a potentially useful technique for detection of microorganisms on mortars, e-conservation Journal 2, 2014, pp. 44-522183-1335ndndndndndcandeias@uevora.ptamlp@uevora.ptatc@uevora.pt303González, MVieira, RNunes, PRosado, TMartins, SCandeias, APereira, ACaldeira, A Tinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:58:32Zoai:dspace.uevora.pt:10174/13231Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:06:40.755307Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Fluorescence In Situ Hybridization: a potentially useful technique for detection of microorganisms on mortars |
title |
Fluorescence In Situ Hybridization: a potentially useful technique for detection of microorganisms on mortars |
spellingShingle |
Fluorescence In Situ Hybridization: a potentially useful technique for detection of microorganisms on mortars González, M FISH biodeteriogenic agents Cultural heritage Mortars |
title_short |
Fluorescence In Situ Hybridization: a potentially useful technique for detection of microorganisms on mortars |
title_full |
Fluorescence In Situ Hybridization: a potentially useful technique for detection of microorganisms on mortars |
title_fullStr |
Fluorescence In Situ Hybridization: a potentially useful technique for detection of microorganisms on mortars |
title_full_unstemmed |
Fluorescence In Situ Hybridization: a potentially useful technique for detection of microorganisms on mortars |
title_sort |
Fluorescence In Situ Hybridization: a potentially useful technique for detection of microorganisms on mortars |
author |
González, M |
author_facet |
González, M Vieira, R Nunes, P Rosado, T Martins, S Candeias, A Pereira, A Caldeira, A T |
author_role |
author |
author2 |
Vieira, R Nunes, P Rosado, T Martins, S Candeias, A Pereira, A Caldeira, A T |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
González, M Vieira, R Nunes, P Rosado, T Martins, S Candeias, A Pereira, A Caldeira, A T |
dc.subject.por.fl_str_mv |
FISH biodeteriogenic agents Cultural heritage Mortars |
topic |
FISH biodeteriogenic agents Cultural heritage Mortars |
description |
This paper discusses the possibilities of applying Fluorescence In Situ Hybridization (FISH) to detect microorganisms on mortars, as this analytical technique has been used in different fields for the detection and identification of individual microbial cells in situ. FISH technique was applied for microbial detection on test and real mortars inoculated with fungal suspensions of S. cerevisae 396 and Nectria sp. A universal eukaryotic probe (EUK516) labelled with fluorescent dye (Cy3) was tested with different cell fixation procedures (4% (w / v) paraformaldehyde or 50% (v / v) ethanol in PBS). Positive results were obtained with FISH detection of Nectria on testing/artificial as well as authentic/historical mortars, which confirms successful application of FISH technique to a new on mortars. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-05-01T00:00:00Z 2015-03-11T13:06:19Z 2015-03-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/13231 http://hdl.handle.net/10174/13231 |
url |
http://hdl.handle.net/10174/13231 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
M. González, R. Vieira, P. Nunes, T. Rosado, S. Martins, A. Candeias, A. Pereira and A. T. Caldeira, Fluorescence In Situ Hybridization: a potentially useful technique for detection of microorganisms on mortars, e-conservation Journal 2, 2014, pp. 44-52 2183-1335 nd nd nd nd nd candeias@uevora.pt amlp@uevora.pt atc@uevora.pt 303 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136551799619584 |